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Materials 2019, 12(7), 1176;

Chitosan Nanoparticles Rescue Rotenone-Mediated Cell Death

Department of Chemistry & Biochemistry, The University of Texas at El Paso, El Paso, TX 79968, USA
Material Science & Engineering department, The University of Texas at El Paso, El Paso, TX 79968, USA
Author to whom correspondence should be addressed.
Received: 13 March 2019 / Revised: 1 April 2019 / Accepted: 4 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Environmentally Friendly Polymers and Polymer Composites)
PDF [7771 KB, uploaded 11 April 2019]


The aim of the present investigation was to study the anti-oxidant effect of chitosan nanoparticles on a human SH-SY5Y neuroblastoma cell line using a rotenone model to generate reactive oxygen species. Chitosan nanoparticles were synthesized using an ionotropic gelation method. The obtained nanoparticles were characterized using various analytical techniques such as Dynamic Light Scattering, Scanning Electron Microscopy, Transmission Electron Microscopy, Fourier Transmission Infrared spectroscopy and Atomic Force Microscopy. Incubation of SH-SY5Y cells with 50 µM rotenone resulted in 35–50% cell death within 24 h of incubation time. Annexin V/Propidium iodide dual staining verified that the majority of neuronal cell death occurred via the apoptotic pathway. The incubation of cells with chitosan nanoparticles reduced rotenone-initiated cytotoxicity and apoptotic cell death. Given that rotenone insult to cells causes oxidative stress, our results suggest that Chitosan nanoparticles have antioxidant and anti-apoptotic properties. Chitosan can not only serve as a novel therapeutic drug in the near future but also as a carrier for combo-therapy. View Full-Text
Keywords: chitosan (CS); anti-oxidant; anti-apoptotic activity; rotenone; Parkinson’s disease (PD) chitosan (CS); anti-oxidant; anti-apoptotic activity; rotenone; Parkinson’s disease (PD)

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Ahlawat, J.; Deemer, E.M.; Narayan, M. Chitosan Nanoparticles Rescue Rotenone-Mediated Cell Death. Materials 2019, 12, 1176.

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