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Open AccessArticle

In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells

1
Laboratory of Cellular Therapy and Hematopoietic Transplant, Internal Medicine Department, IMIB-Virgen de la Arrixaca, University of Murcia, Avenida Buenavista s/n, 30120 Murcia, Spain
2
Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
3
Departament of Special Care and Gerodontology, Faculty of Medicine, University of Murcia, 30007 Murcia, Spain
4
Endodontics Unit, Faculty of Medicine and Odontology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain
*
Author to whom correspondence should be addressed.
Materials 2019, 12(22), 3694; https://doi.org/10.3390/ma12223694 (registering DOI)
Received: 27 September 2019 / Revised: 25 October 2019 / Accepted: 6 November 2019 / Published: 8 November 2019
(This article belongs to the Special Issue Biocompatibility and Bioactivity of New Endodontic Materials)
This study aimed to analyze the biological effects of three new bioactive materials on cell survival, migration, morphology, and attachment in vitro. ACTIVA Kids BioACTIVE Restorative (Pulpdent, Watertown, MA, USA) (Activa), Ionolux (Voco, Cuxhaven, Germany), and Riva Light Cure UV (SDI, Bayswater, Australia) (Riva) were handled and conditioned with a serum-free culture medium. Stem cells from human dental pulp (hDPSCs) were exposed to material extracts, and metabolic activity, cell migration, and cell morphology were evaluated. Cell adhesion to the different materials was analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was evaluated by energy-dispersive X-ray (EDX). One-way analysis of variance followed by a Tukey test was performed (p < 0.05). Ionolux promoted a drastic reduction in metabolic activity and wound closure compared to the control (p < 0.05), whereas Activa induced adequate metabolic activity and cell migration. Moreover, SEM and immunofluorescence analysis showed abundant cells exposed to Activa. The materials showed different surface morphologies, and EDX spectra exhibited different peaks of C, O, Si, S, Ca, and F ions in glass ionomer cements. The results showed that Activa induced cell migration, cell attachment, and cell viability to a greater extent than Riva and Ionolux. View Full-Text
Keywords: dental materials; cytotoxicity; biological properties; dental pulp cells; bioactive materials dental materials; cytotoxicity; biological properties; dental pulp cells; bioactive materials
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López-García, S.; Pecci-Lloret, M.P.; Pecci-Lloret, M.R.; Oñate-Sánchez, R.E.; García-Bernal, D.; Castelo-Baz, P.; Rodríguez-Lozano, F.J.; Guerrero-Gironés, J. In Vitro Evaluation of the Biological Effects of ACTIVA Kids BioACTIVE Restorative, Ionolux, and Riva Light Cure on Human Dental Pulp Stem Cells. Materials 2019, 12, 3694.

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