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Materials 2019, 12(2), 214; https://doi.org/10.3390/ma12020214

Electrochemical Detection for Uric Acid Based on β-Lactoglobulin-Functionalized Multiwall Carbon Nanotubes Synthesis with PtNPs Nanocomposite

The Key Laboratory of Bioactive Materials Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China
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Received: 10 December 2018 / Revised: 1 January 2019 / Accepted: 7 January 2019 / Published: 10 January 2019
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Abstract

In this work, a simple and highly selective electrochemical biosensor for determination of uric acid (UA) is synthesized by using β-lactoglobulin (BLG)-functionalized multiwall carbon nanotubes (MWCNTs) and a platinum nanoparticles (PtNPs) nanocomposite. Urate oxidase (UOx) can oxidize uric acid to hydrogen peroxide and allantoin, which provides a good opportunity for electrochemical detection for UA. Under the optimized conditions, the current changes by the UOx/Bull Serum Albumin (BSA)/BLG-MWCNTs-PtNPs/Glassy Carbon (GC) electrode with the electrochemical method was proportional to the concentration of UA. According to experiments, we obtained a linear response with a concentration range from 0.02 to 0.5 mM and achieved a high sensitivity of 31.131 μA mM−1 and a low detection limit (0.8 μΜ). Meanwhile, nanoparticles improved the performance of the biosensor and combined with BLG not only prevented the accumulation of composite nanomaterials, but also provided immobilization of uricase through electrostatic adsorption. This improves the stability and gives the constructed electrode sensing interface superior performance in UA detection. View Full-Text
Keywords: β-lactoglobulin; multiwall carbon nanotubes; nanocomposite; urate oxidase; uric acid biosensor β-lactoglobulin; multiwall carbon nanotubes; nanocomposite; urate oxidase; uric acid biosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Han, B.; Pan, M.; Liu, X.; Liu, J.; Cui, T.; Chen, Q. Electrochemical Detection for Uric Acid Based on β-Lactoglobulin-Functionalized Multiwall Carbon Nanotubes Synthesis with PtNPs Nanocomposite. Materials 2019, 12, 214.

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