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In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers

1,†, 2,†, 3, 1,*,‡ and 2,4,*,‡
1
Department of Conservative Dentistry, School of Dentistry, Dental Research Institute, Pusan National University, Yangsan 50612, Korea
2
Microscope Center, Department of Conservative Dentistry and Oral Science Research Center, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea
3
BK21 PLUS Project, Yonsei University College of Dentistry, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea
4
Department of Electrical & Electronic Engineering, Yonsei University College of Engineering, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, Korea
*
Authors to whom correspondence should be addressed.
Ju Kyung Lee and Sunil Kim contributed equally to this work and share the first authorship.
Hyeon-Cheol Kim and Euiseong Kim contributed equally to this work as corresponding author.
Materials 2019, 12(15), 2411; https://doi.org/10.3390/ma12152411
Received: 4 July 2019 / Revised: 25 July 2019 / Accepted: 26 July 2019 / Published: 29 July 2019
(This article belongs to the Special Issue Contemporary Endodontic Materials)
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Abstract

The aim of this study was to assess the effect of three calcium silicate-based sealers (EndoSeal MTA, Nano-ceramic Sealer, and Wellroot ST) and two epoxy resin-based sealers (AH-Plus, AD Seal) on various aspects, such as cell viability, inflammatory response, and osteogenic potential, of human periodontal ligament stem cells (hPDLSCs). AH-Plus showed the lowest cell viability on hPDLSCs in all time periods in fresh media. In set media, hPDLSCs showed no significant differences in cell viability among all the tested materials. Wellroot ST showed the highest level of cell adhesion and the morphology of attached cells. AH-plus presented a significantly higher expression of IL-6 and IL-8 than the other sealers. AD Seal and three calcium silicate sealers showed high expression of the mesenchymal stem cell markers. ALP mRNA expression showed a significant increase in time-dependent manner on all of three calcium silicate-based sealers, which do not seem to interfere with the differentiation of hPDLSCs into osteoblasts. Based on the results from this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy resin-based sealers. Meanwhile, further and long-term clinical follow-up studies are required. View Full-Text
Keywords: biocompatibility; root canal sealer; calcium silicate; epoxy resin; cell viability; inflammatory response biocompatibility; root canal sealer; calcium silicate; epoxy resin; cell viability; inflammatory response
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Lee, J.K.; Kim, S.; Lee, S.; Kim, H.-C.; Kim, E. In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers. Materials 2019, 12, 2411.

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