Next Article in Journal
The Use of Eutectic Fe-Si-B Alloy as a Phase Change Material in Thermal Energy Storage Systems
Next Article in Special Issue
In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers
Previous Article in Journal
Design of Lightweight CFRP Automotive Part as an Alternative for Steel Part by Thickness and Lay-Up Optimization
Previous Article in Special Issue
Porosity Distribution in Apically Perforated Curved Root Canals Filled with Two Different Calcium Silicate Based Materials and Techniques: A Micro-Computed Tomography Study
Open AccessArticle

Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

1
Endodontic Department, Faculty of Dentistry, King Abdulaziz University, Jeddah 22252, Saudi Arabia
2
Endodontic Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo 12345, Egypt
3
Clinical Biochemistry Department, Faculty of Medicine-Rabigh, King Abdulaziz University, Jeddah 21589, Saudi Arabia
4
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
5
Stem Cell Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah 23839, Saudi Arabia
*
Author to whom correspondence should be addressed.
Materials 2019, 12(14), 2311; https://doi.org/10.3390/ma12142311
Received: 15 June 2019 / Revised: 15 July 2019 / Accepted: 17 July 2019 / Published: 19 July 2019
(This article belongs to the Special Issue Contemporary Endodontic Materials)
  |  
PDF [2422 KB, uploaded 19 July 2019]
  |  

Abstract

This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs. View Full-Text
Keywords: mesenchymal stem cells; root repair materials; alkaline phosphatase; mineral trioxide aggregates; endosequence; calcium silicate cement mesenchymal stem cells; root repair materials; alkaline phosphatase; mineral trioxide aggregates; endosequence; calcium silicate cement
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Edrees, H.Y.; Abu Zeid, S.T.; Atta, H.M.; AlQriqri, M.A. Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material. Materials 2019, 12, 2311.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top