Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

Edrees, Hadeel  Y.; Abu Zeid, Sawsan  T.H.; Atta, Hazem  M.; AlQriqri, Mehal  A.

doi:10.3390/ma12142311

Volume 12, Issue 14

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Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

Hadeel Y. Edrees^1,*

, Sawsan T.H. Abu Zeid^1,2, Hazem M. Atta^3,4 and Mehal A. AlQriqri⁵

Endodontic Department, Faculty of Dentistry, King Abdulaziz University, Jeddah 22252, Saudi Arabia

Endodontic Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo 12345, Egypt

Clinical Biochemistry Department, Faculty of Medicine-Rabigh, King Abdulaziz University, Jeddah 21589, Saudi Arabia

⁴

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

⁵

Stem Cell Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah 23839, Saudi Arabia

Author to whom correspondence should be addressed.

Materials 2019, 12(14), 2311; https://doi.org/10.3390/ma12142311

Received: 15 June 2019 / Revised: 15 July 2019 / Accepted: 17 July 2019 / Published: 19 July 2019

(This article belongs to the Special Issue Contemporary Endodontic Materials)

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Abstract

This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs. View Full-Text

Keywords: mesenchymal stem cells; root repair materials; alkaline phosphatase; mineral trioxide aggregates; endosequence; calcium silicate cement

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Edrees, H.Y.; Abu Zeid, S.T.; Atta, H.M.; AlQriqri, M.A. Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material. Materials 2019, 12, 2311.