Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material
1
Endodontic Department, Faculty of Dentistry, King Abdulaziz University, Jeddah 22252, Saudi Arabia
2
Endodontic Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo 12345, Egypt
3
Clinical Biochemistry Department, Faculty of Medicine-Rabigh, King Abdulaziz University, Jeddah 21589, Saudi Arabia
4
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
5
Stem Cell Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah 23839, Saudi Arabia
*
Author to whom correspondence should be addressed.
Materials 2019, 12(14), 2311; https://doi.org/10.3390/ma12142311
Received: 15 June 2019 / Revised: 15 July 2019 / Accepted: 17 July 2019 / Published: 19 July 2019
(This article belongs to the Special Issue Contemporary Endodontic Materials)
This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs.
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Keywords:
mesenchymal stem cells; root repair materials; alkaline phosphatase; mineral trioxide aggregates; endosequence; calcium silicate cement
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MDPI and ACS Style
Edrees, H.Y.; Abu Zeid, S.T.H.; Atta, H.M.; AlQriqri, M.A. Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material. Materials 2019, 12, 2311. https://doi.org/10.3390/ma12142311
AMA Style
Edrees HY, Abu Zeid STH, Atta HM, AlQriqri MA. Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material. Materials. 2019; 12(14):2311. https://doi.org/10.3390/ma12142311
Chicago/Turabian StyleEdrees, Hadeel Y.; Abu Zeid, Sawsan T.H.; Atta, Hazem M.; AlQriqri, Mehal A. 2019. "Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material" Materials 12, no. 14: 2311. https://doi.org/10.3390/ma12142311
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