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The Physicochemical Properties of Decellularized Extracellular Matrix-Coated 3D Printed Poly(ε-caprolactone) Nerve Conduits for Promoting Schwann Cells Proliferation and Differentiation

1
Graduate Institute of Basic Medical Sciences, China Medical University, Taichung 40447, Taiwan
2
Linsen Chinese Medicine and Kunming Branch, Taipei City Hospital, Taipei 10341, Taiwan
3
School of Medicine, China Medical University, Taichung 40447, Taiwan
4
3D Printing Medical Research Center, China Medical University Hospital, Taichung 40447, Taiwan
5
Master Program for Biomedical Engineering, China Medical University, Taichung 40447, Taiwan
6
Biomaterials Translational Research Center, China Medical University Hospital, Taichung 40447, Taiwan
7
Lab of Biomaterials, School of Chinese Medicine, China Medical University, Taichung 40447, Taiwan
8
Department of Bioinformatics and Medical Engineering, Asia University, Taichung 40447, Taiwan
9
School of Dentistry, China Medical University, Taichung 40447, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Materials 2018, 11(9), 1665; https://doi.org/10.3390/ma11091665
Received: 3 August 2018 / Revised: 4 September 2018 / Accepted: 6 September 2018 / Published: 9 September 2018
Although autologous nerve grafting remains the gold standard treatment for peripheral nerve injuries, alternative methods such as development of nerve guidance conduits have since emerged and evolved to counter the many disadvantages of nerve grafting. However, the efficacy and viability of current nerve conduits remain unclear in clinical trials. Here, we focused on a novel decellularized extracellular matrix (dECM) and polydopamine (PDA)-coated 3D-printed poly(ε-caprolactone) (PCL)-based conduits, whereby the PDA surface modification acts as an attachment platform for further dECM attachment. We demonstrated that dECM/PDA-coated PCL conduits possessed higher mechanical properties when compared to human or animal nerves. Such modifications were proved to affect cell behaviors. Cellular behaviors and neuronal differentiation of Schwann cells were assessed to determine for the efficacies of the conduits. There were some cell-specific neuronal markers, such as Nestin, neuron-specific class III beta-tubulin (TUJ-1), and microtubule-associated protein 2 (MAP2) analyzed by enzyme-linked immunosorbent assay, and Nestin expressions were found to be 0.65-fold up-regulated, while TUJ1 expressions were 2.3-fold up-regulated and MAP2 expressions were 2.5-fold up-regulated when compared to Ctl. The methodology of PDA coating employed in this study can be used as a simple model to immobilize dECM onto PCL conduits, and the results showed that dECM/PDA-coated PCL conduits can as a practical and clinically viable tool for promoting regenerative outcomes in larger peripheral nerve defects. View Full-Text
Keywords: autologous nerve grafting; decellularized extracellular matrix; polydopamine; poly(ε-caprolactone) conduits; neuronal differentiation autologous nerve grafting; decellularized extracellular matrix; polydopamine; poly(ε-caprolactone) conduits; neuronal differentiation
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MDPI and ACS Style

Chen, C.-C.; Yu, J.; Ng, H.-Y.; Lee, A.K.-X.; Chen, C.-C.; Chen, Y.-S.; Shie, M.-Y. The Physicochemical Properties of Decellularized Extracellular Matrix-Coated 3D Printed Poly(ε-caprolactone) Nerve Conduits for Promoting Schwann Cells Proliferation and Differentiation. Materials 2018, 11, 1665.

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