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Open AccessArticle

Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products

Department of Stomatology, University de Valencia, 46010 Valencia, Spain
School of Dentistry/Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, 30120 Murcia, Spain
School of Dentistry, Hospital Morales Meseguer 2pl. Av. Marqués de los Vélez s/n, University of Murcia, 30008 Murcia, Spain
Author to whom correspondence should be addressed.
Materials 2018, 11(7), 1098;
Received: 29 May 2018 / Revised: 13 June 2018 / Accepted: 22 June 2018 / Published: 27 June 2018
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
The purpose of this study was to evaluate the diffusion capacity and the biological effects of different bleaching products on human dental pulp stem cells (hDPSCs). The bleaching gel was applied for 90, 30 or 15 min to enamel/dentine discs that adapted in an artificial chamber. The diffusion of hydrogen peroxide (HP) was analysed by fluorometry and the diffusion products were applied to hDPSCs. Cell viability, cell migration and cell morphology assays were performed using the eluates of diffusion products. Finally, cell apoptosis and the expression of mesenchymal stem cell markers were analysed by flow cytometry. Statistical analysis was performed using analysis of variance and Kruskal–Wallis or Mann–Whitney tests (α < 0.05). Significant reductions of approximately 95% in cell viability were observed for the 3 × 15 min groups (p < 0.001), while 1 × 30 min of PerfectBleach and 1 × 90 min of PolaNight resulted in reductions of 50% and 60% in cell viability, respectively (p < 0.001). Similar results were obtained in the migration assay. Moreover, the 3 × 15 min group was associated with cell morphology alterations and reductions of >70% in cell live. Finally, hDPSCs maintained their mesenchymal phenotype in all conditions. Similar concentrations of carbamide peroxide (CP) and HP in different commercial products exhibited different biological effects on hDPSCs. View Full-Text
Keywords: bleaching products; diffusion; cytotoxicity; dental pulp; stem cells bleaching products; diffusion; cytotoxicity; dental pulp; stem cells
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Llena, C.; Collado-González, M.; Tomás-Catalá, C.J.; García-Bernal, D.; Oñate-Sánchez, R.E.; Rodríguez-Lozano, F.J.; Forner, L. Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products. Materials 2018, 11, 1098.

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