Next Article in Journal
Experimentally Achievable Accuracy Using a Digital Image Correlation Technique in measuring Small-Magnitude (<0.1%) Homogeneous Strain Fields
Next Article in Special Issue
Surface Modifications of Nanoparticles for Stability in Biological Fluids
Previous Article in Journal
High-Temperature Wettability and Interactions between Y-Containing Ni-Based Alloys and Various Oxide Ceramics
Previous Article in Special Issue
C60 Bioconjugation with Proteins: Towards a Palette of Carriers for All pH Ranges
Open AccessCommunication

Study of Fluorinated Quantum Dots-Protein Interactions at the Oil/Water Interface by Interfacial Surface Tension Changes

Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CiQUS) y Departamento de Física de Partículas, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain
Bioengineered Particles Group, CIC BiomaGUNE, 20014 San Sebastian, Spain
Fachbereich Physik and CHyN, Universität Hamburg, 22607 Hamburg, Germany
Ikerbasque, Basque Foundation for Science, 48011 Bilbao, Spain
Author to whom correspondence should be addressed.
Materials 2018, 11(5), 750;
Received: 17 April 2018 / Revised: 4 May 2018 / Accepted: 5 May 2018 / Published: 8 May 2018
(This article belongs to the Special Issue Surface Modification of Nanoparticles)
Understanding the interaction of nanoparticles with proteins and how this interaction modifies the nanoparticles’ surface is crucial before their use for biomedical applications. Since fluorinated materials are emerging as potential imaging probes and delivery vehicles, their interaction with proteins of biological interest must be studied in order to be able to predict their performance in real scenarios. It is known that fluorinated planar surfaces may repel the unspecific adsorption of proteins but little is known regarding the same process on fluorinated nanoparticles due to the scarce examples in the literature. In this context, the aim of this work is to propose a simple and fast methodology to study fluorinated nanoparticle-protein interactions based on interfacial surface tension (IFT) measurements. This technique is particularly interesting for fluorinated nanoparticles due to their increased hydrophobicity. Our study is based on the determination of IFT variations due to the interaction of quantum dots of ca. 5 nm inorganic core/shell diameter coated with fluorinated ligands (QD_F) with several proteins at the oil/water interface. Based on the results, we conclude that the presence of QD_F do not disrupt protein spontaneous film formation at the oil/water interface. Even if at very low concentrations of proteins the film formation in the presence of QD_F shows a slower rate, the final interfacial tension reached is similar to that obtained in the absence of QD_F. The differential behaviour of the studied proteins (bovine serum albumin, fibrinogen and apotransferrin) has been discussed on the basis of the adsorption affinity of each protein towards DCM/water interface and their different sizes. Additionally, it has been clearly demonstrated that the proposed methodology can serve as a complementary technique to other reported direct and indirect methods for the evaluation of nanoparticle-protein interactions at low protein concentrations. View Full-Text
Keywords: interfacial tension; quantum dots; fluorine; protein-nanoparticle interaction; protein corona interfacial tension; quantum dots; fluorine; protein-nanoparticle interaction; protein corona
Show Figures

Figure 1

MDPI and ACS Style

Carrillo-Carrión, C.; Gallego, M.; Parak, W.J.; Carril, M. Study of Fluorinated Quantum Dots-Protein Interactions at the Oil/Water Interface by Interfacial Surface Tension Changes. Materials 2018, 11, 750.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop