Next Article in Journal
Effect of Plasma Treatment and Its Post Process Duration on Shear Bonding Strength and Antibacterial Effect of Dental Zirconia
Next Article in Special Issue
Effect of Tartaric Acid on the Printable, Rheological and Mechanical Properties of 3D Printing Sulphoaluminate Cement Paste
Previous Article in Journal
Spray Deposition of Ag Nanowire–Graphene Oxide Hybrid Electrodes for Flexible Polymer–Dispersed Liquid Crystal Displays
Open AccessArticle

Dual-Emission Fluorescent Microspheres for the Detection of Biothiols and Hg2+

School of Material Science and Engineering, University of Jinan, Jinan 250000, China
*
Author to whom correspondence should be addressed.
Materials 2018, 11(11), 2232; https://doi.org/10.3390/ma11112232
Received: 25 September 2018 / Revised: 7 November 2018 / Accepted: 7 November 2018 / Published: 9 November 2018
(This article belongs to the Special Issue Selected Papers from IEEE ICKII 2018)
Dual-emission nanosensor for Hg2+ detection was prepared by coupling CA-AEAPMS on the surface of RBS-doped modified silica microspheres. The CA-AEAPMS was synthesized by using N-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS) and citric acid as the main raw material. The obtained nanosensor showed characteristic fluorescence emissions of Rhodamine B (red) and CA-AEAPMS (blue) under a single excitation wavelength (360 nm). Upon binding to Hg2+, only the fluorescence of CA-AEAPMS was quenched, resulting in the ratiometric fluorescence response of the dual-emission silica microspheres. This ratiometric nanosensor exhibited good selectivity to Hg2+ over other metal ions, because of the amide groups on the surface of CA-AEAPMS serving as the Hg2+ recognition sites. The ratio of F450/F580 linearly decreased with the increasing of Hg2+ concentration in the range of 0 to 3 × 10−6 M, and a detection limit was as low as 97 nM was achieved. Then, the addition of three thiol-containing amino acids (Cys, Hcy, GSH) to the quenched fluorescence solution with Hg2+ can restore the fluorescence, and the detection limits of the three biothiols (Cys, Hcy, GSH) are 0.133 μM, 0.086 μM, and 0.123 μM, respectively. View Full-Text
Keywords: dual-emission; silica microspheres; fluorescence dual-emission; silica microspheres; fluorescence
Show Figures

Figure 1

MDPI and ACS Style

Wang, J.; Zhang, H.; Guan, R. Dual-Emission Fluorescent Microspheres for the Detection of Biothiols and Hg2+. Materials 2018, 11, 2232.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop