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Open AccessArticle

Dual-Emission Fluorescent Microspheres for the Detection of Biothiols and Hg2+

School of Material Science and Engineering, University of Jinan, Jinan 250000, China
Author to whom correspondence should be addressed.
Materials 2018, 11(11), 2232;
Received: 25 September 2018 / Revised: 7 November 2018 / Accepted: 7 November 2018 / Published: 9 November 2018
(This article belongs to the Special Issue Selected Papers from IEEE ICKII 2018)
Dual-emission nanosensor for Hg2+ detection was prepared by coupling CA-AEAPMS on the surface of RBS-doped modified silica microspheres. The CA-AEAPMS was synthesized by using N-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS) and citric acid as the main raw material. The obtained nanosensor showed characteristic fluorescence emissions of Rhodamine B (red) and CA-AEAPMS (blue) under a single excitation wavelength (360 nm). Upon binding to Hg2+, only the fluorescence of CA-AEAPMS was quenched, resulting in the ratiometric fluorescence response of the dual-emission silica microspheres. This ratiometric nanosensor exhibited good selectivity to Hg2+ over other metal ions, because of the amide groups on the surface of CA-AEAPMS serving as the Hg2+ recognition sites. The ratio of F450/F580 linearly decreased with the increasing of Hg2+ concentration in the range of 0 to 3 × 10−6 M, and a detection limit was as low as 97 nM was achieved. Then, the addition of three thiol-containing amino acids (Cys, Hcy, GSH) to the quenched fluorescence solution with Hg2+ can restore the fluorescence, and the detection limits of the three biothiols (Cys, Hcy, GSH) are 0.133 μM, 0.086 μM, and 0.123 μM, respectively. View Full-Text
Keywords: dual-emission; silica microspheres; fluorescence dual-emission; silica microspheres; fluorescence
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Wang, J.; Zhang, H.; Guan, R. Dual-Emission Fluorescent Microspheres for the Detection of Biothiols and Hg2+. Materials 2018, 11, 2232.

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