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Bone Healing in Rabbit Calvaria Defects Using a Synthetic Bone Substitute: A Histological and Micro-CT Comparative Study

Laboratory of Experimental Surgery and Surgical Research N. S. Christeas, Medical School, University of Athens, 75 M. Assias Street, Athens 115 27, Greece
Department of Periodontology and Implant Dentistry, School of Dentistry, University of Detroit Mercy, 2700 Martin Luther King Jr Boulevard, Detroit, MI 48208, USA
Manchester Molecular Pathology Innovation Centre, The University of Manchester, Nelson Street, Manchester M13 9NQ, UK
Laboratory of Research of the Musculoskeletal System, Medical School, University of Athens, 2 Nikis Street, Athens 145 61, Greece
Departments of Periodontics, Implant Dentistry, and Oral Surgery, New York University College of Dentistry, 345 E 24th Street, New York, NY 10010, USA
Author to whom correspondence should be addressed.
Materials 2018, 11(10), 2004;
Received: 31 August 2018 / Revised: 14 September 2018 / Accepted: 15 October 2018 / Published: 17 October 2018
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
PDF [2431 KB, uploaded 17 October 2018]


Bioactive alloplastic materials, like beta-tricalcium phosphate (β-TCP) and calcium sulfate (CS), have been extensively researched and are currently used in orthopedic and dental bone regenerative procedures. The purpose of this study was to compare the performance of EthOss versus a bovine xenograft and spontaneous healing. The grafting materials were implanted in standardized 8 mm circular bicortical bone defects in rabbit calvariae. A third similar defect in each animal was left empty for natural healing. Six male rabbits were used. After eight weeks of healing, the animals were euthanized and the bone tissue was analyzed using histology and micro-computed tomography (micro-CT). Defects treated with β-TCP/CS showed the greatest bone regeneration and graft resorption, although differences between groups were not statistically significant. At sites that healed spontaneously, the trabecular number was lower (p < 0.05) and trabecular separation was higher (p < 0.05), compared to sites treated with β-TCP/CS or xenograft. Trabecular thickness was higher at sites treated with the bovine xenograft (p < 0.05) compared to sites filled with β-TCP/CS or sites that healed spontaneously. In conclusion, the novel β-TCP/CS grafting material performed well as a bioactive and biomimetic alloplastic bone substitute when used in cranial defects in this animal model. View Full-Text
Keywords: bone regeneration; β-tricalcium phosphate; calcium sulfate; bone substitutes; animal study bone regeneration; β-tricalcium phosphate; calcium sulfate; bone substitutes; animal study

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Leventis, M.; Fairbairn, P.; Mangham, C.; Galanos, A.; Vasiliadis, O.; Papavasileiou, D.; Horowitz, R. Bone Healing in Rabbit Calvaria Defects Using a Synthetic Bone Substitute: A Histological and Micro-CT Comparative Study. Materials 2018, 11, 2004.

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