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Materials 2017, 10(9), 1054;

Porcine Bone Scaffolds Adsorb Growth Factors Secreted by MSCs and Improve Bone Tissue Repair

Department of Otolaryngology, Head and Neck and Maxillofacial Surgery, Sackler Faculty of Medicine, Tel-Aviv Sourasky Medical Center, Tel Aviv University, 6 Weitzman Street, 64239 Tel Aviv, Israel
Department of Biomedical Sciences, University of Padova, via G. Colombo 3, 35100 Padova, Italy
Department of Neurosciences, University of Padova, via Giustiniani 5, 35100 Padova, Italy
Department of Medical, Oral, and Biotechnological Sciences, University of Chieti-Pescara, via dei Vestini 31, 66100 Chieti, Italy
Maria Cecilia Hospital, GVM & Research, Cotignola, 48033 Ravenna, Italy
Author to whom correspondence should be addressed.
Received: 10 August 2017 / Revised: 1 September 2017 / Accepted: 5 September 2017 / Published: 8 September 2017
(This article belongs to the Special Issue Dental Implant Materials)
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An ideal tissue-engineered bone graft should have both excellent pro-osteogenesis and pro-angiogenesis properties to rapidly realize the bone regeneration in vivo. To meet this goal, in this work a porcine bone scaffold was successfully used as a Trojan horse to store growth factors produced by mesenchymal stem cells (MSCs). This new scaffold showed a time-dependent release of bioactive growth factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), in vitro. The biological effect of the growth factors-adsorbed scaffold on the in vitro commitment of MSCs into osteogenic and endothelial cell phenotypes has been evaluated. In addition, we have investigated the activity of growth factor-impregnated granules in the repair of critical-size defects in rat calvaria by means of histological, immunohistochemical, and molecular biology analyses. Based on the results of our work bone tissue formation and markers for bone and vascularization were significantly increased by the growth factor-enriched bone granules after implantation. This suggests that the controlled release of active growth factors from porcine bone granules can enhance and promote bone regeneration. View Full-Text
Keywords: bone scaffold; mesenchymal stem cells; angiogenic growth factors; delivery system; bone regeneration bone scaffold; mesenchymal stem cells; angiogenic growth factors; delivery system; bone regeneration

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Mijiritsky, E.; Ferroni, L.; Gardin, C.; Bressan, E.; Zanette, G.; Piattelli, A.; Zavan, B. Porcine Bone Scaffolds Adsorb Growth Factors Secreted by MSCs and Improve Bone Tissue Repair. Materials 2017, 10, 1054.

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