In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

Haro Durand, Luis A.; Vargas, Gabriela E.; Vera-Mesones, Rosa; Baldi, Alberto; Zago, María P.; Fanovich, María A.; Boccaccini, Aldo R.; Gorustovich, Alejandro

doi:10.3390/ma10070740

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessFeature PaperArticle

In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

^1,†,

^2,†,

²,

¹,

³,

⁴,

⁵

and

Alejandro Gorustovich

^6,*

Department of Pathology and Molecular Pharmacology, IByME-CONICET, C1428ADN Buenos Aires, Argentina

Department of Developmental Biology, National University of Salta, A4408FVY Salta, Argentina

Institute of Experimental Pathology, IPE-CONICET, A4408FVY Salta, Argentina

⁴

Research Institute for Materials Science and Technology, INTEMA-CONICET, B7608FDQ Mar del Plata, Argentina

⁵

Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany

⁶

Interdisciplinary Materials Group-IESIING-UCASAL, INTECIN UBA-CONICET, A4400EDD Salta, Argentina

Author to whom correspondence should be addressed.

^†

Both authors contributed equally to this work.

Materials 2017, 10(7), 740; https://doi.org/10.3390/ma10070740

Received: 1 June 2017 / Revised: 24 June 2017 / Accepted: 29 June 2017 / Published: 3 July 2017

(This article belongs to the Special Issue Bioactive Glasses 2017)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Since lithium (Li⁺) plays roles in angiogenesis, the localized and controlled release of Li⁺ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO₂, 24.5% Na₂O, 24.5% CaO, and 6% P₂O₅, in which Na₂O was partially substituted by 5% Li₂O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors. View Full-Text

Keywords: endothelial cell; lithium; bioactive glass

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Supplementary Material

Supplementary File 1:
Supplementary (PDF, 2302 KiB)

Share and Cite

MDPI and ACS Style

Haro Durand, L.A.; Vargas, G.E.; Vera-Mesones, R.; Baldi, A.; Zago, M.P.; Fanovich, M.A.; Boccaccini, A.R.; Gorustovich, A. In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass. Materials 2017, 10, 740. https://doi.org/10.3390/ma10070740

AMA Style

Haro Durand LA, Vargas GE, Vera-Mesones R, Baldi A, Zago MP, Fanovich MA, Boccaccini AR, Gorustovich A. In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass. Materials. 2017; 10(7):740. https://doi.org/10.3390/ma10070740

Chicago/Turabian Style

Haro Durand, Luis A., Gabriela E. Vargas, Rosa Vera-Mesones, Alberto Baldi, María P. Zago, María A. Fanovich, Aldo R. Boccaccini, and Alejandro Gorustovich. 2017. "In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass" Materials 10, no. 7: 740. https://doi.org/10.3390/ma10070740

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

Abstract

Supplementary Material

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI