Materials 2017, 10(6), 603; https://doi.org/10.3390/ma10060603
Rapid and Sensitive Detection of Bacteria Response to Antibiotics Using Nanoporous Membrane and Graphene Quantum Dot (GQDs)-Based Electrochemical Biosensors
1
Institute of Ocean Research, Zhejiang University of Technology, Hangzhou 310014, China
2
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China
3
Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong 00852, China
4
Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen 518063, China
5
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong 00852, China
6
Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China
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These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Barbara Zavan
Received: 23 April 2017 / Revised: 22 May 2017 / Accepted: 26 May 2017 / Published: 31 May 2017
(This article belongs to the Special Issue Bioapplications of Graphene Composites)
Abstract
The wide abuse of antibiotics has accelerated bacterial multiresistance, which means there is a need to develop tools for rapid detection and characterization of bacterial response to antibiotics in the management of infections. In the study, an electrochemical biosensor based on nanoporous alumina membrane and graphene quantum dots (GQDs) was developed for bacterial response to antibiotics detection. Anti-Salmonella antibody was conjugated with amino-modified GQDs by glutaraldehyde and immobilized on silanized nanoporous alumina membranes for Salmonella bacteria capture. The impedance signals across nanoporous membranes could monitor the capture of bacteria on nanoporous membranes as well as bacterial response to antibiotics. This nanoporous membrane and GQD-based electrochemical biosensor achieved rapid detection of bacterial response to antibiotics within 30 min, and the detection limit could reach the pM level. It was capable of investigating the response of bacteria exposed to antibiotics much more rapidly and conveniently than traditional tools. The capability of studying the dynamic effects of antibiotics on bacteria has potential applications in the field of monitoring disease therapy, detecting comprehensive food safety hazards and even life in hostile environment. View Full-TextKeywords:
nanoporous alumina membrane; graphene quantum dots; bacterial response to antibiotics; rapid; sensitive; electrochemical biosensor
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Ye, W.; Guo, J.; Bao, X.; Chen, T.; Weng, W.; Chen, S.; Yang, M. Rapid and Sensitive Detection of Bacteria Response to Antibiotics Using Nanoporous Membrane and Graphene Quantum Dot (GQDs)-Based Electrochemical Biosensors. Materials 2017, 10, 603.
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