Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
Abstract
:1. Introduction
2. Results
2.1. Production of Collagen–Fibrinogen Scaffolds and Films
2.1.1. Structural Analysis with Micro-CT and Scanning Electron Microscopy (SEM)
2.2. Films for Cellular Analysis
2.2.1. Cellular Adhesion and Spreading
3. Discussion
4. Materials and Methods
4.1. Study Design
4.2. Collagen–Fibrinogen Scaffolds
4.3. Cross-Linking
4.4. Analysis of Distribution of Collagen and Fibrinogen
4.5. Characterisation of Scaffold Pore Structure
4.6. Collagen-Fibrinogen Films
4.7. Cell Culture
4.7.1. Cell Adhesion Analysis
4.7.2. Cell Spreading Analysis
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Fibrinogen Content | Cross-Linking Concentration | ||||
---|---|---|---|---|---|
1% | 10% | 30% | 50% | 100% | |
0% | μCT, sIF | μCT, sIF, CA | μCT, sIF, fIF | μCT, sIF | μCT, sIF, CA, CS |
10% | μCT, sIF | μCT, sIF, CA | μCT, sIF, fIF | μCT, sIF | μCT, sIF, CA, CS |
25% | μCT, sIF | μCT, sIF, CA | μCT, sIF, fIF | μCT, sIF | μCT, sIF, CA, CS |
50% | μCT, sIF | μCT, sIF, CA | μCT, sIF, fIF | μCT, sIF | μCT, sIF, CA, CS |
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Shepherd, J.; Bax, D.; Best, S.; Cameron, R. Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration. Materials 2017, 10, 568. https://doi.org/10.3390/ma10060568
Shepherd J, Bax D, Best S, Cameron R. Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration. Materials. 2017; 10(6):568. https://doi.org/10.3390/ma10060568
Chicago/Turabian StyleShepherd, Jennifer, Daniel Bax, Serena Best, and Ruth Cameron. 2017. "Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration" Materials 10, no. 6: 568. https://doi.org/10.3390/ma10060568