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Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications

School of Mechanical and Systems Engineering, Newcastle University, Newcastle-upon-Tyne NE1 7RU, UK
Department of Mechanical and Aerospace Engineering, Politecnico di Torino, 10129 Turin, Italy
Department of Medical Engineering, School of Engineering, University of Bradford, Bradford BD7 1DP, UK
Authors to whom correspondence should be addressed.
Materials 2017, 10(7), 693;
Received: 12 April 2017 / Revised: 17 May 2017 / Accepted: 19 June 2017 / Published: 23 June 2017
(This article belongs to the Special Issue Naturally-Derived Biomaterials and Biopolymers)
Biosynthetic materials have emerged as one of the most exciting and productive fields in polymer chemistry due to their widespread adoption and potential applications in tissue engineering (TE) research. In this work, we report the synthesis of a poly(ε-caprolactone)-graft-collagen (PCL-g-Coll) copolymer. We combine its good mechanical and biodegradable PCL properties with the great biological properties of type I collagen as a functional material for TE. PCL, previously dissolved in dimethylformamide/dichloromethane mixture, and reacted with collagen using carbodiimide coupling chemistry. The synthesised material was characterised physically, chemically and biologically, using pure PCL and PCL/Coll blend samples as control. Infrared spectroscopy evidenced the presence of amide I and II peaks for the conjugated material. Similarly, XPS evidenced the presence of C–N and N–C=O bonds (8.96 ± 2.02% and 8.52 ± 0.63%; respectively) for PCL-g-Coll. Static contact angles showed a slight decrease in the conjugated sample. However, good biocompatibility and metabolic activity was obtained on PCL-g-Coll films compared to PCL and blend controls. After 3 days of culture, fibroblasts exhibited a spindle-like morphology, spreading homogeneously along the PCL-g-Coll film surface. We have engineered a functional biosynthetic polymer that can be processed by electrospinning. View Full-Text
Keywords: biosynthetic; collagen; poly(ε-caprolactone); conjugation; electrospinning; tissue engineering biosynthetic; collagen; poly(ε-caprolactone); conjugation; electrospinning; tissue engineering
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Gentile, P.; McColgan-Bannon, K.; Gianone, N.C.; Sefat, F.; Dalgarno, K.; Ferreira, A.M. Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications. Materials 2017, 10, 693.

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