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Osteoblast Cell Response on the Ti6Al4V Alloy Heat-Treated

Departamento de Ingeniería en Metalurgia y Materiales, Instituto Politécnico Nacional (ESIQIE-IPN), UPALM Zacatenco, Ciudad de México 07738, Mexico
Departamento de Ingeniería de Superficies, Corrosión y Durabilidad, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Madrid 28040, Spain
Departamento de Ingeniería Química Industrial, Instituto Politécnico Nacional (ESIQIE-IPN), UPALM Zacatenco, Ciudad de México 07738, Mexico
Author to whom correspondence should be addressed.
Academic Editor: Daolun Chen
Materials 2017, 10(4), 445;
Received: 13 March 2017 / Revised: 12 April 2017 / Accepted: 17 April 2017 / Published: 23 April 2017
(This article belongs to the Section Biomaterials)
PDF [4851 KB, uploaded 23 April 2017]


In an effort to examine the effect of the microstructural changes of the Ti6Al4V alloy, two heat treatments were carried out below (Ti6Al4V800) and above (Ti6Al4V1050) its β-phase transformation temperature. After each treatment, globular and lamellar microstructures were obtained. Saos-2 pre-osteoblast human osteosarcoma cells were seeded onto Ti6Al4V alloy disks and immersed in cell culture for 7 days. Electrochemical assays in situ were performed using OCP and EIS measurements. Impedance data show a passive behavior for the three Ti6Al4V alloys; additionally, enhanced impedance values were recorded for Ti6Al4V800 and Ti6Al4V1050 alloys. This passive behavior in culture medium is mostly due to the formation of TiO2 during their sterilization. Biocompatibility and cell adhesion were characterized using the SEM technique; Ti6Al4V as received and Ti6Al4V800 alloys exhibited polygonal and elongated morphology, whereas Ti6Al4V1050 alloy displayed a spherical morphology. Ti and O elements were identified by EDX analysis due to the TiO2 and signals of C, N and O, related to the formation of organic compounds from extracellular matrix. These results suggest that cell adhesion is more likely to occur on TiO2 formed in discrete α-phase regions (hcp) depending on its microstructure (grains). View Full-Text
Keywords: Ti6Al4V; biomaterials; microstructure; osteoblasts; heat treatment; titanium oxide Ti6Al4V; biomaterials; microstructure; osteoblasts; heat treatment; titanium oxide

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Chávez-Díaz, M.P.; Escudero-Rincón, M.L.; Arce-Estrada, E.M.; Cabrera-Sierra, R. Osteoblast Cell Response on the Ti6Al4V Alloy Heat-Treated. Materials 2017, 10, 445.

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