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Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene

1
School of Chemical Engineering, Xi’an University, Xi’an 710065, Shaanxi, China
2
Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an 710069, Shaanxi, China
3
College of Food Engineering and Nutritional Science, Shaanxi Normal University, No. 620, West Chang’an Avenue, Chang’an District, Xi’an 710119, Shaanxi, China
*
Authors to whom correspondence should be addressed.
Materials 2017, 10(10), 1139; https://doi.org/10.3390/ma10101139
Received: 29 August 2017 / Revised: 20 September 2017 / Accepted: 21 September 2017 / Published: 27 September 2017
(This article belongs to the Section Porous Materials)
Controllable preparation of nanopores to promote the performance of electrochemical biosensing interfaces has become one of the researching frontiers in biosensing. A double biocatalysis signal amplification of glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using horseradish peroxidase biosynthesized porous graphene (PGR) as the platform for the biocatalytic deposition of gold nanoparticles (AuNPs). The biosensor showed a linear range from 0.25 to 27.5 μM with a detection limit of 0.05 μM (S/N = 3) towards glucose. Furthermore, the proposed AuNPs/GOx–PGR modified glassy carbon electrode (AuNPs/GOx–PGR/GCE) achieved direct electron transfer of GOx. View Full-Text
Keywords: biological amplification; glucose oxidase; horseradish peroxidase; porous graphene; Au nanoparticles biological amplification; glucose oxidase; horseradish peroxidase; porous graphene; Au nanoparticles
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He, Y.; Zheng, J.; Wang, B.; Ren, H. Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene. Materials 2017, 10, 1139.

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