1. Introduction
Among various methods used to increase the productivity of the reservoir, chemical flooding, the gas injection method, and thermal methods are the most widely used processes for enhanced oil recovery (EOR) [
1,
2,
3,
4,
5,
6,
7]. In addition to these methods, there is an alternative technology called microbial enhanced oil recovery (MEOR). The MEOR method is a technology that uses microbial metabolism and products to improve oil production [
8,
9]. This technology is eco-friendly in that the materials used are biodegradable, and it is economical in that the cost to produce them is low. The MEOR process changes the petrophysical and/or petrochemical properties of the reservoir system depending on the microorganisms and the microbial materials [
10]. Among the microorganisms used in MEOR,
Leuconostoc mesenteroiedes is a microbial species that produces a biopolymer called dextran. It is used to block the high permeability zone, which decreases productivity due to early water breakthrough after waterflooding. Dextran improves productivity by the bypass effect for injected water by selective plugging in the reservoir.
Since the 1950s, MEOR has been applied in many countries in various ways, among which selective plugging has also been used as a major mechanism to increase oil productivity. The North Burbank Unit in Oklahoma [
11] and North Blowhorn Creek Oil Unit in Alabama [
12], USA, showed increased oil production through selective plugging. In Canada, plugging by
Leuconostoc was applied to the MEOR [
13], and a similar method was attempted for the fractured reservoir [
14]. Dutch researchers also showed the result of increasing oil productivity and improving water-oil ratio through the selective plugging effect by
Betacoccus dextranicus [
15]. In addition, MEORs using selective plugging have been applied in various fields in China, UK, Saudi Arabia, etc. [
16]. However, for the result of applying MEOR, one out of ten was evaluated as ineffective [
17] because proper application failed due to uncertainty in the method.
Various environmental factors affect the growth of microorganisms [
10,
18,
19,
20]. Temperature has the greatest influence on the growth of microbes [
21,
22,
23,
24,
25,
26,
27,
28,
29], and there are a range of possible growth temperatures and an optimum growth temperature depending on the microbial species. The effect of temperature on the rate of microbial growth is similar to the activity of an enzyme. The growth rate increases as the temperature increases to a certain point where protein denaturation occurs. The growth rate begins to decrease from the moment the temperature exceeds that temperature [
30]. Pressure affects microbial survival [
31,
32,
33,
34], and higher pressure conditions adversely affect microbial survival. The destruction of microbial cells depends on the level of pressure and the time of exposure to the pressure [
35]. Salinity is also known to affect microbial growth [
36,
37,
38,
39,
40,
41,
42,
43]. Salt concentration has a great influence on enzymatic catalysis [
44]. In general, the salinity range of the reservoir is known to be 100 mg/L to 300 g/L or more [
45]. According to Maudgalya et al. [
46], successful cases of MEOR have been reported in a reservoir where the temperature is below 200 °F and the salinity is above 1000 ppm.
A few numerical studies on selective plugging by bacteria have been conducted. Early models described microbial growth based on the Monod equation, and oil recovery was calculated using a simple fluid flow model [
47,
48,
49]. Delshad et al. [
50] depicted MEOR using UTCHEM, and Stewart and Kim [
51] simulated the selective plugging effect using a biofilm evolution-removal model. Vilcáez et al. [
52] and Surasani et al. [
53] studied
Leconostoc mesenteroides growth and plugging phenomena using a model called CrunchFlow. Recently, selective plugging simulation and oil productivity analysis have been performed using CMG STARS [
54,
55]. These previous studies have not been able to simultaneously reflect the effects of temperature, pressure, and salinity on the growth of microorganisms, and have not performed optimization considering these environmental factors.
Most of the earliest studies were limited to describing microbial metabolism in simple transport models. They were not suitable for analyzing oil productivity for complex reservoir conditions in filed scale. Only a few recent studies have attempted to analyze MEOR effects in the reservoir scale. However, studies that have evaluated and optimized considering the subsurface environment are not available yet. A typical reservoir environment is high temperature, high pressure, and high salinity, which are fatal to the growth of microorganisms. If these factors are not considered, it is impossible to accurately evaluate the MEOR performance. Therefore, this study attempted to perform an MEOR simulation considering three environmental variables at the same time.
The first objective of this study was to develop a microbial growth model that comprehensively considers the effects of temperature, pressure, and salinity through the Arrhenius equation. Experimental results were used to verify the proposed model, and comparisons with previously developed models were made. Oil recovery was compared between the models with and without reflecting the environmental impact to accurately predict the MEOR result. Optimization was performed to maximize oil recovery. This process employed a response surface methodology, and the injection scenarios were set as design parameters. In addition, the sensitivity of each parameter to oil recovery was analyzed. Finally, the MEOR results for the actual heterogeneous reservoir were shown to represent the applicability of the technology.
2. Methods
2.1. Fluid Flow in Permeable Media
In this study, the multiphase and multicomponent flow in permeable media is calculated through the conservation equations as follows [
56]:
Here, subscript is the component, subscript is the phase, subscript is the stationary phase, is the number of phases, is the number of components, is the porosity, is the saturation, is the mass fraction, is the superficial velocity, is the dispersion tensor, and is the kinetic reaction rate. The first term on the left side is the accumulation, the second term is the flux, and the right side is the source term.
Under spatially discretized conditions, CMG STARS used in this study calculates the conservation equation of flowing component
as follows:
Here, subscripts , , , are water phase, oil phase, gas phase, and layer k, respectively. The , , are mole fractions of component i in water phase, oil phase, and gas phase, respectively. is the number of neighboring regions or grid block faces, is the transmissibility, is the potential, is the component dispersibility, and is the phase rate in layer k.
2.2. Stoichiometric Equations
The model bacteria used in this study were
Leuconostoc mesenterioides. They generate a polysaccharidic biopolymer known as dextran under sucrose-rich conditions. Their metabolic mechanisms are well known, and modelling in this study was performed based on experimental results [
57,
58]. Reactions that happen during microbial metabolism were divided into four steps: (1) microbial growth, (2) sucrose hydrolysis, (3) dextran production, and (4) microbial decay.
The stoichiometric equation for microbial growth is described as follows [
52]:
Here, is sucrose, is the model bacteria, is fructose, is lactate, is acetate, is mannitol, and is ethanol.
The sucrose is hydrolyzed into glucose and fructose by microbial enzymatic reactions or is converted into dextran by the linking of glucosidic chains. The hydrolysis reaction is as follows:
and the dextran formation is as follows [
59]:
The molecular weight of dextran measured in this study was 10,053 lb/mole, and based on this, was assumed to be 6.2.
To account for the microbial decay reaction, we followed Bültemeier et al. [
60], who assumed that the dead microbes did not participate in the chemical reactions but were changed to water as follows:
2.3. Microbial Reaction Rate
In this study, the rate of microbial reactions was described using the Arrhenius equation. It is mainly used in chemical reactions instead of the Monod equation, which is widely used in environmental engineering. By adding a division factor to the general Arrhenius equation, the role of sucrose as a limiting factor is described as follows:
Here, is reaction rate, is the frequency factor, is the division factor, is the activation energy, is the gas constant, is temperature, is the number of components, is the concentration of component , is the sucrose concentration, and and are constants.
The microbial decay rate is expressed as a function related to the frequency factor and the microbial population as follows:
where
is the reaction rate for bacterial decay.
2.4. Environmental Factor Effects on Reaction Rate
2.4.1. Temperature
The cardinal temperature model proposed by Rosso et al. [
61] is expressed as follows:
Here, is the reaction rate as a function of temperature, is optimum reaction rate, is the optimum temperature for microbial growth, is the upper limit for growth temperature, and is the lower limit for growth temperature.
This temperature model is described by modifying the exponential term of the Arrhenius equation as follows [
62]:
where
is the constant of the
j-th step,
is the activation energy of the
j-th step, and
is the temperature of the
j-th step. The final form of the equation reflecting the temperature effect is given as:
2.4.2. Pressure
Basak et al. [
35] developed a decay model of
L. mesenteroides as a function of pressure. The population of microbes followed first-order kinetics during the pressure-hold time as follows:
where
is the initial number of microbes,
is the number of surviving microbes,
is the rate constant, and
is the pressure-hold time. The time taken to reduce the number of microbes to one tenth is defined as a decimal reduction time (
), and Equation (13) is as
. The relationship between pressure and decimal time is as follows:
where
is the decimal time of the
j-th step,
is the pressure of the
j-th step, and
is the negative reciprocal slope of
vs.
. Experiments showed that
and
have a linear relationship. Through this relationship,
in Equation (13) can be estimated and microbial destruction due to pressure can be calculated.
In this study, the frequency factor in Equation (7) is determined as a constant by pressure as follows:
where
is the reaction rate as affected by pressure, and
is the frequency factor for each pressure step.
is then used as a matching parameter for history match, and the value for each pressure step is derived.
2.4.3. Salinity
Leroi et al. [
63] presented the reaction rate as a function of NaCl concentration based on the cardinal model as follows:
where
is the reaction rate as a function of NaCl concentration,
is NaCl concentration,
is the optimum NaCl concentration for microbial growth, and
is the upper limit of NaCl concentration for microbial growth. Since the optimal NaCl concentration of the model bacteria was close to zero [
64,
65,
66], Equation (16) can be defined as follows:
In this study, the growth inhibition by NaCl is described as the backward reaction of Equation (2), and the rate is expressed as follows:
where
is the backward reaction rate by NaCl, and
is a constant.
2.5. Permeability Reduction Model
The generated dextran is insoluble, and it is adsorbed or trapped in the pores, which lowers the permeability of the reservoir. In this study, dextran was assumed to be a solid phase, and the change in pore volume caused by the produced dextran affected the change in permeability as follows:
where
is the changed porosity,
is the initial porosity,
is the concentration of the solid phase in pore space,
is the density of the solid phase,
is the changed permeability,
is the initial permeability, and
is the multiplier factor.
represents the volume of the solid phase in the pore space. When the volume occupied by the solid phase in the pores increases, the porosity decreases, which leads to a decrease in permeability.
2.6. History Matching Error
The history matching error means the relative difference between the measured data and simulation results for each objective function. The matching error can be estimated as follows [
62]:
Here,
is the history matching error,
and
are time and elapsed time, respectively,
is the simulated result,
is the measured result, and
is the normalization scale. In this study, the normalization scale is the maximum of the following quantities:
where
is the measured maximum change, and
is the measurement error. The value of
means that if the simulated result is between (measured value −
) and (measured value +
), the result of this matching is satisfactory. That is,
is half the absolute error range.
2.7. Proxy Model for Sensitivity Analysis and Optimization
In this study, an optimization process was performed using the response surface methodology (RSM) to maximize the oil recovery. The RSM is a method to search the correlation between input parameter and response (objective function). The main idea is to use a proxy model to represent the original simulation result. Linear or quadratic form is mainly used to make a proxy model, and the latter is applied in this study. This proxy model is also used to analyze the sensitivity of each parameter to the objective function. A quadratic polynomial method was applied to build a proxy model. The quadratic model is expressed as follows [
62,
67]:
Here, is the objective function (response), expresses the linear effect of parameters, are the quadratic effects of the parameters, indicate the interaction effects of parameters, is the intercept, are the coefficients of linear terms, are the coefficients of quadratic terms, and are the coefficients of interaction terms.
The sensitivity indicates how much a parameter change affects the objective function change. The greater the value of coefficient (effect estimate), the greater the sensitivity of the parameter to the objective function. At this time, each parameter must have scale invariance. All parameters have an average value of zero and are set to vary from −1 to 1.