Trace Element Supplementation and Enzyme Addition to Enhance Biogas Production by Anaerobic Digestion of Chicken Litter
Abstract
:1. Introduction
2. Materials and Methods
2.1. Substrate, Inoculum and Reagents
2.2. Biomethane Potential (BMP) Assay
2.3. Analytical Methods
2.4. Gas Analysis
2.5. One-Way ANOVA and Pearson Correlation
3. Results
3.1. Substrate and Inoculum Characteristics
3.2. Biogas and Methane Yield
3.2.1. Enzyme, Trace Element and Selenium Batch Assays
3.2.2. Enzyme, Trace Elements and Selenium Combination Batch Assays
3.2.3. Substrate Pretreatment with Enzyme Batch Assays
3.3. Specific Methane Yield (SMY)
3.4. Comparison of Parameters
4. Conclusions
- Enzyme treatment without the addition of TE and Se gave the highest biogas and methane yield.
- The results from the experiment highlighted that TE and Se alone did not improve the SMY significantly, while the enzyme treatment did. The results indicated that the concentration of TE and Se were inhibitory for the added enzyme but not for the microbial enzymes, as individual TE and Se assays exhibited a higher SMY than the control.
- Moreover, the SMYs were lower for combination treatment as compared to enzyme treatment alone. This was opposite to the hypothesis that synergistic effects of enzyme with TE and Se would result in a higher yield than enzyme treatment alone.
- Pretreatment of CL with an enzyme prior to the BMP assay also resulted in a lower yield than enzyme treatment. However, enzyme pretreatment still performed better than trace element supplementation.
- Despite the increased methane yields recorded for enzyme-pre-treated assays in comparison with the controls and non-treated assays, these gains were concurrent with increased TAN levels at assay conclusion, indicating that this additional step may not lead to desirable operating conditions in continuous processes.
Author Contributions
Funding
Conflicts of Interest
References
- Molaey, R.; Bayrakdar, A.; Çalli, B. Long-Term Influence of Trace Element Deficiency on Anaerobic Mono-Digestion of Chicken Manure. J. Environ. Manag. 2018, 223, 743–748. [Google Scholar] [CrossRef] [PubMed]
- Bowen, B.; Lynch, D.; Lynch, D.; Henihan, A.M.; Leahy, J.J.; McDonnell, K. Biosecurity on Poultry Farms from On-Farm Fluidized Bed Combustion and Energy Recovery from Poultry Litter. Sustainability 2010, 2, 2135–2143. [Google Scholar] [CrossRef] [Green Version]
- Thyagarajan, D.; Barathi, M.; Sakthivadivu, R. Scope of Poultry Waste Utilization. IOSR J. Agric. Vet. Sci. 2013, 6, 29–35. [Google Scholar] [CrossRef]
- Shen, J.; Jun, Z. Optimization of Methane Production in Anaerobic Co-Digestion of Poultry Litter and Wheat Straw at Different Percentages of Total Solid and Volatile Solid Using a Developed Response Surface Model. J. Environ. Sci. Health Part A 2016, 51, 325–334. [Google Scholar] [CrossRef] [PubMed]
- Kelleher, B.P.; Leahy, J.J.; Henihan, A.M.; O’dwyer, T.F.; Sutton, D.; Leahy, M.J. Advances in Poultry Litter Disposal Technology—A Review. Bioresour. Technol. 2002, 83, 27–36. [Google Scholar] [CrossRef]
- Keskin, T.; Kubra, A.; Duygu, K.; Nuri, A. The Determination of the Trace Element Effects on Basal Medium by Using the Statistical Optimization Approach for Biogas Production from Chicken Manure. Waste Biomass Valorization 2018, 1–10. [Google Scholar] [CrossRef]
- Markou, G. Improved Anaerobic Digestion Performance and Biogas Production from Poultry Litter after Lowering Its Nitrogen Content. Bioresour. Technol. 2015, 196, 726–730. [Google Scholar] [CrossRef]
- Mao, C.; Yongzhong, F.; Xiaojiao, W.; Guangxin, R. Review on Research Achievements of Biogas from Anaerobic Digestion. Renew. Sustain. Energy Rev. 2015, 45, 540–555. [Google Scholar] [CrossRef]
- Ariunbaatar, J.; Esposito, G.; Yeh, D.H.; Lens, P.N. Enhanced Anaerobic Digestion of Food Waste by Supplementing Trace Elements: Role of Selenium (VI) and Iron (II). Front. Environ. Sci. 2016, 4. [Google Scholar] [CrossRef] [Green Version]
- Cai, Y.; Zehui, Z.; Yubin, Z.; Yue, Z.; Shiyu, G.; Zongjun, C.; Xiaofen, W. Effects of Molybdenum, Selenium and Manganese Supplementation on the Performance of Anaerobic Digestion and the Characteristics of Bacterial Community in Acidogenic Stage. Bioresour. Technol. 2018, 266, 166–175. [Google Scholar] [CrossRef]
- FitzGerald, J.A.; Wall, D.M.; Jackson, S.A.; Murphy, J.D.; Dobson, A.D. Trace Element Supplementation Is Associated with Increases in Fermenting Bacteria in Biogas Mono-Digestion of Grass Silage. Renew. Energy 2019, 138, 980–986. [Google Scholar] [CrossRef]
- Molaey, R.; Bayrakdar, A.; Sürmeli, R.Ö.; Çalli, B. Influence of trace element supplementation on anaerobic digestion of chicken manure: Linking process stability to methanogenic population dynamics. J. Clean. Prod. 2018, 181, 794–800. [Google Scholar] [CrossRef]
- Demirel, B.; Scherer, P. Trace Element Requirements of Agricultural Biogas Digesters during Biological Conversion of Renewable Biomass to Methane. Biomass Bioenergy 2011, 35, 992–998. [Google Scholar] [CrossRef]
- Molaey, R.; Alper, B.; Recep, Ö.S.; Bariş, Ç. Anaerobic Digestion of Chicken Manure: Mitigating Process Inhibition at High Ammonia Concentrations by Selenium Supplementation. Biomass Bioenergy 2018, 108, 439–446. [Google Scholar] [CrossRef]
- Bhatnagar, N.; Ryan, D.; Murphy, R.; Enright, A.M. Effect of Co-Digestion Ratio and Enzyme Treatment on Biogas Production from Grass Silage and Chicken Litter. Waste Biomass Valorization 2018. [Google Scholar] [CrossRef]
- Pérez-Rodríguez, N.; García-Bernet, D.; Domínguez, J.M. Extrusion and Enzymatic Hydrolysis as Pretreatments on Corn Cob for Biogas Production. Renew. Energy 2017, 107, 597–603. [Google Scholar] [CrossRef]
- Müller, L.; Jörg, K.; Jürgen, P.; Jan, L.; Michael, N.; Frank, S. Does the Addition of Proteases Affect the Biogas Yield from Organic Material in Anaerobic Digestion? Bioresour. Technol. 2016, 203, 267–271. [Google Scholar] [CrossRef]
- Ziemiński, K.; Monika, K.-W. Effect of Enzymatic Pretreatment on Anaerobic Co-Digestion of Sugar Beet Pulp Silage and Vinasse. Bioresour. Technol. 2015, 180, 274–280. [Google Scholar] [CrossRef]
- Gerhardt, M.; Vincent, P.; Markus, B. Application of Hydrolytic Enzymes in the Agricultural Biogas Production: Results from Practical Applications in Germany. Biotechnol. J. 2007, 2, 1481–1484. [Google Scholar] [CrossRef]
- Angelidaki, I.; Wendy, S. Assessment of the Anaerobic Biodegradability of Macropollutants. Rev. Environ. Sci. Biotechnol. 2004, 3, 117–129. [Google Scholar] [CrossRef]
- Coates, J.D.; Coughlan, M.F.; Colleran, E. Simple Method for the Measurement of the Hydrogenotrophic Methanogenic Activity of Anaerobic Sludges. J. Microbiol. Methods 1996, 26, 237–246. [Google Scholar] [CrossRef]
- Kaparaju, P.; Lars, E.; Irini, A. Optimisation of Biogas Production from Manure through Serial Digestion: Lab-Scale and Pilot-Scale Studies. Bioresour. Technol. 2009, 100, 701–709. [Google Scholar] [CrossRef] [PubMed]
- Wall, D.M.; Allen, E.; Straccialini, B.; O’Kiely, P.; Murphy, J.D. The Effect of Trace Element Addition to Mono-Digestion of Grass Silage at High Organic Loading Rates. Bioresour. Technol. 2014, 172, 349–355. [Google Scholar] [CrossRef] [PubMed]
- Achinas, S.; Euverink, G.J.W. Theoretical Analysis of Biogas Potential Prediction from Agricultural Waste. Resour. Effic. Technol. 2016, 2, 143–147. [Google Scholar] [CrossRef] [Green Version]
- Li, D.; Liu, S.; Mi, L.; Li, Z.; Yuan, Y.; Yan, Z.; Liu, X. Effects of Feedstock Ratio and Organic Loading Rate on the Anaerobic Mesophilic Co-Digestion of Rice Straw and Pig Manure. Bioresour. Technol. 2015, 187, 120–127. [Google Scholar] [CrossRef]
- Li, Y.; Zhang, R.; Liu, X.; Chen, C.; Xiao, X.; Feng, L.; He y Liu, G. Evaluating Methane Production from Anaerobic Mono- and Co-Digestion of Kitchen Waste, Corn Stover, and Chicken Manure. Energy Fuels 2013, 27, 2085–2091. [Google Scholar] [CrossRef]
- Marchioro, V.; Steinmetz, R.L.; do Amaral, A.C.; Gaspareto, T.C.; Treichel, H.; Kunz, A. Poultry Litter Solid State Anaerobic Digestion: Effect of Digestate Recirculation Intervals and Substrate/Inoculum Ratios on Process Efficiency. Front. Sustain. Food Syst. 2018, 2. [Google Scholar] [CrossRef]
- Zahan, Z.; Georgiou, S.; Muster, T.H.; Othman, M.Z. Semi-Continuous Anaerobic Co-Digestion of Chicken Litter with Agricultural and Food Wastes: A Case Study on the Effect of Carbon/Nitrogen Ratio, Substrates Mixing Ratio and Organic Loading. Bioresour. Technol. 2018, 270, 245–254. [Google Scholar] [CrossRef]
- Matheri, A.N.; Ndiweni, S.N.; Belaid, M.; Muzenda, E.; Hubert, R. Optimising Biogas Production from Anaerobic Co-Digestion of Chicken Manure and Organic Fraction of Municipal Solid Waste. Renew. Sustain. Energy Rev. 2017, 80, 756–764. [Google Scholar] [CrossRef]
- Speda, J.; Johansson, M.A.; Odnell, A.; Karlsson, M. Enhanced Biomethane Production Rate and Yield from Lignocellulosic Ensiled Forage Ley by in Situ Anaerobic Digestion Treatment with Endogenous Cellulolytic Enzymes. Biotechnol. Biofuels 2017, 10, 129. [Google Scholar] [CrossRef]
- Rahman, M.A.; Møller, H.B.; Saha, C.K.; Alam, M.M.; Wahid, R.; Feng, L. Optimal Ratio for Anaerobic Co-Digestion of Poultry Droppings and Lignocellulosic-Rich Substrates for Enhanced Biogas Production. Energy Sustain. Dev. 2017, 39, 59–66. [Google Scholar] [CrossRef]
- Hassan, M.; Ding, W.; Shi, Z.; Zhao, S. Methane Enhancement through Co-Digestion of Chicken Manure and Thermo-Oxidative Cleaved Wheat Straw with Waste Activated Sludge: A C/N Optimization Case. Bioresour. Technol. 2016, 211, 534–541. [Google Scholar] [CrossRef] [PubMed]
- Schroyen, M.; Vervaeren, H.; Vandepitte, H.; Van Hulle, S.W.; Raes, K. Effect of Enzymatic Pretreatment of Various Lignocellulosic Substrates on Production of Phenolic Compounds and Biomethane Potential. Bioresour. Technol. 2015, 192, 696–702. [Google Scholar] [CrossRef] [PubMed]
- Adekunle, K.F.; Okolie, J.A. A Review of Biochemical Process of Anaerobic Digestion. Adv. Biosci. Biotechnol. 2015, 6, 205–212. [Google Scholar] [CrossRef] [Green Version]
- Khalid, A.; Arshad, M.; Anjum, M.; Mahmood, T.; Dawson, L. The Anaerobic Digestion of Solid Organic Waste. Waste Manag. 2011, 31, 1737–1744. [Google Scholar] [CrossRef]
- Zahan, Z.; Othman, M.Z.; Muster, T.H. Anaerobic Digestion/Co-Digestion Kinetic Potentials of Different Agro-Industrial Wastes: A Comparative Batch Study for C/N Optimisation. Waste Manag. 2018, 71, 663–674. [Google Scholar] [CrossRef]
Trace Element (TE) | TE Stock g/L | TE Concentrations in BMP Assays mg/L |
---|---|---|
FeCl2.4H2O | 2 | 10 |
H3BO3 | 0.05 | 2.5 |
ZnCl2 | 0.05 | 2.5 |
CuCl2 | 0.038 | 1.9 |
MnCl2.4H2O | 0.05 | 2.5 |
AlCl3 | 0.05 | 2.5 |
CoCl2.6H2O | 0.05 | 2.5 |
NiCl2.6H2O | 0.092 | 4.6 |
Na2SeO3.5H2O | 0.1 | 5 |
(NH4)6MoO4.4H2O | 0.05 | 2.5 |
BMP Assay Prefix | Pretreatment | Supplementation | Enzyme | TE | Se | Inoculum | CL | Total Volume |
---|---|---|---|---|---|---|---|---|
mL of Stock Solutions | mL | g | mL | |||||
E | ✗ | Enzyme | 8 | - | - | 50 | 2.5 | 58 |
TE | ✗ | Trace Elements | - | 1 | - | 50 | 2.5 | 51 |
Se | ✗ | Selenium | - | - | 1 | 50 | 2.5 | 51 |
E-TE | ✗ | Enzyme and Trace Elements | 8 | 1 | - | 50 | 2.5 | 59 |
E-Se | ✗ | Enzyme and Selenium | 8 | - | 1 | 50 | 2.5 | 59 |
PE | ✓ | Enzyme | 8 | - | - | 50 | 2.5 | 58 |
PE-Se | ✓ | Selenium | 8 | - | 1 | 50 | 2.5 | 59 |
PE-TE | ✓ | Trace Elements | 8 | 1 | - | 50 | 2.5 | 59 |
C | ✗ | Control | - | - | - | 50 | 2.5 | 50 |
+ve C | ✗ | Cellulose | - | - | - | 50 | 2.5 * | 50 |
Blank | ✗ | Inoculum, no substrates | - | - | - | 50 | - | 50 |
Physiological Characteristic | Inoculum | CL |
---|---|---|
pH | 8.3 ± 0.9 | 7.8 |
Alkalinity (mg/L) | 213.3 ± 24.9 | 133.3 ± 9.4 |
Moisture (%) | 96.3 ± 0.4 | 59.5 ± 1.6 |
mg/L | g/L | |
Total COD a | 41.6 ± 0.26 | 600 ± 50 |
TKN b | 5000 ± 277 | 207 ± 9.3 |
Total PO43− | 830 ± 10.2 | 222 ± 10.4 |
Ammonia | 1100 ± 44.5 | 1.08 ± 0.15 |
C/N c ratio | - | 10 |
Solids (%) | ||
Total solids (TS) | 3.7 ± 0.4 | 40.5 ± 1.1 |
Volatile solids (VS) | 2.5 ± 0.3 | 28.9 ± 1.9 |
Fixed solids (FS) | 30.4 ± 11.8 | 28.7 ± 2.5 |
VS/TS | 0.68 | 0.71 |
Volatile fatty acids | g/L | g/kg VS |
Acetic acid | 11.5 ± 0.28 | 700 ± 43.3 |
Butyric acid | 16.8 ± 0.42 | 1040 ± 66.4 |
Biogas Analysis | |||||||
---|---|---|---|---|---|---|---|
BMP Assay Prefix * | SBY L/kg VS | SMY L CH4/kg VS | CH4 % | ∆SBY % | ∆SMY % | BI | pH |
C | 523 ± 67.8 a | 240.7 ± 28.7 a | 46 ± 0.5 a | - | - | 45.7 | 8.1 |
Se | 634.1 ± 5.7 ad | 332.5 ± 2.7 ab | 52.9 ± 0.3 b | 21.2 | 38.1 | 63.1 | 8.1 |
TE | 575.5 ± 25.3 ac | 304.4 ± 12.9 abd | 52.9 ± 0.6 b | 10 | 26.5 | 57.8 | 8.1 |
E | 835.2 ± 48.6 b | 460.8 ± 32.3 c | 55.1 ± 0.7 b | 59.7 | 91.4 | 87.5 | 7.9 |
E-Se | 728.5 ± 63.1 bcd | 396.8 ± 34.8 bcd | 54.5 ± 0.1 b | 39.3 | 64.8 | 75.3 | 7.9 |
E-TE | 766 ±17 bd | 413.7 ± 7.9 c | 54 ± 0.2 b | 46.5 | 71.6 | 78.5 | 7.8 |
PE | 731.9 ± 22.3 bcd | 392 ± 23 bcd | 53.5 ± 2.2 b | 39.9 | 62.8 | 74.4 | 7.9 |
PE-TE | 716.3 ± 38.8 bcd | 387.7 ± 21.1 bcd | 54.1 ± 0.05 b | 36.9 | 61.1 | 73.6 | 7.8 |
PE-Se | 711.7 ± 130 bcd | 392.5 ± 74.2 bcd | 55.1 ± 0.4 b | 36.1 | 63.1 | 74.5 | 7.9 |
BMP Assay Prefix | VS % | TS % | VS/TS | VSR % | TAN g/L | VFA g/L | VFA/TAN |
---|---|---|---|---|---|---|---|
C | 2.2 | 3.5 | 0.6 | 45.3 ± 2.5 | 1.9 ± 0.28 | 23.8 ± 5.3 | 12.5 |
Se | 0.7 | 1.5 | 0.46 | 82 ± 6.2 | 2.3 ± 0.34 | 26.5 ± 2.7 | 11.5 |
TE | 1.1 | 1.5 | 0.73 | 72 ± 2.6 | 2.4 ± 0.45 | 29.6 ± 9.6 | 12.3 |
E | 0.4 | 1.8 | 0.2 | 91 ± 1.7 | 1.7 ± 0.15 | 25 ± 1.8 | 14.7 |
E-Se | 0.5 | 2.2 | 0.23 | 88 ± 1 | 1.8 ± 0.24 | 23.9 ± 0.9 | 13.2 |
E-TE | 0.6 | 2 | 0.3 | 84 ± 3 | 3.3 ± 0.23 | 23.6 ± 0.9 | 7.1 |
PE | 1.7 | 3.4 | 0.5 | 57.3 ± 2.5 | 3.2 ± 0.01 | 20.8 ± 1.4 | 6.5 |
PE-TE | 1.5 | 3.2 | 0.47 | 61.7 ± 3.2 | 3.2 ± 0.25 | 20 ± 2.8 | 6.25 |
PE-Se | 1.3 | 2.9 | 0.45 | 68 ± 6.2 | 3.5 ± 0.09 | 22.6 ± 0.7 | 6.45 |
SBY | CH4 % | SMY | TAN | TVFA | VSR | |
---|---|---|---|---|---|---|
SBY correlation p-value | 1 | 0.697 ** | 0.986 ** | 0.193 | −0.237 | 0.489 ** |
– | 0.000 | 0.000 | 0.334 | 0.235 | 0.010 | |
CH4 % correlation p-value | 0.697 ** | 1 | 0.803 ** | 0.310 | −0.040 | 0.633 ** |
0.000 | – | 0.000 | 0.116 | 0.842 | 0.000 | |
SMY correlation p-value | 0.986 ** | 0.803 ** | 1 | 0.222 | −0.212 | 0.546 ** |
0.000 | 0.000 | – | 0.266 | 0.290 | 0.003 | |
TAN correlation p-value | 0.193 | 0.310 | 0.222 | 1 | −0.419 * | −0.268 |
0.334 | 0.116 | 0.266 | – | 0.030 | 0.177 | |
TVFA correlation p-value | −0.237 | −0.040 | −0.212 | −0.419 * | 1 | 0.276 |
0.235 | 0.842 | 0.290 | 0.030 | – | 0.163 | |
VSR correlation p-value | 0.489 ** | 0.633 ** | 0.546 ** | −0.268 | 0.276 | 1 |
0.010 | 0.000 | 0.003 | 0.177 | 0.163 | – |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Bhatnagar, N.; Ryan, D.; Murphy, R.; Enright, A.-M. Trace Element Supplementation and Enzyme Addition to Enhance Biogas Production by Anaerobic Digestion of Chicken Litter. Energies 2020, 13, 3477. https://doi.org/10.3390/en13133477
Bhatnagar N, Ryan D, Murphy R, Enright A-M. Trace Element Supplementation and Enzyme Addition to Enhance Biogas Production by Anaerobic Digestion of Chicken Litter. Energies. 2020; 13(13):3477. https://doi.org/10.3390/en13133477
Chicago/Turabian StyleBhatnagar, Navodita, David Ryan, Richard Murphy, and Anne-Marie Enright. 2020. "Trace Element Supplementation and Enzyme Addition to Enhance Biogas Production by Anaerobic Digestion of Chicken Litter" Energies 13, no. 13: 3477. https://doi.org/10.3390/en13133477