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Energies 2018, 11(6), 1378; https://doi.org/10.3390/en11061378

Methodical Aspects of Biogas Production in Small-Volume Bioreactors in Laboratory Investigations

Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
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Received: 27 April 2018 / Revised: 22 May 2018 / Accepted: 25 May 2018 / Published: 29 May 2018
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Abstract

The aim of this study was to develop a methodology to investigate the biofermentation process in small-volume fermenters. Dark serum bottles with a volume of 100–120 mL, tightly sealed with a rubber septum, were used as bioreactors. The optimum measurement conditions in this type of bioreactor comprise: (i) filling two-thirds of the maximum volume with a suspension; (ii) a 2% bioreactor loading (on a dry basis) and; (iii) the daily equalization of pressure by removing the biogas through the septum pierced with a syringe needle and the intensive mixing of the remaining suspension. The methane yield (quantity and dynamics) obtained in this type of bioreactor is analogous to that of industrial bioreactors or large-scale laboratory bioreactors. The use of small-volume bioreactors that can be incubated will facilitate the preliminary selection of analysed systems and provide an indication of those that should be investigated in large-scale bioreactors. View Full-Text
Keywords: biogas; biofermentation method; energy biogas; biofermentation method; energy
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Kasprzycka, A.; Kuna, J. Methodical Aspects of Biogas Production in Small-Volume Bioreactors in Laboratory Investigations. Energies 2018, 11, 1378.

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