Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A
Abstract
:1. Introduction
2. Results
2.1. Inhibition of Endothelial PKA in Mice Results in Edema and Premature Death
2.2. Differential Response to PKA Inhibition in Different Vascular Beds
2.3. Inhibition of Endothelial PKA in Lymphatics Is Not Sufficient to Induce Edema
2.4. Changes in Blood Parameters Indicate Hepatic Defects in dnPKAiEC Mice
2.5. Gluconeogenesis but Not Albumin Expression Is Perturbed in dnPKAiEC Mice
2.6. Inhibition of Endothelial PKA Leads to Disorganization of Hepatic Vasculature
2.7. LSECs Preserve Their Differentiation State in dnPKAiEC Mice
2.8. Inhibition of Endothelial PKA Results in Cell-Autonomous Reactivation of the Angiogenic Program
3. Discussion
4. Materials and Methods
4.1. Animal Experimentation
4.2. Quantitative PCR
4.3. Immunostaining
4.4. Western Blotting
4.5. Scanning Electron Microscopy
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Target | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
Alb | GCAGATGACAGGGCGGAACTTG | CAGCAGCAATGGCAGGCAGAT |
Hnf4a | GCTGTCCTCGTAGCTTGACC | TTAAGAAGTGCTTCCGGGCT |
Hnf1b | AGGGAGGTGGTCGATGTCA | TCTGGACTGTCTGGTTGAACT |
G6pc | GTCTTGTCAGGCATTGCTGTG | CAGGTAGAATCCAAGCGCGA |
Pcx1 | GGGCGGAGCTAACATCTACC | TATACTCCAGACGCCGGACA |
Emcn | GGTTCCCAGAACAAGACTGAGA | TGGAATAGGAGGGGGTGGTT |
Lyve1 | CACTAGGCACCCAGTCCAAG | TCCAACCCATCCATAGCTGC |
Vegfr3 | CACAATCCCCATGCTCTGGT | TGTGAGGAGGCACATTCACC |
Gata4 | CCATCTCGCCTCCAGAGT | CTGGAAGACACCCCAATCTC |
Stab2 | CCAGGGATATCCAGGACGTA | TGTCCAGACGGCTACATCAA |
Esm1 | GCAAGAGGACAGTGCTGGAT | GGTGCCATAGGGACAGTCTTT |
Vegfa | AGGAGCCGAGCTCATGGA | GGGACCACTTGGCATGGTG |
Vegfc | TGTGCTTCTTGTCTCTGGCG | CCTTCAAAAGCCTTGACCTCG |
Fgf2 | TTCATAGCAAGGTACCGGTTG | AGAAGAGCGACCCACACG |
Hgf | GCGAATTGGTGTTCTGCCTG | GAGCAGACTGATCCCTAAAGCA |
Dll4 | GGCAAACTGCAGAACCACAC | TGGCTTCTCACTGTGTAACCG |
Sele | TCTATTTCCCACGATGCATTT | CTGCCAAAGCCTTCAATCAT |
Vcam1 | CTGGGAAGCTGGAACGAAGT | GCCAAACACTTGACCGTGAC |
Icam1 | TTTGAGCTGAGCGAGATCGG | AGAGGTCTCAGCTCCACACT |
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Nedvetsky, P.I.; Cornelissen, I.; Mathivet, T.; Bouleti, C.; Ou, P.; Baatsen, P.; Zhao, X.; Schuit, F.; Stanchi, F.; Mostov, K.E.; et al. Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A. Int. J. Mol. Sci. 2022, 23, 11419. https://doi.org/10.3390/ijms231911419
Nedvetsky PI, Cornelissen I, Mathivet T, Bouleti C, Ou P, Baatsen P, Zhao X, Schuit F, Stanchi F, Mostov KE, et al. Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A. International Journal of Molecular Sciences. 2022; 23(19):11419. https://doi.org/10.3390/ijms231911419
Chicago/Turabian StyleNedvetsky, Pavel I., Ivo Cornelissen, Thomas Mathivet, Claire Bouleti, Phalla Ou, Pieter Baatsen, Xiaocheng Zhao, Frans Schuit, Fabio Stanchi, Keith E. Mostov, and et al. 2022. "Vascular and Liver Homeostasis in Juvenile Mice Require Endothelial Cyclic AMP-Dependent Protein Kinase A" International Journal of Molecular Sciences 23, no. 19: 11419. https://doi.org/10.3390/ijms231911419