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Volume 20
Issue 5
10.3390/ijerph20053917
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Background:
Communication

The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil (Sitophilus oryzae)

by
Joanna Witecka
1,
Natalia Malejky-Kłusek
2,
Krzysztof Solarz
1,
Olga Pawełczyk
1,*,
Małgorzata Kłyś
2,
Aleksandra Izdebska
2,
Weronika Maślanko
3 and
Marek Asman
4,*
1
Department of Parasitology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland
2
Chair of Ecology and Environmental Protection, Institute of Biology, Pedagogical University of Cracow, Podchorążych 2 St., 30-084 Cracow, Poland
3
Department of Animal Ethology and Wildlife Management, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13 St., 20-950 Lublin, Poland
4
Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland
*
Authors to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2023, 20(5), 3917; https://doi.org/10.3390/ijerph20053917
Submission received: 13 January 2023 / Revised: 20 February 2023 / Accepted: 21 February 2023 / Published: 22 February 2023
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Abstract

Background: The rice weevil (Sitophilus oryzae) originates from subtropical and tropical areas of Asia and Africa, but it also appears on other continents, mostly as a result of trade in rice. It may occur in grain fields as well as in storage facilities, and cause allergenic reactions. The aim of this study was to identify the potential antigens in all developmental stages of S. oryzae, which may cause an allergic response in humans. Methods: Sera of 30 patients were tested for the presence of IgE antibodies to antigens from three life stages of the rice weevil. To identify protein fractions containing potential allergens, proteins collected from larvae, pupae, and adults separated by sex of S. oryzae were fractionated by SDS-PAGE. Then, they were probed with anti-human, anti-IgE monoclonal antibodies, fractionated by SDS-PAGE and detected by Western blotting. Results: In total, 26 protein fractions of males and 22 fractions of other life stages of S. oryzae (larvae, pupae, and females) positively reacted with the examined sera. Conclusions: The conducted study showed that S. oryzae may be a source of many antigens which may cause the potential allergic reactions in humans.
Keywords: Sitophilus oryzae; rice weevil; storage insects; allergy; sensitization Sitophilus oryzae; rice weevil; storage insects; allergy; sensitization

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MDPI and ACS Style

Witecka, J.; Malejky-Kłusek, N.; Solarz, K.; Pawełczyk, O.; Kłyś, M.; Izdebska, A.; Maślanko, W.; Asman, M. The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil (Sitophilus oryzae). Int. J. Environ. Res. Public Health 2023, 20, 3917. https://doi.org/10.3390/ijerph20053917

AMA Style

Witecka J, Malejky-Kłusek N, Solarz K, Pawełczyk O, Kłyś M, Izdebska A, Maślanko W, Asman M. The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil (Sitophilus oryzae). International Journal of Environmental Research and Public Health. 2023; 20(5):3917. https://doi.org/10.3390/ijerph20053917

Chicago/Turabian Style

Witecka, Joanna, Natalia Malejky-Kłusek, Krzysztof Solarz, Olga Pawełczyk, Małgorzata Kłyś, Aleksandra Izdebska, Weronika Maślanko, and Marek Asman. 2023. "The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil (Sitophilus oryzae)" International Journal of Environmental Research and Public Health 20, no. 5: 3917. https://doi.org/10.3390/ijerph20053917

APA Style

Witecka, J., Malejky-Kłusek, N., Solarz, K., Pawełczyk, O., Kłyś, M., Izdebska, A., Maślanko, W., & Asman, M. (2023). The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil (Sitophilus oryzae). International Journal of Environmental Research and Public Health, 20(5), 3917. https://doi.org/10.3390/ijerph20053917

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