DNA Barcoding of Chironomid Larvae (Diptera: Chironomidae) from Large Rivers in South Korea to Facilitate Freshwater Biomonitoring and Public Health Surveillance
Abstract
:1. Introduction
2. Materials and Methods
2.1. Larval Sampling and Morphological Identification
2.2. Molecular Analysis
3. Results
3.1. Specimen Identification
3.2. Morphological Key and Action Protocol
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Subfamily | Species | Accession Number |
---|---|---|
Tanypodinae | Procladius choreus (Meigen, 1804) | OM974448 †, OP381670, OP381671, OP381677, OP381678 |
Orthocladiinae | Hydrobaenus kondoi Saether, 1989 * | KP902814 †, OP381696 |
Propsilocerus akamusi (Tokunaga, 1938) | JN887116 †, OP381672, OP381685, OP381686, OP381690, OP381691, OP381692, OP381698, OP381700, OP381718 | |
Chironominae | Benthalia carbonaria (Meigen, 1804) | OM974371 †, JF412113 †, OP381680, OP381681, OP381682, OP381683, OP381684, OP381687, OP381688, OP381693, OP381694, OP381704, OP381706 |
Chironomus circumdatus (Kieffer, 1916) | OM974383 †, OP381679 | |
Chironomus nipponensis Tokunaga, 1940 | OM974375 †, OP381719, OP381722 | |
Chironomus plumosus (Linnaeus, 1758) | OM974379 †, OP381668, OP381669, OP381675, OP381676, OP381689, OP381705, OP381713, OP381714, OP381715, OP381716, OP381717, OP381720, OP381721, OP381723 | |
Dicrotendipes nervosus (Staeger, 1839) | OM974387 †, OP381695, OP381697 | |
Glyptotendipes tokunagai Sasa, 1979 | OM974397 †, OP381673, OP381674, OP381703, | |
Microchironomus tener (Kieffer, 1918) | OM974400 †, OP381711, OP381712 | |
Lipiniella moderata Kalugina, 1970 | OM974372 †, OP381707, OP381708, OP381709, OP381710 | |
Polypedilum nubeculosum (Meigen, 1804) | OM974421 †, OP381699 | |
Stenochironomus sp. | OP381666 | |
Tanytarsus ahyoni Ree & Jeong, 2010 | KT613731 †, OP381702 | |
Tanytarsus kiseogi Ree & Jeong, 2010 | JF412169 †, OP381701 | |
Chironominae sp. 1 | OP381663 | |
Chironominae sp. 2 | OP381664, OP381665 | |
Chironominae sp. 3 | OP381667 |
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Kang, H.J.; Baek, M.J.; Kang, J.H.; Bae, Y.J. DNA Barcoding of Chironomid Larvae (Diptera: Chironomidae) from Large Rivers in South Korea to Facilitate Freshwater Biomonitoring and Public Health Surveillance. Int. J. Environ. Res. Public Health 2022, 19, 12035. https://doi.org/10.3390/ijerph191912035
Kang HJ, Baek MJ, Kang JH, Bae YJ. DNA Barcoding of Chironomid Larvae (Diptera: Chironomidae) from Large Rivers in South Korea to Facilitate Freshwater Biomonitoring and Public Health Surveillance. International Journal of Environmental Research and Public Health. 2022; 19(19):12035. https://doi.org/10.3390/ijerph191912035
Chicago/Turabian StyleKang, Hyo Jeong, Min Jeong Baek, Ji Hyoun Kang, and Yeon Jae Bae. 2022. "DNA Barcoding of Chironomid Larvae (Diptera: Chironomidae) from Large Rivers in South Korea to Facilitate Freshwater Biomonitoring and Public Health Surveillance" International Journal of Environmental Research and Public Health 19, no. 19: 12035. https://doi.org/10.3390/ijerph191912035