Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

Liao, Wang; Cao, Xinyi; Xia, Hui; Wang, Shaokang; Sun, Guiju

doi:10.3390/ijerph191610254

Open AccessArticle

Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

by

Wang Liao

,

Xinyi Cao

,

Hui Xia

,

Shaokang Wang

and

Guiju Sun

^*

Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China

^*

Author to whom correspondence should be addressed.

Int. J. Environ. Res. Public Health 2022, 19(16), 10254; https://doi.org/10.3390/ijerph191610254

Submission received: 23 July 2022 / Revised: 15 August 2022 / Accepted: 15 August 2022 / Published: 18 August 2022

(This article belongs to the Special Issue Functional Foods and Cardiometabolic Health)

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Abstract

Pea protein is considered to be a high quality dietary protein source, but also it is an ideal raw material for the production of bioactive peptides. Although the hypoglycemic effect of pea protein hydrolysate (PPH) has been previously reported, the underlying mechanisms, in particular its effect on the hepatic gluconeogenesis, remain to be elucidated. In the present study, we found that PPH suppressed glucose production in mouse liver cell-line AML-12 cells. Although both of the gluconeogenic and insulin signaling pathways in the AML-12 cells could be regulated by PPH, the suppression of glucose production was dependent on the inhibition of the cAMP response element-binding protein (CREB)-mediated signaling in the gluconeogenic pathway, but not the activation of insulin signaling. Findings from the present study have unveiled a novel role of PPH underlying its anti-diabetic activity, which could be helpful to accelerate the development of functional foods and nutraceuticals using PPH as a starting material.

Keywords: pea protein hydrolysate; hepatic glucose production; gluconeogenic signaling; insulin signaling

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MDPI and ACS Style

Liao, W.; Cao, X.; Xia, H.; Wang, S.; Sun, G. Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells. Int. J. Environ. Res. Public Health 2022, 19, 10254. https://doi.org/10.3390/ijerph191610254

AMA Style

Liao W, Cao X, Xia H, Wang S, Sun G. Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells. International Journal of Environmental Research and Public Health. 2022; 19(16):10254. https://doi.org/10.3390/ijerph191610254

Chicago/Turabian Style

Liao, Wang, Xinyi Cao, Hui Xia, Shaokang Wang, and Guiju Sun. 2022. "Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells" International Journal of Environmental Research and Public Health 19, no. 16: 10254. https://doi.org/10.3390/ijerph191610254

APA Style

Liao, W., Cao, X., Xia, H., Wang, S., & Sun, G. (2022). Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells. International Journal of Environmental Research and Public Health, 19(16), 10254. https://doi.org/10.3390/ijerph191610254

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Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

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