Next Article in Journal
Symptom Profiles, Health-Related Quality of Life, and Clinical Blood Markers among Korean Community-Dwelling Older Adults Living with Chronic Conditions
Next Article in Special Issue
Perspectives of Nanoparticles in Male Infertility: Evidence for Induced Abnormalities in Sperm Production
Previous Article in Journal
Emotional Eating in Adults: The Role of Sociodemographics, Lifestyle Behaviors, and Self-Regulation—Findings from a U.S. National Study
Previous Article in Special Issue
Prenatal Environmental Metal Exposure and Preterm Birth: A Scoping Review
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJERPH
Volume 18
Issue 4
10.3390/ijerph18041742
ijerph-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men

by
Thea Emily Benson
1,*,
Anne Gaml-Sørensen
1,
Andreas Ernst
1,2,
Nis Brix
1,3,
Karin Sørig Hougaard
4,5,
Katia Keglberg Hærvig
4,6,
Jens Peter Ellekilde Bonde
4,6,
Sandra Søgaard Tøttenborg
6,
Christian H. Lindh
7,
Cecilia Høst Ramlau-Hansen
1 and
Gunnar Toft
8,9,*
1
Department of Public Health, Research Unit for Epidemiology, Aarhus University, 8000 Aarhus, Denmark
2
Department of Urology, Regional Hospital of West Jutland, 7500 Holstebro, Denmark
3
Department of Clinical Genetics, Aarhus University Hospital, 8200 Aarhus, Denmark
4
Department of Public Health, The Faculty of Health Sciences, University of Copenhagen, 1014 Copenhagen, Denmark
5
National Research Centre for the Working Environment, 2100 Copenhagen, Denmark
6
Department of Occupational and Environmental Medicine, Bispebjerg and Frederiksberg Hospital, 2400 Copenhagen, Denmark
7
Division of Occupational and Environmental Medicine, Lund University, 22363 Lund, Sweden
8
Department of Clinical Medicine, Department of Clinical Epidemiology, Aarhus University, 8200 Aarhus, Denmark
9
Steno Diabetes Center, Aarhus University Hospital, 8200, Aarhus, Denmark
*
Authors to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2021, 18(4), 1742; https://doi.org/10.3390/ijerph18041742
Submission received: 30 December 2020 / Revised: 4 February 2021 / Accepted: 5 February 2021 / Published: 11 February 2021
(This article belongs to the Special Issue The Environment and Reproductive Health: Feature Papers)
Versions Notes

Abstract

:
Bisphenol A (BPA) is considered an endocrine disruptor and has been associated with deleterious effects on spermatogenesis and male fertility. Bisphenol F (BPF) and S (BPS) are structurally similar to BPA, but knowledge of their effects on male fertility remains limited. In this cross–sectional study, we investigated the associations between exposure to BPA, BPF, and BPS and semen quality in 556 men 18–20 years of age from the Fetal Programming of Semen Quality (FEPOS) cohort. A urine sample was collected from each participant for determination of BPA, BPF, and BPS concentrations while a semen sample was collected to determine ejaculate volume, sperm concentration, total sperm count, sperm motility, and sperm morphology. Associations between urinary bisphenol levels (continuous and quartile–divided) and semen characteristics were estimated using a negative binomial regression model adjusting for urine creatinine concentration, alcohol intake, smoking status, body mass index (BMI), fever, sexual abstinence time, maternal pre–pregnancy BMI, and first trimester smoking, and highest parental education during first trimester. We found no associations between urinary bisphenol of semen quality in a sample of young men from the general Danish population.

1. Introduction

Infertility is a reproductive disorder defined as the inability to achieve pregnancy within twelve months of unprotected and frequent intercourse. The estimated prevalence of infertility among couples is 15%, with up to 50% attributed to male factors [1,2]. Yet, the specific etiological factors of male infertility remain elusive in many cases [3]. It has been proposed that exposure to environmental toxicants, such as endocrine disrupting chemicals, is an important contributor to male infertility [4].
Bisphenol A (BPA) is a ubiquitous endocrine disruptor mainly used in epoxy resins and polycarbonate plastics [5,6]. BPA has become a significant health concern since humans are exposed through ingestion of contaminated food and water, dermal contact, and by inhalation of polluted air [7]. In experimental in vitro and in vivo studies, BPA has exhibited both estrogenic and antiandrogenic properties with deleterious effects on spermatogenesis, thereby affecting semen quality [5,8,9,10,11,12,13]. Among male partners of couples seeking fertility treatment, several studies found that urinary BPA exposure is inversely associated with semen quality, including sperm concentration, total sperm count, and progressive sperm percentage of morphologically normal sperm [14,15,16,17,18]. The influence of BPA exposure on semen quality in the general population has only been investigated in few smaller studies with equivocal findings [19,20,21,22,23,24,25].
Although the estrogenic potency of BPA is still being discussed [26], the endocrine–disrupting properties of BPA exposure has entailed regulation of BPA production, and since 2010, BPA has been banned in several industrialised countries [27]. This led to the development of substances for substitution of BPA, e.g., the structurally similar bisphenol F (BPF) and bisphenol S (BPS). In experimental studies, BPF and BPS have been reported to display similar endocrine-disrupting properties as BPA [28,29,30], but only a single study has investigated the influence of BPS and BPF exposure, in addition to BPA, on semen characteristics in humans [31]. The study observed an inverse association between urinary BPA and BPS concentrations and ejaculate volume, sperm concentration, total sperm count and motility, but no firm conclusions could be drawn in regards to BPF [31].
We aimed to investigate the association between urinary BPA, BPF, and BPS concentrations and semen characteristics among young Danish men from the general population and hypothesised that higher urinary BPA, BPF and BPS concentrations are associated with lower semen quality characteristics.

2. Materials and Methods

2.1. Study Population and Participants

This cross-sectional study is based on the Fetal Programming of Semen Quality (FEPOS) cohort [32], a sub-study within The Danish National Birth Cohort (DNBC) [33]. Participants were young adult sons born to women enrolled in the DNBC between 1996 and 1999. An information letter was sent to the sons with residency near Copenhagen or Aarhus and with a minimum age of 18 years and 9 months. They were encouraged to participate in the FEPOS cohort if they had descended testicles, and no previous history of sterilisation or chemotherapy. A total of 21,623 men were eligible for participation, and between 2017 and December 2019, 5697 men were consecutively invited to complete an online lifestyle questionnaire, undergo a clinical examination, and provide a semen and a urine sample. Urine samples from a random subset of 556 men were analysed for bisphenols and were included for analysis. A full flowchart of the sampling strategy can be found in Supplementary Material (Figure S1: Flowchart of FEPOS Study Population). Clinical examinations were performed at the Department of Occupational and Environmental Medicine at Bispebjerg and Frederiksberg Hospital in Copenhagen, Denmark and at the Department of Occupational Medicine at Aarhus University Hospital in Aarhus, Denmark.

2.2. Urinary Bisphenol A, F and S

Participants provided a single urine sample in a bisphenol-free disposable plastic cup at one of the two hospital clinics. The urine samples were refrigerated at 3–8 °C for a maximum of 12 h and subsequently pipetted into 2 mL test tubes and stored at −80 °C. Urine samples were analysed for BPA, BPF, and BPS by liquid chromatography tandem mass spectrometry (LC-MS/MS; QTRAP 5500, AB Sciex Foster City, CA, USA) [34] at the laboratory at Occupational and Environmental Medicine, Lund University, Sweden. The laboratory in Lund is a reference laboratory for BPA analysis in urine and is part of the Erlangen Round Robin inter-laboratory control program. The laboratory was qualified as an Human Biomonitoring for Europe (HBM4EU) laboratory for the analysis of BPA, BPF, and BPS. Briefly, the urine samples were treated with ß–glucuronidase and incubated at 37 °C for 30 min prior to analysis. Labelled internal standards for each compound were used. All samples were analysed in a random order. The limits of detection (LOD) were 0.2 ng/mL for BPA, and 0.03 ng/mL for BPF and BPS. The coefficient of variance of an included quality control sample were at 8 ng/mL 6% for BPA, at 6 ng/mL 7% for BPF, and at 6 ng/mL 9% for BPS. Creatinine concentrations were determined in the urine samples with an enzymatic method at the Department of Clinical Chemistry, Skåne University Hospital, Lund, Sweden, in order to account for urine dilution.

2.3. Semen Characteristics

Participants provided a single semen sample for analysis with a recommended abstinence period of 2–4 days. The samples were collected by masturbation with ejaculation into a plastic container at home or at the clinic. If the sample was collected at home, the participants were requested to keep the semen container at 37 °C by transporting it close to the body. Upon receipt, samples were placed immediately in a heated incubation shaker (37 °C) for approximately 30 min and were processed within a maximum of 1–2 h from collection. Ejaculate volume was assessed by weight (assuming a density of 1 g per millilitre) in a pre-weighed container. Sperm motility was assessed by placing 6 µg of well-mixed semen under a cover slip on a heated (37 °C) microscope slide. In two samples, a minimum of 200 sperm cells were assessed for motility. A mean value was subsequently calculated for each motility group: progressive (PR): spermatozoa moving actively, either linearly or in a large circle, regardless of speed, non–progressive (NP): all other patterns of motility with an absence of progression, e.g., swimming in small circles, the flagellar force hardly displacing the head, or when only a flagellar beat can be observed, immotile (IM): no movement, and total motile (PR+NP) sperm cells [35]. Sperm concentration was determined by manually counting a minimum of 200 sperm cells in two aliquots of a diluted semen sample using an Improved Neubauer Hemocytometer (Paul Marienfield GmbH &Co, Lauda-Königshofen, Germany). Total sperm count was calculated by multiplying the number of spermatozoa (sperm concentration) with ejaculate volume in millilitre. Morphology was determined at the laboratory in Lund, Sweden, by estimating the percentage of normal sperm cells.
Both collection and examination of semen samples followed the World Health Organization (WHO) 2010 standards and requirements [35]. Semen samples were analysed by two trained biomedical laboratory technologists, blinded to exposure status of the participants. The technologists participated in an external quality control programme to ensure compliance with WHO guidelines and ESHIRE External Quality Assessment Scheme. All comparisons were in line with previous studies and further details can be found in Keglberg et al. (2020) [32].

2.4. Covariates and Precision Variables

Potential confounding factors were selected a priori based on their anticipated impact on the association between BPA, BPF, and BPS concentration and semen characteristics. Directed Acyclic Graphs (DAGs) were used to illustrate the hypothesised causal structure under study. In addition, precision variables were included in all models to improve precision of the analysis (Figure S2: Directed Acyclic Graph Used for Identification of Confounding Factors and Precision Variables).
Information on smoking status (non–smoker; smoker), alcohol intake (less than once a month; 1–3 times a month; 1–2 times a week; ≥3 times a week), and body mass index (BMI) (<18.5; 18.5–24.9; ≥25 kg/m2) was obtained through the self-completed FEPOS questionnaires prior to clinical examination. Information on maternal first trimester smoking status (smoker; non–smoker) and pre-pregnancy BMI (<18.5; 18.5–24.9; ≥25 kg/m2) was obtained through a telephone interview completed in the first trimester along with highest parental educational status (high grade professional; low grade professional; skilled or unskilled worker; student or economically inactive).
Precision variables included abstinence time (<2 days; 2–4 days; >4 days), spillage (yes; no), and information on incidences of fever within three months prior to sampling (yes; no) was provided by the participants in a form accompanying the semen sample container. Urine creatinine (mmol/L) was measured and included to control for urine dilution of bisphenol concentrations.

2.5. Statistical Analysis

Due to local data regulations, it is not allowed to report values based on less than five observations [36]. Thus, descriptive characteristics for the distribution of urinary bisphenol A, F, and S concentrations and semen characteristics in our study population, are presented using pseudo percentiles, which is the average of the actual percentile and the corresponding two upper and two lower values.
We compared the residuals from standard linear regressions with and without log-transformed semen characteristics to the residuals from a negative binomial regression. The negative binomial regression models were checked by comparing the observed distributions against the model-based distributions from the fitted model using quantile-quantile-plots (QQ-plots). Then, standardised deviance residuals were plotted against model-based predictions. The negative binomial regression model provided the best fit and was used to estimate crude and adjusted ratios (equivalent to percentage change) with 95% confidence intervals (95% CI) between each semen characteristic, and continuous and quartile divided (Q1–Q4) creatinine-adjusted urinary BPA, BPF, and BPS concentrations. The adjusted regression analyses included the abovementioned covariates and precision variables. In addition, the analyses of motility were also adjusted for the number of minutes from sample collection to analysis. Samples for which spillage was reported (n = 95) were excluded from the analyses of ejaculate volume and total sperm count according to standard practice [35].
Selection weights measure the inverse probability of participation and were employed to mitigate risk of selection bias due to non-selective participation [37]. Since information on several baseline characteristics was not available for non-participants in the FEPOS cohort, information on maternal characteristics from the baseline questionnaires in the DNBC was used as surrogate markers to estimate the probability of participation. We used a logistic regression analysis with participation (yes/no) as the dependant variable, and the previously mentioned maternal and parental confounders as explanatory variables for participation status. Selection weights were applied to all negative binomial regression models using robust standard errors. Data analyses were performed using STATA version 16.1 (Metrika Consulting AB, Stockholm, Sweden).

3. Results

3.1. Study Participants

A total of 556 study participants between 18 and 20 years provided semen and urine samples and were included in the analysis. Baseline characteristics are presented in Table 1. The majority had a monthly or weekly alcohol intake (82%), were non–smokers (57%), normal weight (73%), and born to non-smoking (75%) and normal weight (65%) mothers. Distributions of urinary BPA, BPF, and BPS concentrations, and semen characteristics are presented in Table 2 and Table 3.

3.2. Urinary Bisphenol Levels

BPA was detected in 95% of urine samples, while urinary BPF and BPS were detected in 92% and 72%, respectively. The median urinary creatinine concentration was 14.31 mmol/mL.

3.3. Associations Between Urinary Bisphenol Levels and Semen Characteristics

Adjusted ratios for semen characteristics according to urinary bisphenol concentrations are presented in Table 4, and did not differ notably from the crude ratios. No associations were observed between urinary BPA, BPF and BPS concentrations divided into quartiles and ejaculate volume, sperm concentration, total sperm count or sperm morphology. Modelling BPA, BPF, and BPS as continuous exposures did not reveal other findings. For BPA, the proportion of motile (PR+NP) spermatozoa was 7% (95% CI: 2%; 13%) higher in subjects with urinary concentrations in the third quartile (Q3) compared with BPA concentrations below in the lowest quartile (Q1). For BPF, the proportion of motile (PR+NP) spermatozoa was 6% (95% CI: 1%; 12%) higher for subjects with BPF concentrations in the third quartile (Q3) compared with subjects in the lowest quartile (Q1). Finally, for BPS, subjects in the third quartile (Q3) had 13% (95% CI: −23%; −1%) lower ejaculate volume (mL) compared with subjects in the lowest quartile (Q1).

4. Discussion

4.1. Key Results

This is the first study to investigate the association between urinary BPA and its analogues, BPF and BPS, and semen characteristics among a sample of young adult men from the general population. Our data did not provide evidence that exposure to BPA, BPF or BPS was associated with semen characteristics. Statistically significant estimates were only observed at intermediate levels, and although non-monotonic dose response associations have previously been observed in relation to BPA exposure [38], no previous studies have shown non-monotonic dose response associations for the specific endpoints studied, and no biological explanation seems likely to substantiate these findings. We therefore consider these results more likely to be attributable to chance findings due to multiple testing.

4.2. Strengths and Limitations

The main strength of our study is the large study sample of young men from the general population. Although the participation rate in FEPOS was 19% of which a random set of urine samples were analysed (10%), we were able to take non-participation into account by use of selection weights in the analyses. Moreover, the participants were all young men aged 18–20 years, thus their probability of having attempted to achieve pregnancy and hence be aware of their fertility is considered negligible. All subjects were most likely unaware of their level of bisphenol exposure which further minimises the risk of selection bias [39]. Urine and semen samples were analysed in blind, and routine internal and external quality control was performed revealing high precision and repeatability, hence, diminishing random measurement error.
Due to the cross-sectional design, we relied on a single urine and semen sample from each participant. As the spermatogenic cycle lasts for approximately 90 days, the measured urine levels of bisphenols could have been determined outside sensitive time points [19]. Some studies have found large between-day variability in urinary BPA levels [40,41], but Mahalingaiah et al. (2008) report that a single urine sample may adequately predict long-term exposure [42]. We might also expect a certain degree of intra-individual variation in semen characteristics that cannot be accounted for in the present study. This variation could occur due to random biological variation or the fact that spermatogenesis is more vulnerable to exogenous factors during specific phases, both arguing for repeated sampling as a more reliable indicator for semen quality characteristics. Nevertheless, two studies investigated intra-individual variations of semen characteristics and provided evidence that a single sample may in fact be representative [43,44].
Finally, a major and unique strength in our cross-sectional study was that we were able to control for factors during prenatal life. For instance, maternal smoking and overweight have been suggested to impair gonad development in male offspring affecting semen quality in the long term [45,46,47], and were therefore controlled for in the statistical analyses.

4.3. Findings in Relation to Other Studies

Both in vitro and human studies have reported altered semen characteristics in regard to BPA exposure [48,49]. However, findings from human epidemiological studies remain equivocal which could partly be explained by the disparity in exposure levels (Table S1: Overview of Epidemiological Studies Investigating the Associations Between Bisphenols and Semen Characteristics). Furthermore, we still lack evidence on whether BPF and BPS have the same magnitude of potency as BPA.
Similar to our study, Lassen and colleagues performed a cross-sectional study in 2014 including 303 young Danish men attending military service, and examined the association between urinary BPA and semen quality [23]. Although their median BPA concentration was 2.5-fold higher than ours, they only found a statistically inverse association between urinary BPA and percentage of progressive motile sperm cells, while findings for other semen characteristics were not significant [23]. In a Spanish cross-sectional study by Adoamnei et al. including 215 male students aged 18–23, a negative association between urinary BPA and sperm concentration (mill./mL) (ß = −0.04), and total sperm count (mill.) (ß = −0.05) was reported, while other semen parameters were unaffected [19]. Furthermore, no statistically significant associations between urinary BPA concentrations and semen characteristics were reported in an American cross-sectional study by Mendiola et al. (2010) which included 375 male partners of pregnant women with a median urinary BPA concentration of 1.5 ng/mL [24]. This is in line with findings in a more recent cross-sectional study among 105 men recruited from an Italian fertility clinic with remarkably greater median urinary BPA concentration of 0.1 µg/gCr [7].
A wider range of semen quality measures were affected in the cross-sectional study by Meeker et al. (2010) who reported an inverse association between urinary BPA concentrations and sperm concentration, abnormal sperm morphology, and motile sperm among 190 American male partners of couples seeking fertility treatment [15]. Since the design and median urinary BPA concentration is equal to ours (1.30 ng/mL), we suspect that the equivocal findings among the current literature could be explained by other factors than urinary BPA-levels, as discussed below.
A prospective follow-up design may better capture BPA effects in relevant exposure window, since BPA has a short half-life compared to the duration of the spermatogenic cycle of about three months. Goldstone et al. (2015) performed a prospective cohort study including 501 male partners of couples attending fertility treatment. A single urine sample (median BPA = 1.62 ng/mL) and two semen samples were collected at the participants homes. No associations were reported despite separation in time for semen sampling [22]. A similar sampling strategy was used by Pollard et al. (2019) who collected multiple urine samples (GM = 2.5 ng/mL) from 161 fertile men using a home-based kit. No statistically significant associations were observed [25]. Contrarily, Li et al. (2011) performed a prospective cohort study on 218 factory workers from four regions in China by collecting two urine and two semen samples (pre– and post–shift). They found an inverse association between urinary BPA and sperm concentrations, total sperm count, and motility. Differences were only statistically significant when the study population was restricted to those who were highly exposed to BPA through their occupation and had a median urinary BPA concentration of 38.7 µg/gCr compared to factory workers with lower BPA exposure (median urinary BPA of 1.4 µg/gCr) [21].
The effects of BPA exposure may also be explained by the degree of infertility among men being studied. This has been illustrated by the Czechish research group Vitku et al. (2015; 2016) who investigated BPA concentrations in plasma and seminal fluid in men with different degrees of infertility. They found that seminal BPA levels increased with increasing severity of infertility, and that plasma levels of BPA were significantly higher in the groups of slightly and moderately infertile men in comparison with healthy men, and also severely infertile men [17,18]. According to the authors, one possible reason for not finding a clear dose–response relationship between BPA plasma levels and infertility is the role of other factors causing severe infertility, such as genetic or anatomic causes or the result of infections.
The findings are supported by a Greek case-control study reporting stronger association between urinary BPA levels and sperm motility in infertile compared to fertile men [50]. Mantzouki et al. (2019) reached a different conclusion when comparing serum BPA in fertile and infertile men from Greece, since no difference in semen characteristics was reported [51]. However, one must keep in mind that urinary BPA levels do, to a greater extent, reflect the excretion rate of BPA, while measurements of plasma BPA rather reflect its bioavailability. Furthermore, seminal BPA seems to be more appropriate for studying physiology and pathophysiology in the testis [17]. In addition, bisphenol levels are lower in serum than urine samples, and bisphenol F and S are often <LOD in serum, but can be determined more precisely in urine [51]. We therefore chose urine as our source for bisphenol measurements as a proxy of the internal level of exposure.
Only a single study has investigated the association between urinary BPA, and its analogues BPS and BPF, and semen characteristics in male partners of couples seeking fertility treatment. While urinary BPF concentrations were undetectable in most participants, urinary BPA (median = 0.80 ng/mL) and BPS (median = 0.30 ng/mL) concentrations were associated with lower ejaculate volume, sperm concentration, total sperm count and motility. Inverse associations between urinary BPS and semen characteristics were strongest among overweight and obese men after BMI stratification (<25 kg/m2 vs. ≥25 kg/m2) [31].
An obvious dose–response relationship between the level of bisphenol exposure and impaired semen quality cannot be fully identified in the current literature. However, a tendency towards harmful effects on semen characteristics of BPA exposure in particular seems clear, and thus the analogues, BPF and BPS, must be further investigated in larger epidemiological follow-up studies. Finally, one cannot rule out that publication bias may further contribute to the equivocal findings since fertility studies are cost-intensive and time-consuming.

5. Conclusions

In conclusion, we found no association between urinary BPA, BPF, and BPS and semen quality in young adult men from the general population. It seems likely that our exposure levels were too low to find possible effects on semen quality, and hence future studies should preferably have a clearer exposure range including a group of highly exposed subjects.

Supplementary Materials

The following are available online at https://www.mdpi.com/1660-4601/18/4/1742/s1, Figure S1: Flowchart of the FEPOS Study Population, Figure S2: Directed Acyclic Graph Used for Identification of Confounding Factors and Precision Variables, Table S1: Overview of Epidemiological Studies Investigating the Association between Bisphenols and Semen Characteristics.

Author Contributions

Conceptualization, J.P.E.B., C.H.R.-H. and G.T.; Formal analysis, T.E.B., A.E., N.B. and C.H.L.; Funding acquisition, J.P.E.B. and S.S.T.; Investigation, A.G.-S., K.K.H. and S.S.T.; Methodology, A.E., C.H.R.-H. and G.T.; Project administration, S.S.T.; Resources, J.P.E.B. and S.S.T.; Supervision, C.H.R.-H. and G.T.; Writing—original draft, T.E.B.; Writing—review and editing, T.E.B., A.G.-S., A.E., N.B., K.S.H., K.K.H., J.P.E.B., S.S.T., C.H.L., C.H.R.-H. and G.T. All authors have read and agreed to the published version of the manuscript.

Funding

This study is part of the ReproUnion collaborative study, co-financed by the European Union, Intereg V ÖKS. The FEPOS project was further funded by the Lundbeck Foundation (R170-2014-855), the Capital Region of Denmark, Doctor Sophus Carl Emil Friis og spouse Olga Doris Friis’ Fund, Axel Muusfeldt’s Foundation (2016-491), A.P. Møller Foundation (16-37) and Aarhus University. Karin Sørig Hougaard’s contribution to the present study was supported by FFIKA, Focused Research Effort on Chemicals in the Working Environment, from the Danish Government.

Institutional Review Board Statement

The establishment of the FEPOS cohort was approved by the Scientific Research Ethics Committee for Copenhagen and Frederiksberg (No. H-16015857) and the Danish Data Protection Agency (No. 2012-58-0004). Moreover, recruitment and data collection were permitted by the Steering Committee of the DNBC (Ref. no. 2016-08, 2019-19 and no. 2019-21).

Informed Consent Statement

Informed consent was obtained from all subjects included in the study.

Data Availability Statement

The FEPOS study is part of The Danish National Birth Cohort which operates an open access data policy. The DNBC Steering Committee will, however, give priority to projects already launched and agreements made. See https://www.dnbc.dk/access-to-dnbc-data for further information.

Acknowledgments

We are grateful to all participants and to biomedical laboratory technologists Marianne Lipka Flensborg and Joan Dideriksen for running the clinics and collecting data. We also thank Josefine Rahbæk Larsen for assisting with recruitment and data entry, and Cecilia Tingsmark for doing the morphology analysis. The Danish National Birth Cohort was established with a significant grant from the Danish National Research Foundation. Additional support was obtained from the Danish Regional Committees, the Pharmacy Foundation, the Egmont Foundation, the March of Dimes Birth Defects Foundation, the Health Foundation and other minor grants. The DNBC Biobank has been supported by the Novo Nordisk Foundation and the Lundbeck Foundation. The FEPOS cohort was established by a significant grant from the Interreg V Reprounion project and additional support has been obtained from the Danish Medical Research Council (SSVF 0646, 271-08-0839/06-066023, O602-01042B, 0602-02738B), the Lundbeck Foundation (195/04, R100-A9193), The Innovation Fund Denmark 0603-00294B (09-067124), the Nordea Foundation (02-2013-2014), Aarhus Ideas (AU R9-A959-13-S804), University of Copenhagen Strategic Grant (IFSV 2012), and the Danish Council for Independent Research (DFF—_4183-00594 and DFF—4183-00152).

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Boivin, J.; Bunting, L.; Collins, J.A.; Nygren, K.G. International estimates of infertility prevalence and treatment-seeking: Potential need and demand for infertility medical care. Hum. Reprod. 2007, 22, 1506–1512. [Google Scholar] [CrossRef] [Green Version]
  2. Irvine, D.S. Epidemiology and aetiology of male infertility. Hum. Reprod. 1998, 13 (Suppl. 1), 33–44. [Google Scholar] [CrossRef] [Green Version]
  3. Punab, M.; Poolamets, O.; Paju, P.; Vihljajev, V.; Pomm, K.; Ladva, R.; Korrovits, P.; Laan, M. Causes of male infertility: A 9-year prospective monocentre study on 1737 patients with reduced total sperm counts. Hum. Reprod. 2017, 32, 18–31. [Google Scholar] [CrossRef]
  4. Wong, E.W.P.; Cheng, C.Y. Impacts of environmental toxicants on male reproductive dysfunction. Trends Pharmacol. Sci. 2011, 32, 290–299. [Google Scholar] [CrossRef] [Green Version]
  5. Qiu, L.-L.; Wang, X.; Zhang, X.-H.; Zhang, Z.; Gu, J.; Liu, L.; Wang, Y.; Wang, X.; Wang, S.-L. Decreased androgen receptor expression may contribute to spermatogenesis failure in rats exposed to low concentration of bisphenol A. Toxicol. Lett. 2013, 219, 116–124. [Google Scholar] [CrossRef] [PubMed]
  6. Yin, L.; Dai, Y.; Cui, Z.; Jiang, X.; Liu, W.; Han, F.; Lin, A.; Cao, J.; Liu, J. The regulation of cellular apoptosis by the ROS-triggered PERK/EIF2alpha/chop pathway plays a vital role in bisphenol A-induced male reproductive toxicity. Toxicol. Appl. Pharmacol. 2017, 314, 98–108. [Google Scholar] [CrossRef] [PubMed]
  7. Caporossi, L.; Alteri, A.; Campo, G.; Paci, E.; Tranfo, G.; Capanna, S.; Papaleo, E.; Pigini, D.; Vigano, P.; Papaleo, B. Cross Sectional Study on Exposure to BPA and Phthalates and Semen Parameters in Men Attending a Fertility Center. Int. J. Environ. Res. Public Health 2020, 17, 489. [Google Scholar] [CrossRef] [Green Version]
  8. Jin, P.; Wang, X.; Chang, F.; Bai, Y.; Li, Y.; Zhou, R.; Chen, L. Low dose bisphenol A impairs spermatogenesis by suppressing eproductive hormone production and promoting germ cell apoptosis in adult rats. J. Biomed. Res. 2013, 27, 135–144. [Google Scholar]
  9. Chitra, K.C.; Latchoumycandane, C.; Mathur, P.P. Induction of oxidative stress by bisphenol A in the epididymal sperm of rats. Toxicology 2003, 185, 119–127. [Google Scholar] [CrossRef]
  10. Karnam, S.S.; Ghosh, R.C.; Mondal, S.; Mondal, M. Evaluation of subacute bisphenol—A toxicity on male reproductive system. Vet. World 2015, 8, 738–744. [Google Scholar] [CrossRef]
  11. Li, J.; Mao, R.; Zhou, Q.; Ding, L.; Tao, J.; Ran, M.M.; Gao, E.-S.; Yaun, W.; Wang, J.-T.; Hou, L.-F. Exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of ERK signal pathway. Toxicol. Mech. Methods 2016, 26, 180–188. [Google Scholar] [CrossRef] [PubMed]
  12. Srivastava, S.; Gupta, P. Alteration in apoptotic rate of testicular cells and sperms following administration of Bisphenol A (BPA) in Wistar albino rats. Environ. Sci. Pollut. Res. Int. 2018, 25, 21635–21643. [Google Scholar] [CrossRef]
  13. Wisniewski, P.; Romano, R.M.; Kizys, M.M.; Oliveira, K.C.; Kasamatsu, T.; Giannocco, G.; Chiamolera, M.I.; Dias-da-Silva, M.R.; Romano, M.A. Adult exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of the hypothalamic-pituitary-testicular axis. Toxicology 2015, 329, 1–9. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Knez, J.; Kranvogl, R.; Breznik, B.P.; Voncina, E.; Vlaisavljevic, V. Are urinary bisphenol A levels in men related to semen quality and embryo development after medically assisted reproduction? Fertil. Steril. 2014, 101, 215–221.e5. [Google Scholar] [CrossRef] [PubMed]
  15. Meeker, J.D.; Calafat, A.M.; Hauser, R. Urinary bisphenol A concentrations in relation to serum thyroid and reproductive hormone levels in men from an infertility clinic. Environ. Sci. Technol. 2010, 44, 1458–1463. [Google Scholar] [CrossRef] [Green Version]
  16. Radwan, M.; Wielgomas, B.; Dziewirska, E.; Radwan, P.; Kaluzny, P.; Klimowska, A.; Hanke, W.; Jurewicz, J. Urinary Bisphenol A Levels and Male Fertility. Am. J. Mens Health 2018, 12, 2144–2151. [Google Scholar] [CrossRef] [Green Version]
  17. Vitku, J.; Heracek, J.; Sosvorova, L.; Hampl, R.; Chlupacova, T.; Hill, M.; Sobotka, V.; Bicikova, M.; Starka, M. Associations of bisphenol A and polychlorinated biphenyls with spermatogenesis and steroidogenesis in two biological fluids from men attending an infertility clinic. Environ. Int. 2016, 89-90, 166–173. [Google Scholar] [CrossRef]
  18. Vitku, J.; Sosvorova, L.; Chlupacova, T.; Hampl, R.; Hill, M.; Sobotka, V. Heracek, J.; Bicikova, M.; Starka, L. Differences in bisphenol A and estrogen levels in the plasma and seminal plasma of men with different degrees of infertility. Physiol. Res. 2015, 64, S303–S311. [Google Scholar] [CrossRef] [PubMed]
  19. Adoamnei, E.; Mendiola, J.; Vela-Soria, F.; Fernandez, M.F.; Olea, N.; Jorgensen, N.; Swan, S.H.; Torres-Cantero, A.M. Urinary bisphenol A concentrations are associated with reproductive parameters in young men. Environ. Res. 2018, 161, 122–128. [Google Scholar] [CrossRef]
  20. Ji, H.; Miao, M.; Liang, H.; Shi, H.; Ruan, D.; Li, Y.; Wang, J.; Yuan, W. Exposure of environmental Bisphenol A in relation to routine sperm parameters and sperm movement characteristics among fertile men. Sci. Rep. 2018, 8, 17548. [Google Scholar] [CrossRef]
  21. Li, D.K.; Zhou, Z.; Miao, M.; He, Y.; Wang, J.; Ferber, J.; Herrinton, L.J.; Gao, E.; Yuan, W. Urine bisphenol-A (BPA) level in relation to semen quality. Fertil. Steril. 2011, 95, 625–630.e4. [Google Scholar] [CrossRef]
  22. Goldstone, A.E.; Chen, Z.; Perry, M.J.; Kannan, K.; Louis, G.M. Urinary bisphenol A and semen quality, the LIFE Study. Reprod. Toxicol. 2015, 51, 7–13. [Google Scholar] [CrossRef] [PubMed]
  23. Lassen, T.H.; Frederiksen, H.; Jensen, T.K.; Petersen, J.H.; Joensen, U.N.; Main, K.M.; Skakkebaek, N.E.; Juul, A.; Jørgensen, N.; Andersson, A.-M. Urinary bisphenol A levels in young men: Association with reproductive hormones and semen quality. Environ. Health Perspect. 2014, 122, 478–484. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Mendiola, J.; Jorgensen, N.; Andersson, A.M.; Calafat, A.M.; Ye, X.; Redmon, J.B.; Drobins, E.Z.; Wang, C.; Sparks, A.; Thurston, S.W.; et al. Are environmental levels of bisphenol a associated with reproductive function in fertile men? Environ. Health Perspect. 2010, 118, 1286–1291. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Pollard, S.H.; Cox, K.J.; Blackburn, B.E.; Wilkins, D.G.; Carrell, D.T.; Stanford, J.B.; Porucznik, C.A. Male exposure to bisphenol A (BPA) and semen quality in the Home Observation of Periconceptional Exposures (HOPE) cohort. Reprod. Toxicol. 2019, 90, 82–87. [Google Scholar] [CrossRef]
  26. Sharpe, R.M. Is it time to end concerns over the estrogenic effects of bisphenol A? Toxicol. Sci. 2010, 114, 1–4. [Google Scholar] [CrossRef] [Green Version]
  27. Zamkowska, D.; Karwacka, A.; Jurewicz, J.; Radwan, M. Environmental exposure to non-persistent endocrine disrupting chemicals and semen quality: An overview of the current epidemiological evidence. Int. J. Occup. Med. Environ. Health 2018, 31, 377–414. [Google Scholar] [CrossRef]
  28. Ullah, A.; Pirzada, M.; Afsar, T.; Razak, S.; Almajwal, A.; Jahan, S. Effect of bisphenol F, an analog of bisphenol A, on the reproductive functions of male rats. Environ. Health Prev. Med. 2019, 24, 41. [Google Scholar] [CrossRef]
  29. Ullah, A.; Pirzada, M.; Jahan, S.; Ullah, H.; Shaheen, G.; Rehman, H.; Siddiqui, M.F.; Butt, M.A. Bisphenol A and its analogs bisphenol B, bisphenol F, and bisphenol S: Comparative in vitro and in vivo studies on the sperms and testicular tissues of rats. Chemosphere 2018, 209, 508–516. [Google Scholar] [CrossRef] [PubMed]
  30. Ullah, A.; Pirzada, M.; Jahan, S.; Ullah, H.; Turi, N.; Ullah, W.; Siddiqui, M.F.; Zakria, M.; Lodhi, K.Z.; Khan, M.M. Impact of low-dose chronic exposure to bisphenol A and its analogue bisphenol B, bisphenol F and bisphenol S on hypothalamo-pituitary-testicular activities in adult rats: A focus on the possible hormonal mode of action. Food Chem. Toxicol. 2018, 121, 24–36. [Google Scholar] [CrossRef] [PubMed]
  31. Ghayda, R.A.; Williams, P.L.; Chavarro, J.E.; Ford, J.B.; Souter, I.; Calafat, A.M.; Hauser, R.; Minguez-Alarcón, L. Urinary bisphenol S concentrations: Potential predictors of and associations with semen quality parameters among men attending a fertility center. Environ. Int. 2019, 131, 105050. [Google Scholar] [CrossRef]
  32. Hærvig, K.K.; Bonde, J.P.; Ramlau-Hansen, C.H.; Toft, G.; Hougaard, K.S.; Specht, I.O.; Giwercman, A.; Nybo Andersen, A.M.; Olsen, J.; Lindh, C.; et al. Fetal Programming of Semen Quality (FEPOS) Cohort—A DNBC Male-Offspring Cohort. Clin. Epidemiol. 2020, 12, 757–770. [Google Scholar] [CrossRef]
  33. Ernst, A.; Brix, N.; Lauridsen, L.L.B.; Strandberg-Larsen, K.; Bech, B.H.; Nohr, E.A.; Nybo Andersen, A.M.; Parner, E.T.; Merder, I.K.; Olsen, J.; et al. Cohort Profile: The Puberty Cohort in the Danish National Birth Cohort (DNBC). Int. J. Epidemiol. 2019, 49, 373–374g. [Google Scholar] [CrossRef] [PubMed]
  34. Gyllenhammar, I.; Glynn, A.; Jönsson, B.A.G.; Lindh, C.H.; Darnerud, P.O.; Svensson, K.; Lignell, S. Diverging temporal trends of human exposure to bisphenols and plastizisers, such as phthalates, caused by substitution of legacy EDCs? Environ. Res. 2017, 153, 48–54. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. The World Health Organization. WHO Labaratory Manual for the Examination and Processing of human Semen; WHO: Geneva, Switzerland, 2010. [Google Scholar]
  36. Hernan, M.A.; Hernandez-Diaz, S.; Robins, J.M. A structural approach to selection bias. Epidemiology 2004, 15, 615–625. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Vom Saal, F.S.; Vandenberg, L.N. Update on the health effects of bisphenol A: Overwhelming evidence of harm. Endocrinology 2021, 162. [Google Scholar] [CrossRef]
  38. Eustache, F.; Auger, J.; Cabrol, D.; Jouannet, P. Are volunteers delivering semen samples in fertility studies a biased population? Hum. Reprod. 2004, 19, 2831–2837. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  39. Ye, X.; Wong, L.-Y.; Bishop, A.M.; Calafat, A.M. Variability of urinary concentrations of bisphenol A in spot samples, first morning voids, and 24-hour collections. Environ. Health Perspect. 2011, 119, 983–988. [Google Scholar] [CrossRef]
  40. Lassen, T.H.; Frederiksen, H.; Jensen, T.K.; Petersen, J.H.; Main, K.M.; Skakkebæk, N.E.; Jørgensen, N.; Kløve Kranich, S.; Andersson, A.-M. Temporal variability in urinary excretion of bisphenol A and seven other phenols in spot, morning, and 24-h urine samples. Environ. Res. 2013, 126, 164–170. [Google Scholar] [CrossRef] [PubMed]
  41. Mahalingaiah, S.; Meeker, J.D.; Pearson, K.R.; Calafat, A.M.; Ye, X.; Petrozza, J.; Hauser, R. Temporal variability and predictors of urinary bisphenol A concentrations in men and women. Environ. Health Perspect. 2008, 116, 173–178. [Google Scholar] [CrossRef] [Green Version]
  42. Francavilla, F.; Barbonetti, A.; Necozione, S.; Santucci, R.; Cordeschi, G.; Macerola, B.; Francavilla, S. Within-subject variation of seminal parameters in men with infertile marriages. Int. J. Androl. 2007, 30, 174–181. [Google Scholar] [CrossRef] [PubMed]
  43. Zhu, Q.-X.; Gao, E.-S.; Pathak, N.; Wu, J.-Q.; Zhou, W.-J. Single or double semen samples: The dilemma in epidemiological studies on semen quality. Hum. Reprod. 2016, 31, 511–517. [Google Scholar] [CrossRef] [Green Version]
  44. Axelsson, J.; Rylander, L.; Rignell-Hydbom, A.; Silfver, K.Å.; Stenqvist, A.; Giwercman, A. The Impact of Paternal and Maternal Smoking on Semen Quality of Adolescent Men. PLoS ONE 2013, 8. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Ramlau-Hansen, C.H.; Nohr, E.A.; Thulstrup, A.M.; Bonde, J.P.; Storgaard, L.; Olsen, J. Is maternal obesity related to semen quality in the male offspring? A pilot study. Hum. Reprod. 2007, 22, 2758–2762. [Google Scholar] [CrossRef] [Green Version]
  46. Haervig, K.K.; Høyer, B.B.; Giwercman, A.; Hougaard, K.S.; Ramlau-Hansen, C.H.; Specht, I.O.; Tofr, G.; Bond, J.P.; Søgaard Tøttenborg, S. Fetal exposure to paternal smoking and semen quality in the adult son. Andrology 2020, 8, 1117–1125. [Google Scholar] [CrossRef] [PubMed]
  47. Chiang, C.; Mahalingam, S.; Flaws, J.A. Environmental Contaminants Affecting Fertility and Somatic Health. Semin. Reprod. Med. 2017, 35, 241–249. [Google Scholar] [CrossRef] [PubMed]
  48. Tomza-Marciniak, A.; Stepkowska, P.; Kuba, J.; Pilarczyk, B. Effect of bisphenol A on reproductive processes: A review of in vitro, in vivo and epidemiological studies. J. Appl. Toxicol. 2018, 38, 51–80. [Google Scholar] [CrossRef] [PubMed]
  49. Omran, G.A.; Gaber, H.D.; Mostafa, N.A.M.; Abdel-Gaber, R.M.; Salah, E.A. Potential hazards of bisphenol A exposure to semen quality and sperm DNA integrity among infertile men. Reprod. Toxicol. 2018, 81, 188–195. [Google Scholar] [CrossRef]
  50. Mantzouki, C.; Bliatka, D.; Iliadou, P.K.; Margeli, A.; Papassotiriou, I.; Mastorakos, G.; Kousta, E.; Goulis, D.G. Serum Bisphenol A concentrations in men with idiopathic infertility. Food Chem. Toxicol. 2019, 125, 562–565. [Google Scholar] [CrossRef] [PubMed]
  51. Liu, J.; Li, J.; Wu, Y.; Zhao, Y.; Luo, F.; Li, S.; Yang, L.; Moez, E.K.; Dinu, I.; Martin, J.W. Bisphenol A Metabolites and Bisphenol S in Paired Maternal and Cord Serum. Environ. Sci. Technol. 2017, 51, 2456–2463. [Google Scholar] [CrossRef]
Table
Table 1. Characteristics of 556 Participants from the FEPOS 1 Cohort, 2019.
Table 1. Characteristics of 556 Participants from the FEPOS 1 Cohort, 2019.
n (%)
Alcohol intake
Missing38 (7)
Less than once a month26 (5)
1–3 times a month205 (37)
1–2 times a week248 (44)
≥3 times a week39 (7)
Smoking status
Missing<5 2 (<0.9)
Non–smoker320 (58)
Smoker<235 (<42)
Body mass index (kg/m2)
<18.5 (underweight)45 (8)
18.5–24.9 (normal weight)407 (74)
>25 (overweight)102 (18)
Spillage
Missing6 (1)
No455 (82)
Yes95 (17)
Fever ≤ 90 days prior to sampling
Missing63 (11)
No402 (73)
Yes91 (16)
Abstinence time (days)
Missing<5 2 (<0.9)
<2193 (34)
2–4284 (51)
>4<78 2 (<14)
Maternal smoking status at delivery
Missing14 (2.5)
Non–smoker417 (75)
Smoker125 (22.5)
Maternal body mass index (kg/m2)
Missing25 (4.5)
<18.553 (9.5)
18.5–24.9360 (65)
>25118 (21)
Parental educational level
Missing15 (3)
High grade professional179 (32)
Low grade professional191 (34)
Skilled or unskilled worker150 (27)
Student or economically inactive21 (4)
1 Fetal Programming of Semen Quality; 2 Due to local data regulations, it is not allowed to report numbers smaller than five, which is why the numbers have been rounded.
Table
Table 2. Distribution of Urinary Bisphenol A, F and S Concentrations and Semen Characteristics among Study Participants from the FEPOS 1 Cohort, 2019.
Table 2. Distribution of Urinary Bisphenol A, F and S Concentrations and Semen Characteristics among Study Participants from the FEPOS 1 Cohort, 2019.
n5th50th95th
Bisphenol A (ng/mL)5560.221.309.90
Bisphenol F (ng/mL)556<LOD0.142.44
Bisphenol S (ng/mL)556<LOD0.061.12
Ejaculate volume (mL) *4551.02.65.4
Sperm concentration (mill./mL)5522.134.9130.5
Total sperm count (mill.) *4546.391.7390.0
Progressive and non–progressive (%)54242.371.087.0
Morphological normal sperm (%)5410.66.015.0
1 Fetal Programming of Semen Quality. All values are reported as pseudo percentiles calculated from the average of five observations. Limit of detection (LOD) is <0.2 ng/mL for bisphenol A, and <0.03 ng/mL for bisphenol F and S. * Participants of which spillage was reported were excluded from ejaculate volume and total sperm count analyses.
Table
Table 3. Pseudo Quartile (Q1–Q4) Divided Creatinine-Adjusted Urinary Bisphenol A, F, and S Concentrations among Study Participants from the FEPOS Cohort, 2019.
Table 3. Pseudo Quartile (Q1–Q4) Divided Creatinine-Adjusted Urinary Bisphenol A, F, and S Concentrations among Study Participants from the FEPOS Cohort, 2019.
BPABPFBPS
Q1 (ng/mL)<0.68<0.06<0.03
Q2 (ng/mL)0.68–1.300.06–0.140.03–0.06
Q3 (ng/mL)1.30–2.740.14–0.340.06–0.17
Q4 (ng/mL>2.74>0.34>0.017
BPA=Bispehol A; BPF=Bisphenol F and BPS=Bisphenol S.
Table
Table 4. Adjusted Ratios of Semen Characteristics According to Urinary Bisphenol A, F, and S Concentrations (ng/mL) among Study Participants (n = 556) from the FEPOS Cohort, 2019.
Table 4. Adjusted Ratios of Semen Characteristics According to Urinary Bisphenol A, F, and S Concentrations (ng/mL) among Study Participants (n = 556) from the FEPOS Cohort, 2019.
BPABPFBPS
Ratios (95% CI)Ratios (95% CI))Ratios (95% CI)
Ejaculate volume (mL)Q1ReferenceReferenceReference
Q20.97 (0.85; 1.10)0.91 (0.81; 1.03)0.97 (0.85; 1.10)
Q31.02 (0.88; 1.17)0.92 (0.80; 1.05)0.87 (0.77; 0.99)
Q41.04 (0.90; 1.19)0.96 (0.84; 1.10)1.00 (0.80; 1.13)
Continuous 1.00 (0.99; 1.01)1.00 (0.99; 1.01)1.03 (0.98; 1.08)
Sperm concentration (mill./mL)Q1ReferenceReferenceReference
Q21.10 (0.90; 1.35)1.11 (0.92; 1.35)1.06 (0.87; 1.30)
Q30.93 (0.76; 1.15)1.08 (0.87; 1.34)0.98 (0.80; 1.19)
Q41.07 (0.86; 1.34)1.05 (0.85; 1.29)1.04 (0.85; 1.28)
Continuous 1.01 (0.99; 1.02)0.99 (0.98; 1.01)0.97 (0.88; 1.07)
Total sperm count (mill.)Q1ReferenceReferenceReference
Q21.07 (0.86; 1.33)1.05 (0.84; 1.30)1.02 (0.81; 1.27)
Q30.93 (0.72; 1.19)0.97 (0.76; 1.23)0.91 (0.73; 1.14)
Q41.05 (0.82; 1.35)0.97 (0.76; 1.23)1.02 (0.81; 1.30)
Continuous 1.00 (0.99; 1.01)0.99 (0.98; 1.01)1.04 (0.95; 1.13)
Progressive and non–progressive motility (%)Q1ReferenceReferenceReference
Q21.05 (0.99; 1.11)1.04 (0.98; 1.10)1.03 (0.98; 1.09)
Q31.07 (1.02; 1.13)1.06 (1.01; 1.12)1.02 (0.97; 1.08)
Q41.04 (0.98; 1.11)1.04 (0.99; 1.11)1.02 (0.96; 1.08)
Continuous 1.00 (1.00; 1.01)1.00 (1.00; 1.01)1.00 (0.98; 1.02)
Morphological normal sperm (%)Q1ReferenceReferenceReference
Q21.03 (0.86; 1.22)1.09 (0.92; 1.30)0.98 (0.82; 1.18)
Q30.94 (0.78; 1.13)1.04 (0.86; 1.27)1.02 (0.85; 1.21)
Q41.00 (0.82; 1.21)1.13 (0.93; 1.37)1.09 (0.90; 1.31)
Continuous 1.00 (0.99; 1.02)1.00 (0.99; 1.02)1.00 (0.93; 1.07)
Estimates marked in bold indicate p < 0.05. BPA = Bispehol A; BPF = Bisphenol F and BPS = Bisphenol S.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Benson, T.E.; Gaml-Sørensen, A.; Ernst, A.; Brix, N.; Hougaard, K.S.; Hærvig, K.K.; Ellekilde Bonde, J.P.; Tøttenborg, S.S.; Lindh, C.H.; Ramlau-Hansen, C.H.; et al. Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men. Int. J. Environ. Res. Public Health 2021, 18, 1742. https://doi.org/10.3390/ijerph18041742

AMA Style

Benson TE, Gaml-Sørensen A, Ernst A, Brix N, Hougaard KS, Hærvig KK, Ellekilde Bonde JP, Tøttenborg SS, Lindh CH, Ramlau-Hansen CH, et al. Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men. International Journal of Environmental Research and Public Health. 2021; 18(4):1742. https://doi.org/10.3390/ijerph18041742

Chicago/Turabian Style

Benson, Thea Emily, Anne Gaml-Sørensen, Andreas Ernst, Nis Brix, Karin Sørig Hougaard, Katia Keglberg Hærvig, Jens Peter Ellekilde Bonde, Sandra Søgaard Tøttenborg, Christian H. Lindh, Cecilia Høst Ramlau-Hansen, and et al. 2021. "Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men" International Journal of Environmental Research and Public Health 18, no. 4: 1742. https://doi.org/10.3390/ijerph18041742

APA Style

Benson, T. E., Gaml-Sørensen, A., Ernst, A., Brix, N., Hougaard, K. S., Hærvig, K. K., Ellekilde Bonde, J. P., Tøttenborg, S. S., Lindh, C. H., Ramlau-Hansen, C. H., & Toft, G. (2021). Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men. International Journal of Environmental Research and Public Health, 18(4), 1742. https://doi.org/10.3390/ijerph18041742

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop