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Article

In Vitro Effects of Particulate Matter Associated with a Wildland Fire in the North-West of Italy

1
Department of Public Health and Pediatrics, University of Torino, 10126 Torino, Italy
2
Regional Agency for Environmental Protection of Piedmont (ARPA Piemonte), 10095 Grugliasco, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Int. J. Environ. Res. Public Health 2021, 18(20), 10812; https://doi.org/10.3390/ijerph182010812
Received: 15 September 2021 / Accepted: 9 October 2021 / Published: 14 October 2021
(This article belongs to the Special Issue Occupational/Residential Exposure to Toxic Xenobiotics)
Wildland fires, increasing in recent decades in the Mediterranean region due to climate change, can contribute to PM levels and composition. This study aimed to investigate biological effects of PM2.5 (Ø < 2.5 µm) and PM10 (Ø < 10 µm) collected near a fire occurred in the North-West of Italy in 2017 and in three other areas (urban and rural areas). Organic extracts were assessed for mutagenicity using Ames test (TA98 and TA100 strains), cell viability (WST-1 and LDH assays) and genotoxicity (Comet assay) with human bronchial cells (BEAS-2B) and estrogenic activity using a gene reporter assay (MELN cells). In all sites, high levels of PM10 and PM2.5 were measured during the fire suggesting that near and distant sites were influenced by fire pollutants. The PM10 and PM2.5 extracts induced a significant mutagenicity in all sites and the mutagenic effect was increased with respect to historical data. All extracts induced a slight increase of the estrogenic activity but a possible antagonistic activity of PM samples collected near fire was observed. No cytotoxicity or DNA damage was detected. Results confirm that fires could be relevant for human health, since they can worsen the air quality increasing PM concentrations, mutagenic and estrogenic effects. View Full-Text
Keywords: forest fire; genotoxicity; mutagenicity; endocrine disruptors; particulate matter; air pollution; cytotoxicity; biological assays; BEAS-2B cells; MELN cells forest fire; genotoxicity; mutagenicity; endocrine disruptors; particulate matter; air pollution; cytotoxicity; biological assays; BEAS-2B cells; MELN cells
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MDPI and ACS Style

Gea, M.; Bonetta, S.; Marangon, D.; Pitasi, F.A.; Armato, C.; Gilli, G.; Bert, F.; Fontana, M.; Schilirò, T. In Vitro Effects of Particulate Matter Associated with a Wildland Fire in the North-West of Italy. Int. J. Environ. Res. Public Health 2021, 18, 10812. https://doi.org/10.3390/ijerph182010812

AMA Style

Gea M, Bonetta S, Marangon D, Pitasi FA, Armato C, Gilli G, Bert F, Fontana M, Schilirò T. In Vitro Effects of Particulate Matter Associated with a Wildland Fire in the North-West of Italy. International Journal of Environmental Research and Public Health. 2021; 18(20):10812. https://doi.org/10.3390/ijerph182010812

Chicago/Turabian Style

Gea, Marta, Sara Bonetta, Daniele Marangon, Francesco A. Pitasi, Caterina Armato, Giorgio Gilli, Fabrizio Bert, Marco Fontana, and Tiziana Schilirò. 2021. "In Vitro Effects of Particulate Matter Associated with a Wildland Fire in the North-West of Italy" International Journal of Environmental Research and Public Health 18, no. 20: 10812. https://doi.org/10.3390/ijerph182010812

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