Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats

Mokhtar, Mohd Helmy; Giribabu, Nelli; Salleh, Naguib

doi:10.3390/ijerph17072293

Open AccessArticle

Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats

by

Mohd Helmy Mokhtar

¹

,

Nelli Giribabu

² and

Naguib Salleh

^2,*

¹

Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia

²

Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia

^*

Author to whom correspondence should be addressed.

Int. J. Environ. Res. Public Health 2020, 17(7), 2293; https://doi.org/10.3390/ijerph17072293

Submission received: 4 March 2020 / Revised: 23 March 2020 / Accepted: 23 March 2020 / Published: 29 March 2020

Download

Browse Figures

Versions Notes

Abstract

Testosterone could have adverse effect on fertility. In this study, we hypothesized that this hormone could reduce the number of embryo implantations via affecting the normal endometrium ultrastructure and expression of endometrial proteins involved in implantation. Therefore, the aims were to identify these adverse testosterone effects. Methods: Intact pregnant rats were given 250 or 500 µg/kg/day testosterone for three days, beginning from day 1 of pregnancy. Rats were euthanized either at day 4 to analyze the ultra-structural changes in the endometrium and expression and distribution of MECA-79 protein, or at day 6 to determine the number of implantation sites. Results: Administration of 500 µg/kg/day testosterone suppresses endometrial pinopodes development and down-regulates expression and distribution of MECA-79 protein in the uterus. In addition, the number of implantation sites were markedly decreased. Conclusions: Changes in endometrial ultrastructure and expression of implantation protein in the endometrium in early pregnancy period could be the reason for failure of embryo implantation under testosterone influence.

Keywords: testosterone; uterine receptivity; embryo implantation

Share and Cite

MDPI and ACS Style

Mokhtar, M.H.; Giribabu, N.; Salleh, N. Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats. Int. J. Environ. Res. Public Health 2020, 17, 2293. https://doi.org/10.3390/ijerph17072293

AMA Style

Mokhtar MH, Giribabu N, Salleh N. Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats. International Journal of Environmental Research and Public Health. 2020; 17(7):2293. https://doi.org/10.3390/ijerph17072293

Chicago/Turabian Style

Mokhtar, Mohd Helmy, Nelli Giribabu, and Naguib Salleh. 2020. "Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats" International Journal of Environmental Research and Public Health 17, no. 7: 2293. https://doi.org/10.3390/ijerph17072293

APA Style

Mokhtar, M. H., Giribabu, N., & Salleh, N. (2020). Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats. International Journal of Environmental Research and Public Health, 17(7), 2293. https://doi.org/10.3390/ijerph17072293

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Testosterone Decreases the Number of Implanting Embryos, Expression of Pinopode and L-selectin Ligand (MECA-79) in the Endometrium of Early Pregnant Rats

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI