Transition Metal Containing Particulate Matter Promotes Th1 and Th17 Inflammatory Response by Monocyte Activation in Organic and Inorganic Compounds Dependent Manner
Abstract
:1. Introduction
2. Materials and Methods
2.1. TMCPM Preparation
2.2. Cell Isolation
2.3. Cell Culture and Immunostaining for Intracellular Proteins
2.4. Cytokine Detection by CBA
2.5. Endotoxin Determination
2.6. Statistics
3. Results
3.1. TMCPM Reduces the Expression Level of CD4 on the Surface of T Cells
3.2. TMCPM Activates CD4+ T Lymphocytes Depending on the Organic Compounds Content
3.3. TMCPM Exposure Is Skewing the Balance of Th1/Th2 and Treg/Th17 Subsets
3.4. Monocytes Activation Is Required for TMCPM Induced Polarization of Th1 and Th17 Immune Response
3.5. Endotoxin Content Is Partially Responsible for Pro-Inflammatory Potency of TMCPM
3.6. Both Organic and Inorganic Components of TMCPM Might Be Responsible for Activation of Monocytes and Propagation of Inflammatory Response
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample | LPS [EU/ml] | |||
---|---|---|---|---|
Polymyxin B 100 [µg/mL] | ||||
E. coli | [ng/mL] | LPS 0.5 | 5.50 | 0.00 |
TMCPM | [µg/mL] | NIST 100 | 1.75 | 0.10 |
LAP 100 | 0.40 | 0.00 |
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Gałuszka, A.; Stec, M.; Węglarczyk, K.; Kluczewska, A.; Siedlar, M.; Baran, J. Transition Metal Containing Particulate Matter Promotes Th1 and Th17 Inflammatory Response by Monocyte Activation in Organic and Inorganic Compounds Dependent Manner. Int. J. Environ. Res. Public Health 2020, 17, 1227. https://doi.org/10.3390/ijerph17041227
Gałuszka A, Stec M, Węglarczyk K, Kluczewska A, Siedlar M, Baran J. Transition Metal Containing Particulate Matter Promotes Th1 and Th17 Inflammatory Response by Monocyte Activation in Organic and Inorganic Compounds Dependent Manner. International Journal of Environmental Research and Public Health. 2020; 17(4):1227. https://doi.org/10.3390/ijerph17041227
Chicago/Turabian StyleGałuszka, Adrianna, Małgorzata Stec, Kazimierz Węglarczyk, Anna Kluczewska, Maciej Siedlar, and Jarek Baran. 2020. "Transition Metal Containing Particulate Matter Promotes Th1 and Th17 Inflammatory Response by Monocyte Activation in Organic and Inorganic Compounds Dependent Manner" International Journal of Environmental Research and Public Health 17, no. 4: 1227. https://doi.org/10.3390/ijerph17041227