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Open AccessBrief Report

Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study

1
Department of Dental and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy
2
Department of Surgical Sciences, Oral Biotechnology Laboratory, University of Cagliari, 09124 Cagliari, Italy
3
Department of Biomedical Sciences, University of Sassari, 07100 Sassari, Italy
4
Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Aldo Moro, 70122 Bari, Italy
5
National Research Council of Italy, ISPA-CNR, 07100 Sassari, Italy
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2020, 17(23), 9106; https://doi.org/10.3390/ijerph17239106
Received: 15 October 2020 / Revised: 3 December 2020 / Accepted: 4 December 2020 / Published: 6 December 2020
(This article belongs to the Special Issue Patient-Based Dentistry: Personalized Therapy and Prevention)
(1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against Streptococcus mutans. (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rose (IR) (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2- to 256-folds in saline solution, as to reproduce the salivary dilution. Streptococcus mutans CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby–Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate Streptococcus mutans avoiding the salivary dilution effect in the oral cavity. View Full-Text
Keywords: ozonated olive oil; S. mutans; mouthwash; caries ozonated olive oil; S. mutans; mouthwash; caries
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MDPI and ACS Style

Nardi, G.M.; Fais, S.; Casu, C.; Mazur, M.; Di Giorgio, R.; Grassi, R.; Grassi, F.R.; Orrù, G. Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study. Int. J. Environ. Res. Public Health 2020, 17, 9106. https://doi.org/10.3390/ijerph17239106

AMA Style

Nardi GM, Fais S, Casu C, Mazur M, Di Giorgio R, Grassi R, Grassi FR, Orrù G. Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study. International Journal of Environmental Research and Public Health. 2020; 17(23):9106. https://doi.org/10.3390/ijerph17239106

Chicago/Turabian Style

Nardi, Gianna M.; Fais, Sara; Casu, Cinzia; Mazur, Marta; Di Giorgio, Roberto; Grassi, Roberta; Grassi, Felice R.; Orrù, Germano. 2020. "Mouthwash Based on Ozonated Olive Oil in Caries Prevention: A Preliminary In-Vitro Study" Int. J. Environ. Res. Public Health 17, no. 23: 9106. https://doi.org/10.3390/ijerph17239106

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