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Open AccessArticle

Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats

by Ling Guo 1,2,†, Jia-Jin Lin 1,2,†, Yi-Zhe Xue 1, Guang-Zhou An 1,2, Jun-Ping Zhang 1, Ke-Ying Zhang 1, Wei He 1,2, Huan Wang 1,2, Wei Li 3,* and Gui-Rong Ding 1,2,*
1
Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
2
Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, 169# Chang Le West Road, Xi’an 710032, China
3
Department of Histology and Embryology, Fourth Military Medical University, 169# Chang Le West Road, Xi’an 710032, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the work.
Int. J. Environ. Res. Public Health 2019, 16(7), 1286; https://doi.org/10.3390/ijerph16071286
Received: 19 March 2019 / Revised: 1 April 2019 / Accepted: 4 April 2019 / Published: 10 April 2019
Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) fields. In this study, we investigated the effects of one-month exposure to a 220 MHz pulsed modulated RF field at the power density of 50 W/m2 on the sperm quality in male adult rats. The sperm quality was evaluated by measuring the number, abnormality and survival rate of sperm cells. The morphology of testis was examined by hematoxylin–eosin (HE) staining. The levels of secreting factors by Sertoli cells (SCs) and Leydig cells (LCs) were determined by enzyme linked immunosorbent assay (ELISA). The level of cleaved caspase 3 in the testis was detected by immunofluorescence staining. Finally, the expression levels of the apoptosis-related protein (caspase 3, BAX and BCL2) in the testis were assessed by Western blotting. Compared with the sham group, the sperm quality in the RF group decreased significantly. The levels of secreting factors of SCs and the morphology of the testis showed an obvious change after RF exposure. The level of the secreting factor of LCs decreased significantly after RF exposure. The levels of cleaved caspase 3, caspase 3, and the BAX/BCL2 ratio in the testis increased markedly after RF exposure. These data collectively suggested that under the present experimental conditions, 220 MHz pulsed modulated RF exposure could impair sperm quality in rats, and the disruption of the secreting function of LCs and increased apoptosis of testis cells induced by the RF field might be accounted for by this damaging effect. View Full-Text
Keywords: RF field; testis; sperm quality; apoptosis; rat RF field; testis; sperm quality; apoptosis; rat
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Guo, L.; Lin, J.-J.; Xue, Y.-Z.; An, G.-Z.; Zhang, J.-P.; Zhang, K.-Y.; He, W.; Wang, H.; Li, W.; Ding, G.-R. Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats. Int. J. Environ. Res. Public Health 2019, 16, 1286.

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