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Open AccessArticle

Levels of Urinary Biomarkers of Oxidatively Generated Damage to DNA and RNA in Different Groups of Workers Compared to General Population

1
Department of Occupational Medicine, Epidemiology, Occupational and Environmental Hygiene, INAIL Research, via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy
2
Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, via Giustiniani 2, 35128 Padova, Italy
3
CNR-ISAC—Italian National Research Council, Institute of Atmospheric Science and Climate, via Fosso del Cavaliere 100, 00133 Rome, Italy
4
Department of Epidemiology, Lazio Regional Health Service, Via Cristoforo Colombo 112, 00147 Rome, Italy
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2019, 16(16), 2995; https://doi.org/10.3390/ijerph16162995
Received: 19 July 2019 / Revised: 8 August 2019 / Accepted: 16 August 2019 / Published: 20 August 2019
(This article belongs to the Special Issue Human Biomonitoring of Environmental and Occupational Exposures)
(1) Background: The products of guanine oxidation in DNA and RNA excreted in urine are 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodGuo). Despite intra and inter-individual variability, it is possible to identify situations that significantly increase the levels of these compounds when comparing urinary concentrations of some workers to those of the general population. (2) Methods: urines from gasoline pump attendants (58 from Saudi Arabia and 102 from Italy), 24 workers of a fiberglass reinforced plastics plant, 17 painters and 6 divers were analyzed by HPLC/MS-MS. To test the individual variability, two subjects provided daily samples for one month, and 132 urine samples from the general population were analyzed. (3) Results: We summarized the results for each biomarker, and found the following were statistically higher than in the general population: 8-oxoGua in fiberglass and Italian gasoline workers; 8-oxodGuo in fiberglass and both Saudi Arabian and Italian gasoline workers; 8-oxoGuo in fiberglass workers, both Saudi Arabian and Italian gasoline workers, and painters after the working shift. (4) Conclusions: these results confirm that both 8-oxodGuo and 8-oxoGuo are valuable biomarkers for occupational exposures to dangerous chemicals and seem to suggest that 8-oxoGuo, related to RNA oxidation, is a suitable biomarker to evaluate short term, reversible effects of occupational exposures even within the health-based limit values. View Full-Text
Keywords: DNA and RNA oxidation; HPLC-MS/MS; biological monitoring; effect biomarkers; occupational exposure DNA and RNA oxidation; HPLC-MS/MS; biological monitoring; effect biomarkers; occupational exposure
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Tranfo, G.; Paci, E.; Carrieri, M.; Marchetti, E.; Sisto, R.; Gherardi, M.; Costabile, F.; Bauleo, L.; Ancona, C.; Pigini, D. Levels of Urinary Biomarkers of Oxidatively Generated Damage to DNA and RNA in Different Groups of Workers Compared to General Population. Int. J. Environ. Res. Public Health 2019, 16, 2995.

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