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Anaerobic Dechlorination by a Humin-Dependent Pentachlorophenol-Dechlorinating Consortium under Autotrophic Conditions Induced by Homoacetogenesis

1
Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan
2
National Institute for Land and Infrastructure Management, Tsukuba 305-0804, Japan
3
Institute of Materials and Systems for Sustainability, Nagoya University, Nagoya 464-8603, Japan
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2019, 16(16), 2873; https://doi.org/10.3390/ijerph16162873
Received: 21 July 2019 / Revised: 3 August 2019 / Accepted: 9 August 2019 / Published: 11 August 2019
(This article belongs to the Section Environmental Science and Engineering)
PDF [580 KB, uploaded 11 August 2019]

Abstract

Anoxic aquifers suffer from energy limitations due to the unavailability of organic substrates, as dictated by hydrogen (H2) for various electron-accepting processes. This deficiency often results in the accumulation of persistent organic pollutants, where bioremediation using organic compounds often leads to secondary contamination. This study involves the reductive dechlorination of pentachlorophenol (PCP) by dechlorinators that do not use H2 directly, but rather through a reduced state of humin—a solid-phase humic substance—as the extracellular electron donor, which requires an organic donor such as formate, lactate, etc. This shortcoming was addressed by the development of an anaerobic mixed culture that was capable of reductively dechlorinating PCP using humin under autotrophic conditions induced by homoacetogenesis. Here, H2 was used for carbon-dioxide fixation to acetate; the acetate produced was used for the reduction of humin; and consequently used for dechlorination through reduced humin. The 16SrRNA gene sequencing analysis showed Dehalobacter and Dehalobacterium as the possible dechlorinators, while Clostridium and Oxobacter were identified as the homoacetogens. Thus, this work contributes to the development of an anaerobic consortium that balanced H2 dependency, where efficiency of humin reduction extends the applicability of anaerobic microbial remediation in aquifers through autotrophy, syntrophy, and reductive dechlorination.
Keywords: autotrophic; H2; CO2; humin; acetogens; reductive dechlorination; homoacetogenesis autotrophic; H2; CO2; humin; acetogens; reductive dechlorination; homoacetogenesis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Laskar, M.; Awata, T.; Kasai, T.; Katayama, A. Anaerobic Dechlorination by a Humin-Dependent Pentachlorophenol-Dechlorinating Consortium under Autotrophic Conditions Induced by Homoacetogenesis. Int. J. Environ. Res. Public Health 2019, 16, 2873.

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