Metabolomics Reveals Metabolic Changes Caused by Low-Dose 4-Tert-Octylphenol in Mice Liver
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Animals and Treatments
2.3. Analysis of Serum Levels of ALT and AST
2.4. Histological Evaluations
2.5. Sample Preparation for the Mass Spectrometry Analysis of Mouse Liver and Blood Serum
2.6. Metabolomic Profiling
2.7. Targeted S-Adenosylmethionine Analysis in Liver and 4-Tert-Octylphenol in Blood Serum
2.8. Real-Time PCR Analysis
2.9. Enzyme-Linked Immunosorbent Assay
2.10. Statistical Analysis
3. Results
3.1. Changes of Body Weight and Liver Organ Coefficient
3.2. Histological Results
3.3. Results of the Serum ALT and AST
3.4. Metabolomic Profiles
3.5. Low-Dose OP Promoted Pyrimidine Synthesis through Cytidine Deaminase
3.6. Purine Synthesis Was Increased through Pyridoxal-5′-Phosphate (PLP)-Dependent Enzyme-Shmt1 after Low-Dose OP Treatment
3.7. Amino Acid Derivative Was Increased after Low-Dose OP Treatment
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Target Gene | GenBank Accession no. | Product Length (bp) | Primer Sequences |
---|---|---|---|
Cda | NM_028176.1 | 78 | Sense: 5′-ATGAGAGAGTTTGGCACCGAC-3′ |
Anti-sense:5′-CTCCTGGACCGTCCTGACTA-3′ | |||
Shmt1 | NM_009171.2 | 94 | Sense: 5′-CCCGAAACCAAGTGAACTGGA-3′ |
Anti-sense:5′-ACTGGTTCAGAGTTGCCTTGTA-3′ | |||
Gapdh | NM_001289726.1 | 124 | Sense: 5′-CCCTTAAGAGGGATGCTGCC-3′ |
Anti-sense:5′-TACGGCCAAATCCGTTCACA-3′ |
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Zhou, K.; Ding, X.; Yang, J.; Hu, Y.; Song, Y.; Chen, M.; Sun, R.; Dong, T.; Xu, B.; Han, X.; et al. Metabolomics Reveals Metabolic Changes Caused by Low-Dose 4-Tert-Octylphenol in Mice Liver. Int. J. Environ. Res. Public Health 2018, 15, 2686. https://doi.org/10.3390/ijerph15122686
Zhou K, Ding X, Yang J, Hu Y, Song Y, Chen M, Sun R, Dong T, Xu B, Han X, et al. Metabolomics Reveals Metabolic Changes Caused by Low-Dose 4-Tert-Octylphenol in Mice Liver. International Journal of Environmental Research and Public Health. 2018; 15(12):2686. https://doi.org/10.3390/ijerph15122686
Chicago/Turabian StyleZhou, Kun, Xingwang Ding, Jing Yang, Yanhui Hu, Yun Song, Minjian Chen, Rongli Sun, Tianyu Dong, Bo Xu, Xiumei Han, and et al. 2018. "Metabolomics Reveals Metabolic Changes Caused by Low-Dose 4-Tert-Octylphenol in Mice Liver" International Journal of Environmental Research and Public Health 15, no. 12: 2686. https://doi.org/10.3390/ijerph15122686