Low Dose Cadmium Inhibits Proliferation of Human Renal Mesangial Cells via Activation of the JNK Pathway
Abstract
:1. Introduction
2. Experimental Section
2.1. Cell Culture
2.2. Western Blotting
2.3. Cell Proliferation Assay
2.4. Cell Viability Assay
2.5. Annexin V-FITC/PI Analyses
2.6. Immunofluorescence
2.7. Phalloidin-Labelling
2.8. Statistical Analysis
3. Results
3.1. Low Dose Cd Activates JNK Pathway in HRMCs
3.2. Low Dose Cd Inhibits Proliferation of HRMC via Activation of JNK Pathway
3.3. Effects of Low Dose Cd Exposure on Apoptosis of HRMCs.
3.4. Effects of Low Dose Cd Exposure on theMesangial Cell Markers on HRMCs
3.5. Effects of Low Dose Cd Exposure on the Actin Cytoskeleton of HRMCs
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Chen, X.; Li, J.; Cheng, Z.; Xu, Y.; Wang, X.; Li, X.; Xu, D.; Kapron, C.M.; Liu, J. Low Dose Cadmium Inhibits Proliferation of Human Renal Mesangial Cells via Activation of the JNK Pathway. Int. J. Environ. Res. Public Health 2016, 13, 990. https://doi.org/10.3390/ijerph13100990
Chen X, Li J, Cheng Z, Xu Y, Wang X, Li X, Xu D, Kapron CM, Liu J. Low Dose Cadmium Inhibits Proliferation of Human Renal Mesangial Cells via Activation of the JNK Pathway. International Journal of Environmental Research and Public Health. 2016; 13(10):990. https://doi.org/10.3390/ijerph13100990
Chicago/Turabian StyleChen, Xiaocui, Jing Li, Zuowang Cheng, Yinghua Xu, Xia Wang, Xiaorui Li, Dongmei Xu, Carolyn M. Kapron, and Ju Liu. 2016. "Low Dose Cadmium Inhibits Proliferation of Human Renal Mesangial Cells via Activation of the JNK Pathway" International Journal of Environmental Research and Public Health 13, no. 10: 990. https://doi.org/10.3390/ijerph13100990