Effects and Interactions of Medium Components on Laccase from a Marine-Derived Fungus Using Response Surface Methodology
1
Marine Biotechnology Laboratory, National Institute of Oceanography, Council of Scientific & Industrial Research, Dona Paula, Goa 403 004, India
2
Department of Microbiology, Goa University, Taleigao Plateau, Goa 403 206, India
*
Author to whom correspondence should be addressed.
Mar. Drugs 2009, 7(4), 672-688; https://doi.org/10.3390/md7040672
Received: 8 October 2009 / Revised: 30 October 2009 / Accepted: 2 November 2009 / Published: 25 November 2009
(This article belongs to the Special Issue Enzymes from the Sea: Sources, Molecular Biology and Bioprocesses)
The effects of various synthetic medium components and their interactions with each other ultimately impact laccase production in fungi. This was studied using a laccasehyper-producing marine-derived basidiomycete, Cerrena unicolor MTCC 5159. Inducible laccases were produced in the idiophase only after addition of an inducer such as CuSO4. Concentration of carbon and nitrogen acted antagonistically with respect to laccase production. A combination of low nitrogen and high carbon concentration favored both biomass and laccase production. The most favorable combination resulted in 917 U L-1 of laccase. After sufficient growth had occurred, addition of a surfactant such as Tween 80 positively impacted biomass and increased the laccase activity to around 1,300 U L-1. Increasing the surface to volume ratio of the culture vessel further increased its activity to almost 2,000 U L-1.
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Keywords:
marine-derived basidiomycete; laccase; response surface methodology; Central Composite Design and Plackett Burman Design
This is an open access article distributed under the Creative Commons Attribution License
MDPI and ACS Style
D’Souza-Ticlo, D.; Garg, S.; Raghukumar, C. Effects and Interactions of Medium Components on Laccase from a Marine-Derived Fungus Using Response Surface Methodology. Mar. Drugs 2009, 7, 672-688. https://doi.org/10.3390/md7040672
AMA Style
D’Souza-Ticlo D, Garg S, Raghukumar C. Effects and Interactions of Medium Components on Laccase from a Marine-Derived Fungus Using Response Surface Methodology. Marine Drugs. 2009; 7(4):672-688. https://doi.org/10.3390/md7040672
Chicago/Turabian StyleD’Souza-Ticlo, Donna; Garg, Sandeep; Raghukumar, Chandralata. 2009. "Effects and Interactions of Medium Components on Laccase from a Marine-Derived Fungus Using Response Surface Methodology" Mar. Drugs 7, no. 4: 672-688. https://doi.org/10.3390/md7040672
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