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Article

Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium

1
Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Minamitamagaki-cho 3500-3, Suzuka 513-8670, Mie, Japan
2
Konan Chemical Manufacturing, Co., Ltd., 1515 Kitagomizuka, Yokkaichi 510-0103, Mie, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Anna Palumbo
Mar. Drugs 2022, 20(2), 121; https://doi.org/10.3390/md20020121
Received: 17 January 2022 / Revised: 31 January 2022 / Accepted: 1 February 2022 / Published: 3 February 2022
(This article belongs to the Special Issue Sulfur-Containing Marine Bioactives 2.0)
We previously reported that rhamnan sulfate (RS) purified from Monostroma nitidum significantly suppressed lipopolysaccharide (LPS)-induced inflammation in cultured human vascular endothelial cells. Here, we analyzed the effect of orally administered RS on LPS-induced damage to mouse organs and vascular endothelium. RS (1 mg) was orally administered daily to BALB/c mice, 50 μg of LPS was intraperitoneally administered on day 8, and Evans blue was injected into the tail vein 6 h later. After 30 min, LPS-treated mice showed pulmonary Evans blue leakage and elevated plasma levels of liver damage markers, whereas this reaction was suppressed in LPS + RS-treated mice. Immunohistochemical and Western blot analysis of mouse organs 24 h after LPS treatment showed significant neutrophil infiltration into the lung, liver, and jejunum tissues of LPS-treated mice and high expression levels of inflammation-related factors in these tissues. Expression levels of these factors were significantly suppressed in LPS + RS-treated mice. Analysis of lung glycocalyx showed a significant reduction in glycocalyx in LPS-treated mice but not in LPS + RS-treated mice. Levels of syndecan-4, one of the glycocalyx components, decreased in LPS-treated mice and increased in LPS + RS-treated mice. The current results suggest that orally administered RS protects organs and vascular endothelium from LPS-induced inflammation and maintains blood circulation. View Full-Text
Keywords: Monostroma nitidum; rhamnan sulfate; endothelium; anti-inflammation; glycocalyx Monostroma nitidum; rhamnan sulfate; endothelium; anti-inflammation; glycocalyx
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MDPI and ACS Style

Terasawa, M.; Hiramoto, K.; Uchida, R.; Suzuki, K. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium. Mar. Drugs 2022, 20, 121. https://doi.org/10.3390/md20020121

AMA Style

Terasawa M, Hiramoto K, Uchida R, Suzuki K. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium. Marine Drugs. 2022; 20(2):121. https://doi.org/10.3390/md20020121

Chicago/Turabian Style

Terasawa, Masahiro, Keiichi Hiramoto, Ryota Uchida, and Koji Suzuki. 2022. "Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium" Marine Drugs 20, no. 2: 121. https://doi.org/10.3390/md20020121

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