Transcriptomic and Proteomic Characterizations of the Molecular Response to Blue Light and Salicylic Acid in Haematococcus pluvialis
Abstract
:1. Introduction
2. Results
2.1. Transcriptome Analysis
2.2. Identification and Enrichment Analysis of DEGs and DEPs
2.3. DEGs and DEPs Involved in the Astaxanthin Metabolic Pathway
2.4. DEGs and DEPs Associated with the Lipid Metabolic Pathway
2.5. DEGs and DEPs Associated with the Heme Pathway
2.6. DEGs and DEPs Associated with the Generation of ROS
2.7. DEGs and DEPs Associated with the Cell Wall Biosynthesis
2.8. DEGs Associated with the Blue Light Receptors
2.9. Identification of Transcription Factors
3. Discussion
3.1. Synthesis of Astaxanthin and Fatty Acids under Blue Light Irradiation
3.2. Scavenging of Reactive Oxygen Species under Blue Light Irradiation
3.3. Cell Wall Synthesis Inhibited in the Early Stage of the Treatment with Blue light Irradiation
3.4. Signal Transduction of Blue Light Receptors
4. Materials and Methods
4.1. Algal Materials
4.2. Transcriptome Sequencing
4.3. De Novo Assembly and Unigene Annotation
4.4. Differentially Expressed Unigenes Analysis
4.5. Proteome Sampling, Sequencing, and Bioinformatics
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Class | Unigene ID | B02 vs. W02 | B07 vs. W07 | B02 vs. N | B07 vs. B02 | B07 vs. N | B252 vs. B02 | B257 vs. B07 |
---|---|---|---|---|---|---|---|---|
dashCRY | N40695_c3_g2 | −0.47 | −0.12 | −0.54 | −0.39 | −0.70 | −0.17 | 0.38 |
dashCRY | N43038_c0_g10 | −0.74 | −0.49 | −1.48 | −0.09 | −1.34 | 0.48 | 0.45 |
dashCRY | N49636_c1_g1 | 0.35 | −0.33 | −4.15 | −1.54 | −5.46 | 0.03 | 1.01 |
PHOT | N44442_c1_g2 | −0.15 | −0.03 | 0.32 | −0.48 | 0.07 | 0.30 | −0.12 |
PHOT | N50230_c0_g1 | −0.32 | −0.34 | −0.93 | −0.68 | −1.38 | 0.14 | 0.02 |
CRY | N44179_c1_g4 | 0.00 | −0.41 | −1.40 | −0.65 | −1.83 | 0.16 | −0.03 |
CRY | N50367_c0_g2 | 0.21 | −0.11 | −1.58 | −0.97 | −2.33 | −0.20 | 0.06 |
Transcription Factor | B02 vs. W02 | B07 vs. W07 | B257 vs. B07 |
---|---|---|---|
C2H2 | 45 | 30 | 44 |
C3H | 20 | 20 | 16 |
GNAT | – | 13 | 13 |
HMG | 17 | 12 | 16 |
Jumonji | 13 | 10 | – |
MYB | 21 | – | 16 |
MYB-related | 18 | 12 | 21 |
SET | 13 | 17 | 16 |
SNF2 | 21 | 41 | 38 |
TRAF | 38 | 57 | 50 |
zn-clus | 31 | 43 | 40 |
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Wang, X.; Meng, C.; Zhang, H.; Xing, W.; Cao, K.; Zhu, B.; Zhang, C.; Sun, F.; Gao, Z. Transcriptomic and Proteomic Characterizations of the Molecular Response to Blue Light and Salicylic Acid in Haematococcus pluvialis. Mar. Drugs 2022, 20, 1. https://doi.org/10.3390/md20010001
Wang X, Meng C, Zhang H, Xing W, Cao K, Zhu B, Zhang C, Sun F, Gao Z. Transcriptomic and Proteomic Characterizations of the Molecular Response to Blue Light and Salicylic Acid in Haematococcus pluvialis. Marine Drugs. 2022; 20(1):1. https://doi.org/10.3390/md20010001
Chicago/Turabian StyleWang, Xiaodong, Chunxiao Meng, Hao Zhang, Wei Xing, Kai Cao, Bingkui Zhu, Chengsong Zhang, Fengjie Sun, and Zhengquan Gao. 2022. "Transcriptomic and Proteomic Characterizations of the Molecular Response to Blue Light and Salicylic Acid in Haematococcus pluvialis" Marine Drugs 20, no. 1: 1. https://doi.org/10.3390/md20010001