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Article

Characterization of Neoagarooligosaccharide Hydrolase BpGH117 from a Human Gut Bacterium Bacteroides plebeius

Department of Biotechnology, Graduate School, Korea University, Seoul 02841, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Herminia Domínguez, María Lourdes Mourelle and Jose Luis Legido
Mar. Drugs 2021, 19(5), 271; https://doi.org/10.3390/md19050271
Received: 1 March 2021 / Revised: 10 May 2021 / Accepted: 10 May 2021 / Published: 13 May 2021
(This article belongs to the Special Issue Nutra-Cosmeceuticals from Algae for Health and Wellness)
α-Neoagarobiose (NAB)/neoagarooligosaccharide (NAO) hydrolase plays an important role as an exo-acting 3,6-anhydro-α-(1,3)-L-galactosidase in agarose utilization. Agarose is an abundant polysaccharide found in red seaweeds, comprising 3,6-anhydro-L-galactose (AHG) and D-galactose residues. Unlike agarose degradation, which has been reported in marine microbes, recent metagenomic analysis of Bacteroides plebeius, a human gut bacterium, revealed the presence of genes encoding enzymes involved in agarose degradation, including α-NAB/NAO hydrolase. Among the agarolytic enzymes, BpGH117 has been partially characterized. Here, we characterized the exo-acting α-NAB/NAO hydrolase BpGH117, originating from B. plebeius. The optimal temperature and pH for His-tagged BpGH117 activity were 35 °C and 9.0, respectively, indicative of its unique origin. His-tagged BpGH117 was thermostable up to 35 °C, and the enzyme activity was maintained at 80% of the initial activity at a pre-incubation temperature of 40 °C for 120 min. Km and Vmax values for NAB were 30.22 mM and 54.84 U/mg, respectively, and kcat/Km was 2.65 s−1 mM−1. These results suggest that His-tagged BpGH117 can be used for producing bioactive products such as AHG and agarotriose from agarose efficiently. View Full-Text
Keywords: α-neoagarooligosaccharide hydrolase; exo-acting 3,6-anhydro-α-(1,3)-L-galactosidase; BpGH117; 3,6-anhydro-L-galactose; human gut bacterium; Bacteroides plebeius; agarose α-neoagarooligosaccharide hydrolase; exo-acting 3,6-anhydro-α-(1,3)-L-galactosidase; BpGH117; 3,6-anhydro-L-galactose; human gut bacterium; Bacteroides plebeius; agarose
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MDPI and ACS Style

Jin, Y.; Yu, S.; Kim, D.H.; Yun, E.J.; Kim, K.H. Characterization of Neoagarooligosaccharide Hydrolase BpGH117 from a Human Gut Bacterium Bacteroides plebeius. Mar. Drugs 2021, 19, 271. https://doi.org/10.3390/md19050271

AMA Style

Jin Y, Yu S, Kim DH, Yun EJ, Kim KH. Characterization of Neoagarooligosaccharide Hydrolase BpGH117 from a Human Gut Bacterium Bacteroides plebeius. Marine Drugs. 2021; 19(5):271. https://doi.org/10.3390/md19050271

Chicago/Turabian Style

Jin, Yerin, Sora Yu, Dong H. Kim, Eun J. Yun, and Kyoung H. Kim 2021. "Characterization of Neoagarooligosaccharide Hydrolase BpGH117 from a Human Gut Bacterium Bacteroides plebeius" Marine Drugs 19, no. 5: 271. https://doi.org/10.3390/md19050271

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