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3-D Culture of Marine Sponge Cells for Production of Bioactive Compounds

Harbor Branch Oceanographic Institute, Florida Atlantic University, 5600 US Highway 1 North, Fort Pierce, FL 34946, USA
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Academic Editors: Micha Ilan, Shmuel Carmeli, Michelle Kelly and Mark T. Hamann
Mar. Drugs 2021, 19(10), 569; https://doi.org/10.3390/md19100569
Received: 16 September 2021 / Revised: 5 October 2021 / Accepted: 9 October 2021 / Published: 14 October 2021
(This article belongs to the Special Issue Marine Sponge Biotechnology)
Production of sponge-derived bioactive compounds in vitro has been proposed as an alternative to wild harvest, aquaculture, and chemical synthesis to meet the demands of clinical drug development and manufacture. Until recently, this was not possible because there were no marine invertebrate cell lines. Recent breakthroughs in the development of sponge cell lines and rapid cell division in improved nutrient media now make this approach a viable option. We hypothesized that three-dimensional (3-D) cell cultures would better represent how sponges function in nature, including the production of bioactive compounds. We successfully cultured sponge cells in 3-D matrices using FibraCel® disks, thin hydrogel layers, and gel microdroplets (GMDs). For in vitro production of bioactive compounds, the use of GMDs is recommended. Nutrients and sponge products rapidly diffuse into and out of the 3-D matrix, the GMDs may be scaled up in spinner flasks, and cells and/or secreted products can be easily recovered. Research on scale-up and production is in progress in our laboratory. View Full-Text
Keywords: marine sponge; 3-D culture; FibraCel® disks; hydrogel; ultra low temperature agarose; gel microdroplets; marine natural products marine sponge; 3-D culture; FibraCel® disks; hydrogel; ultra low temperature agarose; gel microdroplets; marine natural products
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MDPI and ACS Style

Urban-Gedamke, E.; Conkling, M.; McCarthy, P.J.; Wills, P.S.; Pomponi, S.A. 3-D Culture of Marine Sponge Cells for Production of Bioactive Compounds. Mar. Drugs 2021, 19, 569. https://doi.org/10.3390/md19100569

AMA Style

Urban-Gedamke E, Conkling M, McCarthy PJ, Wills PS, Pomponi SA. 3-D Culture of Marine Sponge Cells for Production of Bioactive Compounds. Marine Drugs. 2021; 19(10):569. https://doi.org/10.3390/md19100569

Chicago/Turabian Style

Urban-Gedamke, Elizabeth, Megan Conkling, Peter J. McCarthy, Paul S. Wills, and Shirley A. Pomponi 2021. "3-D Culture of Marine Sponge Cells for Production of Bioactive Compounds" Marine Drugs 19, no. 10: 569. https://doi.org/10.3390/md19100569

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