Protective Effect of a Fucose-Rich Fucoidan Isolated from Saccharina japonica against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish
College of Food Science & Engineering, Ocean University of China, 5th Yushan Road, Qingdao 266003, China
Department of Marine Life Sciences, Jeju National University, Jeju Self-Governing Province 63243, Korea
Marine Science Institute, Jeju National University, Jeju Self-Governing Province 63333, Korea
State Key Lab of Seaweed Bioactive Substances, 1th Daxueyuan Road, Qingdao 266400, China
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, China
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(6), 316; https://doi.org/10.3390/md18060316
Received: 14 May 2020 / Revised: 30 May 2020 / Accepted: 8 June 2020 / Published: 15 June 2020
(This article belongs to the Special Issue Fucoidans)
A fucose-rich fucoidan was purified from brown seaweed Saccharina japonica, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated HaCaT cells were determined. The results indicate that the purified fucoidan significantly reduced the intracellular reactive oxygen species levels and improved the viability of UVB-irradiated HaCaT cells. Furthermore, the purified fucoidan remarkably decreased the apoptosis by regulating the expressions of Bax/Bcl-xL and cleaved caspase-3 in UVB-irradiated HaCaT cells in a dose-dependent manner. In addition, the in vivo UV protective effect of the purified fucoidan was investigated using a zebrafish model. It significantly reduced the intracellular reactive oxygen species level, the cell death, the NO production, and the lipid peroxidation in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that purified fucoidan has a great potential to be developed as a natural anti-UVB agent applied in the cosmetic industry.