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Open AccessArticle

Biochemical and Genomic Characterization of the Cypermethrin-Degrading and Biosurfactant-Producing Bacterial Strains Isolated from Marine Sediments of the Chilean Northern Patagonia

1
Laboratorio de Microbiología Molecular, Escuela de Tecnología Médica, Universidad Austral de Chile, Puerto Montt 5504335, Chile
2
Laboratorio de Bioinformática y Expresión Génica, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago 7810000, Chile
3
Center for Genome Regulation, Santiago 7810000, Chile
4
Laboratorio de Biología de Sistemas de Plantas, Departamento Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile
5
Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5090000, Chile
6
Laboratorio de Microbiología Ambiental y extremófilos, Departamento de Ciencias Biológicas y Biodiversidad, Universidad de los Lagos, Osorno 5290000, Chile
7
Escuela de Ingeniería Civil Industrial, Universidad Austral de Chile, Puerto Montt 5500000, Chile
8
Laboratorio Medicina Traslacional, Instituto Clínico Oncológico, Fundación Arturo López Pérez, Santiago 8320000, Chile
*
Authors to whom correspondence should be addressed.
These authors have contributed equally to this work.
Mar. Drugs 2020, 18(5), 252; https://doi.org/10.3390/md18050252
Received: 15 April 2020 / Revised: 9 May 2020 / Accepted: 11 May 2020 / Published: 13 May 2020
(This article belongs to the Special Issue Bioactive Molecules from Extreme Environments)
Pesticides cause severe environmental damage to marine ecosystems. In the last ten years, cypermethrin has been extensively used as an antiparasitic pesticide in the salmon farming industry located in Northern Patagonia. The objective of this study was the biochemical and genomic characterization of cypermethrin-degrading and biosurfactant-producing bacterial strains isolated from cypermethrin-contaminated marine sediment samples collected in southern Chile (MS). Eleven strains were isolated by cypermethrin enrichment culture techniques and were identified by 16S rDNA gene sequencing analyses. The highest growth rate on cypermethrin was observed in four isolates (MS13, MS15a, MS16, and MS19) that also exhibited high levels of biosurfactant production. Genome sequence analyses of these isolates revealed the presence of genes encoding components of bacterial secondary metabolism, and the enzymes esterase, pyrethroid hydrolase, and laccase, which have been associated with different biodegradation pathways of cypermethrin. These novel cypermethrin-degrading and biosurfactant-producing bacterial isolates have a biotechnological potential for biodegradation of cypermethrin-contaminated marine sediments, and their genomes contribute to the understanding of microbial lifestyles in these extreme environments. View Full-Text
Keywords: cypermethrin; biosurfactants; biodegradation capacities; marine sediments cypermethrin; biosurfactants; biodegradation capacities; marine sediments
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MDPI and ACS Style

Aguila-Torres, P.; Maldonado, J.; Gaete, A.; Figueroa, J.; González, A.; Miranda, R.; González-Stegmaier, R.; Martin, C.; González, M. Biochemical and Genomic Characterization of the Cypermethrin-Degrading and Biosurfactant-Producing Bacterial Strains Isolated from Marine Sediments of the Chilean Northern Patagonia. Mar. Drugs 2020, 18, 252.

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