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Open AccessArticle

A Marine Collagen-Based Biomimetic Hydrogel Recapitulates Cancer Stem Cell Niche and Enhances Progression and Chemoresistance in Human Ovarian Cancer

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Department of Obstetrics and Gynecology, Pusan National University College of Medicine, Yangsan, Gyeongsangnam-do 626-870, Korea
2
Department of Anatomy, Pusan National University College of Medicine, Yangsan, Gyeongsangnam-do 626-870, Korea
3
Immune Reconstitution Research Center of Medical Research Institute, Pusan National University College of Medicine, Yangsan, Gyeongsangnam-do 626-870, Korea
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(10), 498; https://doi.org/10.3390/md18100498
Received: 31 August 2020 / Revised: 25 September 2020 / Accepted: 28 September 2020 / Published: 29 September 2020
(This article belongs to the Special Issue Marine Collagen: A Promising Biomaterial for Biomedical Applications)
Recent attention has focused on the development of an effective three-dimensional (3D) cell culture system enabling the rapid enrichment of cancer stem cells (CSCs) that are resistant to therapies and serving as a useful in vitro tumor model that accurately reflects in vivo behaviors of cancer cells. Presently, an effective 3D in vitro model of ovarian cancer (OC) was developed using a marine collagen-based hydrogel. Advantages of the model include simplicity, efficiency, bioactivity, and low cost. Remarkably, OC cells grown in this hydrogel exhibited biochemical and physiological features, including (1) enhanced cell proliferation, migration and invasion, colony formation, and chemoresistance; (2) suppressed apoptosis with altered expression levels of apoptosis-regulating molecules; (3) upregulated expression of crucial multidrug resistance-related genes; (4) accentuated expression of key molecules associated with malignant progression, such as epithelial–mesenchymal transition transcription factors, Notch, and pluripotency biomarkers; and (5) robust enrichment of ovarian CSCs. The findings indicate the potential of our 3D in vitro OC model as an in vitro research platform to study OC and ovarian CSC biology and to screen novel therapies targeting OC and ovarian CSCs. View Full-Text
Keywords: marine collagen; hydrogel; cancer stem cell; ovarian cancer; 3D cell culture; spheroid; chemoresistance marine collagen; hydrogel; cancer stem cell; ovarian cancer; 3D cell culture; spheroid; chemoresistance
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Moon, S.; Ok, Y.; Hwang, S.; Lim, Y.S.; Kim, H.-Y.; Na, Y.-J.; Yoon, S. A Marine Collagen-Based Biomimetic Hydrogel Recapitulates Cancer Stem Cell Niche and Enhances Progression and Chemoresistance in Human Ovarian Cancer. Mar. Drugs 2020, 18, 498.

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