Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Ultraviolet (UV) Absorption Spectrum
2.2. Fourier Transform Infrared Spectroscopy (FTIR)
2.3. Amino Acid Analysis
2.4. Zeta Potential
2.5. Antioxidant Activity
2.6. Protective Effect against H2O2-induced Injury on RAW264.7 Macrophage Cells
3. Materials and Methods
3.1. Materials
3.2. Extraction of Collagen from A. Molpadioides
3.3. UV Absorption Spectrum
3.4. FTIR Spectrum Analysis
3.5. Amino Acid Analysis
3.6. Zeta Potential
3.7. Antioxidant Activity
3.7.1. DPPH Radical Scavenging Activity
3.7.2. ABTS Radical Scavenging Activity
3.8. Cell Morphology Observation and Measurement of Cell Viability
3.9. Assays for the Levels of MDA, SOD, and GSH-Px
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Amino Acid | A. Molpadioides | S. Monotuberculatus | H. Parva | Calf Skin |
---|---|---|---|---|
Aspartic acid (Asp) | 63.7 | 70 | 50 | 45 |
Threonine (Thr) | 40.8 | 34 | ND 1 | ND 1 |
Serine (Ser) | 40.7 | 26 | 20 | 39 |
Glutamic acid (Glu) | 100.8 | 127 | 74 | 75 |
Glycine (Gly) | 336.9 | 320 | 270 | 330 |
Alanine (Ala) | 127.9 | 92 | 91 | 119 |
Valine (Val) | 21.1 | 21 | 18 | 21 |
Methionine (Met) | 5.7 | 6 | 5 | 6 |
Isoleucine (Ile) | 7.1 | 15 | 4 | 11 |
Leucine (Leu) | 19.6 | 20 | 16 | 23 |
Tyrosine (Tyr) | 10.5 | 6 | 4 | 3 |
Phenylalanine (Phe) | 8.0 | 10 | 6 | 3 |
Lysine (Lys) | 4.1 | 6 | 7 | 26 |
Histidine (His) | 4.6 | 9 | ND 1 | 5 |
Arginine (Arg) | 45.1 | 71 | 49 | 50 |
Proline (Pro) | 98.5 | 84 | 96 | 121 |
Hydroxyproline (Hyp) | 57.5 | 67 | 62 | 94 |
Imino Acid | 156 | 151 | 158 | 215 |
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Li, J.; Li, Y.; Li, Y.; Yang, Z.; Jin, H. Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells. Mar. Drugs 2020, 18, 370. https://doi.org/10.3390/md18070370
Li J, Li Y, Li Y, Yang Z, Jin H. Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells. Marine Drugs. 2020; 18(7):370. https://doi.org/10.3390/md18070370
Chicago/Turabian StyleLi, Jie, Yan Li, Yuyao Li, Zuisu Yang, and Huoxi Jin. 2020. "Physicochemical Properties of Collagen from Acaudina Molpadioides and Its Protective Effects against H2O2-Induced Injury in RAW264.7 Cells" Marine Drugs 18, no. 7: 370. https://doi.org/10.3390/md18070370