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Open AccessArticle

Ubiquitin-Proteasome Modulating Dolabellanes and Secosteroids from Soft Coral Clavularia flava

1
Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 80441, Taiwan
2
Department of Microbiology and Immunology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2020, 18(1), 39; https://doi.org/10.3390/md18010039
Received: 10 December 2019 / Revised: 27 December 2019 / Accepted: 30 December 2019 / Published: 3 January 2020
(This article belongs to the Special Issue Selected Papers from XVI MaNaPro and XI ECMNP)
We performed a high-content screening (HCS) assay aiming to discover bioactive molecules with proteasome inhibitory activity. By structural elucidation, we identified six compounds purified from soft coral Clavularia flava, which potentiates proteasome inhibition. Chemical structure elucidation revealed they are dolabellane- and secosteroid-based compounds including a new dolabellane, clavinflol C (1), three known dolabellanes, stolonidiol (2), stolonidiol-17-acetate (3), and clavinflol B (4) as well as two new secosteroids, 3β,11-dihydroxy-24-methyl-9,11-secocholest-5-en-9,23-dione (5) and 3β,11-dihydroxy-24-methylene-9,11-secocholest-5-en-9,23-dione (6). All six compounds show less cytotoxicity than those of known proteasome inhibitors, bortezomib and MG132. In summary, the high-content measurements of control inhibitors, bortezomib and MG132, manifest the highest ratio >2 in high-content measurement. Of the isolated compounds, 2 and 5 showed higher activity, followed by 3 and 6, and then 1 and 4 exhibited moderate inhibition. View Full-Text
Keywords: proteasome inhibition; dolabellane; secosteroids; soft coral proteasome inhibition; dolabellane; secosteroids; soft coral
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MDPI and ACS Style

Chiu, C.-Y.; Ling, X.-H.; Wang, S.-K.; Duh, C.-Y. Ubiquitin-Proteasome Modulating Dolabellanes and Secosteroids from Soft Coral Clavularia flava. Mar. Drugs 2020, 18, 39.

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