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Recombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp

1
Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
2
Department of Microbiology, Nanjing Institute of Fisheries Science, Nanjing 210036, China
3
Department of Biology, Texas State University, San Marcos, TX 78666, USA
*
Author to whom correspondence should be addressed.
Bao Zhang and Xiyi Zhuang contributed equally to this work.
Mar. Drugs 2019, 17(9), 499; https://doi.org/10.3390/md17090499
Received: 26 June 2019 / Revised: 15 August 2019 / Accepted: 24 August 2019 / Published: 27 August 2019
Quorum quenching (QQ) is a promising alternative infection-control strategy to antibiotics that controls quorum-regulated virulence without killing the pathogens. Aeromonas hydrophila is an opportunistic gram-negative pathogen living in freshwater and marine environments. A. hydrophila possesses an N-acyl homoserine lactone (AHL)-based quorum-sensing (QS) system that regulates virulence, so quorum signal-inactivation (i.e., QQ) may represent a new way to combat A. hydrophila infection. In this study, an AHL lactonase gene, aiiA was cloned from Bacillus sp. strain QSI-1 and expressed in Escherichia coli strain BL21(DE3). The A. hydrophila hexanoyl homoserine lactone (C6-HSL) QS signal molecule was degraded by AiiAQSI-1, which resulted in a decrease of bacterial swimming motility, reduction of extracellular protease and hemolysin virulence factors, and inhibited the biofilm formation of A. hydrophila YJ-1 in a microtiter assay. In cell culture studies, AiiAQSI-1 decreased the ability of A. hydrophila adherence to and internalization by Epithelioma papulosum cyprini (EPC) cells. During in vivo studies, oral administration of AiiAQSI-1 via feed supplementation attenuated A. hydrophila infection in Crucian Carp. Results from this work indicate that feed supplementation with AiiAQSI-1 protein has potential to control A. hydrophila aquaculture disease via QQ. View Full-Text
Keywords: N-acyl homoserine lactone lactonase; Aeromonas hydrophila; Crucian Carp; virulence factors; biofilm N-acyl homoserine lactone lactonase; Aeromonas hydrophila; Crucian Carp; virulence factors; biofilm
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Zhang, B.; Zhuang, X.; Guo, L.; McLean, R.J.C.; Chu, W. Recombinant N-acyl homoserine lactone-Lactonase AiiAQSI-1 Attenuates Aeromonas hydrophila Virulence Factors, Biofilm Formation and Reduces Mortality in Crucian Carp. Mar. Drugs 2019, 17, 499.

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