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Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway

1
Convergence Stem Cell Research Center, Pusan National University, Yangsan 50612, Korea
2
Laboratory for Vascular Medicine and Stem Cell Biology, Department of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Korea
3
Department of Obstetrics and Gynecology, Biomedical Research Institute, Pusan National University School of Medicine, Busan 46241, Korea
4
Department of Microbiology and Immunology, Pusan National University School of Medicine, Yangsan 50612, Korea
5
Research Institute of Convergence Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan 50612, Korea
*
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(7), 392; https://doi.org/10.3390/md17070392
Received: 20 May 2019 / Revised: 26 June 2019 / Accepted: 28 June 2019 / Published: 3 July 2019
(This article belongs to the Special Issue Marine Natural Products and Cardiovascular Disease Prevention)
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Abstract

The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD. View Full-Text
Keywords: endothelial progenitor cells; cell proliferation; fucoidan; amyloglucosidase; vascular regeneration endothelial progenitor cells; cell proliferation; fucoidan; amyloglucosidase; vascular regeneration
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Rethineswaran, V.K.; Kim, Y.-J.; Jang, W.B.; Ji, S.T.; Kang, S.; Kim, D.Y.; Park, J.H.; Van, L.T.H.; Giang, L.T.T.; Ha, J.S.; Yun, J.; Lee, D.H.; Yu, S.-N.; Park, S.-G.; Ahn, S.-C.; Kwon, S.-M. Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway. Mar. Drugs 2019, 17, 392.

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