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Effect of Cyclophilin from Pyropia Yezoensis on the Proliferation of Intestinal Epithelial Cells by Epidermal Growth Factor Receptor/Ras Signaling Pathway

1
Department of Food Science and Nutrition, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan 48513, Korea
2
Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Korea
3
Department of Marine Bio-material & Aquaculture, Pukyong National University, 45, Yongso-ro, Nam-Gu, Busan 48513, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2019, 17(5), 297; https://doi.org/10.3390/md17050297
Received: 11 April 2019 / Revised: 9 May 2019 / Accepted: 15 May 2019 / Published: 18 May 2019
Cyclophilin (Cyp) is peptidyl–prolyl isomerase (PPIase), and it has many biological functions, including immune response regulation, antioxidants, etc. Cyp from red algae is known for its antioxidant and antifungal activity. However, the other biological effects of Cyp from Pyropia yezoensis are unclear. In this study, we synthesized Cyp from P. yezoensis (pyCyp) and examined its biological activity on IEC-6 cells. First, the MTS assay showed that pyCyp increased cell proliferation in a dose-dependent manner. pyCyp activated the EGFR signaling pathway that regulates cell growth, proliferation, and survival. It induced intracellular signaling pathways, including the Ras signaling pathway. In addition, we observed cell cycle-related proteins. pyCyp increased the expression of cyclin A, cyclin E, and Cdk2, and decreased the expression of p27 and p21 proteins. These results indicate that pyCyp stimulates cell proliferation via the EGFR signaling pathway and promotes cell cycle progression in intestinal epithelial cells. Therefore, we suggest pyCyp as a potential material to promote the proliferation of intestinal epithelial cells. View Full-Text
Keywords: Pyropia yezoensis; cyclohphilin; epidermal growth factor receptor; cell proliferation; recombinant protein Pyropia yezoensis; cyclohphilin; epidermal growth factor receptor; cell proliferation; recombinant protein
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Jung, J.-H.; Choi, J.-W.; Lee, M.-K.; Choi, Y.-H.; Nam, T.-J. Effect of Cyclophilin from Pyropia Yezoensis on the Proliferation of Intestinal Epithelial Cells by Epidermal Growth Factor Receptor/Ras Signaling Pathway. Mar. Drugs 2019, 17, 297.

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