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Mar. Drugs 2019, 17(4), 196; https://doi.org/10.3390/md17040196

Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by Vibrio diabolicus A1SM3

1
Bioscience Doctoral Program, Grupo de Investigación en Bioprospección, Faculty of Engineering, Universidad de La Sabana, Campus Puente del Común, Km 7, Autopista Norte de Bogotá, 250001 Chía, Colombia
2
Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto/SP, Brazil
3
Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo-SP, Brazil
*
Author to whom correspondence should be addressed.
Received: 1 March 2019 / Revised: 19 March 2019 / Accepted: 25 March 2019 / Published: 27 March 2019
(This article belongs to the Special Issue Bioprospecting of Marine Microorganisms)
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Abstract

Vibrio diabolicus A1SM3 strain was isolated from a sediment sample from Manaure Solar Saltern in La Guajira and the produced crude extracts have shown antibacterial activity against methicillin-resistant Staphylococcus aureus and cytotoxic activity against human lung cell line. Thus, the aim of this research was to identify the main compound responsible for the biological activity observed and to systematically study how each carbon and nitrogen source in the growth media, and variation of the salinity, affect its production. For the characterization of the bioactive metabolites, 15 fractions obtained from Vibrio diabolicus A1SM3 crude extract were analyzed by HPLC-MS/MS and their activity was established. The bioactive fractions were dereplicated with Antibase and Marinlit databases, which combined with nuclear magnetic resonance (NMR) spectra and fragmentation by MS/MS, led to the identification of 2,2-di(3-indolyl)-3-indolone (isotrisindoline), an indole-derivative antibiotic, previously isolated from marine organisms. The influence of the variations of the culture media in isotrisindoline production was established by molecular network and MZmine showing that the media containing starch and peptone at 7% NaCl was the best culture media to produce it. Also, polyhydroxybutyrates (PHB) identification was established by MS/MS mainly in casamino acids media, contributing to the first report on PHB production by this strain. View Full-Text
Keywords: Vibrio diabolicus; molecular networking; 2,2-di(3-indolyl)-3-indolone; PHB; MS/MS; solar saltern Vibrio diabolicus; molecular networking; 2,2-di(3-indolyl)-3-indolone; PHB; MS/MS; solar saltern
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Conde-Martínez, N.; Bauermeister, A.; Pilon, A.C.; Lopes, N.P.; Tello, E. Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by Vibrio diabolicus A1SM3. Mar. Drugs 2019, 17, 196.

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