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Wound Healing Potential of Spirulina Protein on CCD-986sk Cells

Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Korea
Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Korea
Department of Marine Bio-Materials and Aquaculture, Pukyong National University, Busan 48513, Korea
Author to whom correspondence should be addressed.
Mar. Drugs 2019, 17(2), 130;
Received: 17 January 2019 / Revised: 13 February 2019 / Accepted: 20 February 2019 / Published: 22 February 2019
(This article belongs to the Special Issue The Pharmacological Potential of Marine-Derived Peptides and Proteins)
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Wound healing is a dynamic and complex process. The proliferation and migration of dermal fibroblasts are crucial for wound healing. Recent studies have indicated that the extracts from Spirulina platensis have a positive potential for wound healing. However, its underlying mechanism is not fully understood. Our previous study showed that spirulina crude protein (SPCP) promoted the viability of human dermal fibroblast cell line (CCD-986sk cells). In this study, we further investigated the wound healing effect and corresponding mechanisms of SPCP on CCD-986sk cells. Bromodeoxyuridine (BrdU) assay showed that SPCP promoted the proliferation of CCD-986sk cells. The wound healing assay showed that SPCP promoted the migration of CCD-986sk cells. Furthermore, cell cycle analysis demonstrated that SPCP promoted CCD-986sk cells to enter S and G2/M phases from G0/G1 phase. Western blot results showed that SPCP significantly upregulated the expression of cyclin D1, cyclin E, cyclin-dependent kinase 2 (Cdk2), cyclin-dependent kinase 4 (Cdk4), and cyclin-dependent kinase 6 (Cdk6), as well as inhibited the expression of CDK inhibitors p21 and p27 in CCD-986sk cells. In the meanwhile, SPCP promoted the phosphorylation and activation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). However, the phosphorylation of Akt was significantly blocked by PI3K inhibitor (LY294002), which in turn reduced the SPCP-induced proliferation and migration of CCD-986sk cells. Therefore, the results presenting in this study suggested that SPCP can promote the proliferation and migration of CCD-986sk cells; the PI3K/Akt signaling pathway play a positive and important role in these processes. View Full-Text
Keywords: spirulina crude protein; dermal fibroblasts; wound healing; cell cycle; PI3K/Akt signaling pathway spirulina crude protein; dermal fibroblasts; wound healing; cell cycle; PI3K/Akt signaling pathway

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Liu, P.; Choi, J.-W.; Lee, M.-K.; Choi, Y.-H.; Nam, T.-J. Wound Healing Potential of Spirulina Protein on CCD-986sk Cells. Mar. Drugs 2019, 17, 130.

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