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Open AccessArticle

A Cold-Adapted Chitinase-Producing Bacterium from Antarctica and Its Potential in Biocontrol of Plant Pathogenic Fungi

by Kezhen Liu 1,2, Haitao Ding 2,*, Yong Yu 2 and Bo Chen 2,*
1
College of Marine Science, Shanghai Ocean University, Shanghai 201306, China
2
MNR Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China
*
Authors to whom correspondence should be addressed.
Mar. Drugs 2019, 17(12), 695; https://doi.org/10.3390/md17120695
Received: 14 November 2019 / Revised: 27 November 2019 / Accepted: 5 December 2019 / Published: 10 December 2019
(This article belongs to the Special Issue Bioactive Molecules from Extreme Environments)
To obtain chitinase-producing microorganisms with high chitinolytic activity at low temperature, samples collected from Fildes Peninsula in Antarctica were used as sources for bioprospecting of chitinolytic microorganisms. A cold-adapted strain, designated as GWSMS-1, was isolated from marine sediment and further characterized as Pseudomonas. To improve the chitinase production, one-factor-at-a-time and orthogonal test approaches were adopted to optimize the medium components and culture conditions. The results showed that the highest chitinolytic activity (6.36 times higher than that before optimization) was obtained with 95.41 U L−1 with 15 g L−1 of glucose, 1 g L−1 of peptone, 15 g L−1 of colloid chitin and 0.25 g L−1 of magnesium ions contained in the medium, cultivated under pH 7.0 and a temperature of 20 °C. To better understand the application potential of this strain, the enzymatic properties and the antifungal activity of the crude chitinase secreted by the strain were further investigated. The crude enzyme showed the maximum catalytic activity at 35 °C and pH 4.5, and it also exhibited excellent low-temperature activity, which still displayed more than 50% of its maximal activity at 0 °C. Furthermore, the crude chitinase showed significant inhibition of fungi Verticillium dahlia CICC 2534 and Fusarium oxysporum f. sp. cucumerinum CICC 2532, which can cause cotton wilt and cucumber blight, respectively, suggesting that strain GWSMS-1 could be a competitive candidate for biological control in agriculture, especially at low temperature. View Full-Text
Keywords: Antarctica; chitinase; cold-adapted; optimization; antifungal; Pseudomonas Antarctica; chitinase; cold-adapted; optimization; antifungal; Pseudomonas
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Liu, K.; Ding, H.; Yu, Y.; Chen, B. A Cold-Adapted Chitinase-Producing Bacterium from Antarctica and Its Potential in Biocontrol of Plant Pathogenic Fungi. Mar. Drugs 2019, 17, 695.

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